Peptic and tryptic digestion of peptides and proteins monitored by capillary electrophoresis with contactless conductivity detection

Analytical Biochemistry

Volumn 387, Issue 2, 2009, Pages 202-207

  Schuchert Shi, Aiping ^a   Hauser, Peter C ^a  

^a UNIVERSITY OF BASEL   (Switzerland)

Author keywords

Capillary electrophoresis; Contactless conductivity detection; EMMA; Off line; On line; Peptic digestion; Tryptic digestion

Indexed keywords

CAPILLARY ELECTROPHORESIS; MAMMALS;

CONTACTLESS CONDUCTIVITY DETECTION; EMMA; OFF-LINE; ON-LINE; TRYPTIC DIGESTION;

PEPTIDES;

AMINO ACID; APOMYOGLOBIN; BOVINE SERUM ALBUMIN; CYTOCHROME C; GASTRIN DERIVATIVE; HUMAN SERUM ALBUMIN; MINIGASTRIN I; MYOGLOBIN; PEPSIN A; TRYPSIN; UNCLASSIFIED DRUG;

ARTICLE; CAPACITIVELY COUPLED CONTACTLESS CONDUCTIVITY DETECTION; CAPILLARY ELECTROPHORESIS; CATALYSIS; CHEMICAL REACTION; CONCENTRATION (PARAMETERS); ELECTROPHORETICALLY MEDIATED MICROANALYSIS; HUMAN; MICROANALYSIS; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CLEAVAGE; PROTEIN DIGESTION;

BOVINAE;

EID: 61849134439     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.01.025     Document Type: Article

References (43)

1. Manz A., Pamme N., and Iossifidis D. Bioanalytical Chemistry (2004), Imperial College Press, London
2. Wilfart A., Jaguelin-Peyraud Y., Simmins H., Noblet J., van Milgen J., and Montange L. Kinetics of enzymatic digestion of feeds as estimated by a stepwise in vitro method, Anim. Feed Sci. Technol. 141 (2008) 171-183
3. Thomas K., Aalbers M., Bannon G.A., Bartels M., Dearman R.J., Esdaile D.J., Fu T.J., Glatt C.M., Hadfield N., Hatzos C., Hefle S.L., Heylings J.R., Goodman R.E., Henry B., Herouet C., Holsapple M., Ladics G.S., Landry T.D., MacIntosh S.C., Rice E.A., Privalle L.S., Steiner H.Y., Teshima R., van Ree R., Woolhiser M., and Zawodny J. A multi-laboratory evaluation of a common in vitro pepsin digestion protocol used in assessing the safety of novel proteins. Regul. Toxicol. Pharmacol. 39 (2004) 87-98
4. Schmelzer C.E.H., Schöps R., Reynell L., Ulbrich-Hofmann R., Neubert R.H.H., and Raith K. Peptic digestion of β-casein: time course and fate of possible bioactive peptides. J. Chromatogr. A 1166 (2007) 108-115
5. Massolini G., and Calleri E. Immobilized trypsin systems coupled on-line to separation methods: recent developments and analytical applications. J. Sep. Sci. 28 (2005) 7-21
6. de Bont D.B.A., Sliedregt-Bol K.M., Hofmeyer L.J.F., and Liskamp R.M.J. Increased stability of peptidesulfonamide peptidomimetics towards protease catalyzed degradation. Bioorg. Med. Chem. 7 (1999) 1043-1047
7. Lim L.W., Tomatsu M., and Takeuchi T. Development of an on-line immobilized-enzyme reversed-phase HPLC method for protein digestion and peptide separation. Anal. Bioanal. Chem. 386 (2006) 614-620
8. Bonneil E., and Waldron K.C. On-line system for peptide mapping by capillary electrophoresis at sub-micromolar concentrations. Talanta 53 (2000) 687-699
9. Mikšík I., Charvátová J., Eckhardt A., and Deyl Z. Peptide mapping by capillary electrophoresis with Pluronic F127. J. Chromatogr. B (2004) 155-160
10. Yue G.E., Roper M.G., Balchunas C., Pulsipher A., Coon J.J., Shabanowitz J., Hunt D.F., Landers J.P., and Ferrance J.P. Protein digestion and phosphopeptide enrichment on a glass microchip. Anal. Chim. Acta 564 (2006) 116-122
11. Glatz Z. Determination of enzymatic activity by capillary electrophoresis. J. Chromatogr. B 841 (2006) 23-37
12. Liu J., Pan T., Woolley A.T., and Lee M.L. Surface-modified poly(methyl methacrylate) capillary electrophoresis microchips for protein and peptide analysis. Anal. Chem. 76 (2004) 6948-6955
13. Gaš B., Zuska J., Coufal P., and van de Goor T. Optimization of the high-frequency contactless conductivity detector for capillary electrophoresis. Electrophoresis 23 (2002) 3520-3527
14. Zemann A.J. Capacitively coupled contactless conductivity detection in capillary electrophoresis. Electrophoresis 24 (2003) 2125-2137
15. Kubáň P., and Hauser P.C. Fundamental aspects of contactless conductivity detection for capillary electrophoresis: I. Frequency behavior and cell geometry. Electrophoresis 25 (2004) 3387-3397
16. Kubáň P., and Hauser P.C. Fundamental aspects of contactless conductivity detection for capillary electrophoresis: II. Signal-to-noise ratio and stray capacitance. Electrophoresis 25 (2004) 3398-3405
17. Brito-Neto J.G.A., da Silva J.A.F., Blanes L., and do Lago C.L. Understanding capacitively coupled contactless conductivity detection in capillary and microchip electrophoresis: II. Peak shape, stray capacitance, noise, and actual electronics. Electroanalysis 17 (2005) 1207-1214
18. Brito-Neto J.G.A., da Silva J.A.F., Blanes L., and do Lago C.L. Understanding capacitively coupled contactless conductivity detection in capillary and microchip electrophoresis: I. Fundamentals. Electroanalysis 17 (2005) 1198-1206
19. Johnston S.E., Fadgen K.E., Tolley L.T., and Jorgenson J.W. Expanded electrical model of a contactless conductivity detector: development and verification. J. Chromatogr. A 1094 (2005) 148-157
20. Šolinová V., and Kašička V. Recent applications of conductivity detection in capillary and chip electrophoresis. J. Sep. Sci. 29 (2006) 1743-1762
21. Kubáň P., and Hauser P.C. A review of the recent achievements in capacitively coupled contactless conductivity detection. Anal. Chim. Acta 607 (2008) 15-29
22. Coufal P., Zuska J., Van den Goor T., and Gaš B. Separation of twenty underivatized essential amino acids by capillary zone electrophoresis with contactless conductivity detection. Electrophoresis 24 (2003) 671-677
23. Tanyanyiwa J., Schweizer K., and Hauser P.C. High-voltage contactless conductivity detection of underivatized amino acids in capillary electrophoresis. Electrophoresis 24 (2003) 2119-2124
24. Harrison S.M., Kaml I., Prokoratova V., Mazanek M., and Kenndler E. Animal glues in mixtures of natural binding media used in artistic and historic objects: identification by capillary zone electrophoresis. Anal. Bioanal. Chem. 382 (2005) 1520-1526
25. Samcová E., and Tůma P. Determination of proteinogenic amino acids in human plasma by capillary electrophoresis with contactless conductivity detection. Electroanalysis 18 (2006) 152-157
26. Tůma P., Samcová E., and Andělová K. Determination of free amino acids and related compounds in amniotic fluid by capillary electrophoresis with contactless conductivity detection. J. Chromatogr. B 839 (2006) 12-18
27. Gong X.Y., Dobrunz D., Kümin M., Wiesner M., Revell J.D., Wennemers H., and Hauser P.C. Separating stereoisomers of di-, tri-, and tetrapeptides using capillary electrophoresis with contactless conductivity detection. J. Sep. Sci. 31 (2008) 565-573
28. Abad-Villar E.M., Tanyanyiwa J., Fernández-Abedul M.T., Costa-García A., and Hauser P.C. Detection of human immunoglobulin in microchip and conventional capillary electrophoresis with high-voltage contactless conductivity detection. Anal. Chem. 76 (2004) 1282-1288
29. Abad-Villar E.M., Kubáň P., and Hauser P.C. Evaluation of the detection of biomolecules in capillary electrophoresis by contactless conductivity measurement. J. Sep. Sci. 29 (2006) 1031-1037
30. Xu Y., and Li S.F.Y. Carbon nanotube-enhanced separation of DNA fragments by a portable capillary electrophoresis system with contactless conductivity detection. Electrophoresis 27 (2006) 4025-4028
31. Schuchert-Shi A., Kubáň P., and Hauser P.C. Monitoring of enzymatic reactions using conventional and on-chip capillary electrophoresis with contactless conductivity detection. Electrophoresis 28 (2007) 4690-4696
32. Urban P.L., Goodall D.M., Carvalho A.Z., Bergström E.T., Van Schepdael A., and Bruce N.C. Multi-compound electrophoretic assays for tyramine oxidase with a UV area detector imaging multiple windows on a loop. J. Chromatogr. A 1206 (2008) 52-63
33. Schuchert-Shi A., and Hauser P.C. Monitoring the enzymatic conversion of urea to ammonium by conventional or microchip capillary electrophoresis with contactless conductivity detection. Anal. Biochem. 376 (2008) 262-267
34. Tanyanyiwa J., and Hauser P.C. High voltage contactless conductivity detection of metal ions in capillary electrophoresis. Electrophoresis 23 (2002) 3781-3786
35. Nakashima T., Higa H., Matsubara H., Benson A.M., and Yasunobu K.T. The amino acid sequence of bovine heart cytochrome c. J. Biol. Chem. 241 (1966) 1166-1177
36. Craft D., Doucette A., and Li L. Microcolumn capture and digestion of proteins combined with mass spectrometry for protein identification. J. Proteome Res. 1 (2002) 537-547
37. Zhao C., Jiang H., Smith D.R., Bruckenstein S., and Wood T.D. Integration of an on-line protein digestion microreactor to a nanoelectrospray emitter for peptide mapping. Anal. Biochem. 359 (2006) 167-175
38. Al-Lawati H., Watts P., and Welham K.J. Efficient protein digestion with peptide separation in a micro-device interfaced to electrospray mass spectrometry. Analyst 131 (2006) 656-663
39. Bao J., and Regnier F.E. Ultramicro enzyme assays in a capillary electrophoretic system. J. Chromatogr. 608 (1992) 217-224
40. Regnier F.E., Patterson D.H., and Harmon B.J. Electrophoretically-mediated microanalysis (EMMA). Trends Anal. Chem. 14 (1995) 177-181
41. Zhang J., Hoogmartens J., and Van Schepdael A. Advances in capillary electrophoretically mediated microanalysis: an update. Electrophoresis 27 (2006) 35-43
42. Van Dyck S., Van Schepdael A., and Hoogmartens J. Michaelis-Menten analysis of bovine plasma amine oxidase by capillary electrophoresis using electrophoretically mediated microanalysis in a partially filled capillary. Electrophoresis 22 (2001) 1436-1442
43. Pohlentz G., Kölbl S., and Peter-Katalinić J. High sequence coverage by in-capillary proteolysis of native proteins and simultaneous analysis of the resulting peptides by nanoelectrospray ionization-mass spectrometry and tandem mass spectrometry. Proteomics 5 (2005) 1758-1763