Comparative evaluation of eight serological assays for diagnosing Chlamydophila abortus infection in sheep

Veterinary Microbiology

Volumn 135, Issue 1-2, 2009, Pages 38-45

  Wilson, Kim ^a   Livingstone, Morag ^a   Longbottom, David ^a  

^a MOREDUN RESEARCH INSTITUTE   (United Kingdom)

Author keywords

Chlamydophila abortus; Chlamydophila pecorum; Indirect ELISA; Ovine enzootic abortion; Polymorphic outer membrane proteins; Serological diagnosis

Indexed keywords

OUTER MEMBRANE PROTEIN;

ABORTION; ARTICLE; CHLAMYDOPHILA; CHLAMYDOPHILA ABORTUS; CHLAMYDOPHILA INFECTION; CHLAMYDOPHILA PECORUM; COMPLEMENT FIXATION TEST; CONTROLLED STUDY; CROSS REACTION; ENZYME LINKED IMMUNOSORBENT ASSAY; NONHUMAN; SENSITIVITY AND SPECIFICITY; SERODIAGNOSIS; SHEEP;

ABORTION, VETERINARY; ANIMALS; BACTERIAL PROTEINS; CHLAMYDOPHILA; CHLAMYDOPHILA INFECTIONS; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; PREGNANCY; SENSITIVITY AND SPECIFICITY; SEROLOGIC TESTS; SHEEP; SHEEP DISEASES;

ANIMALIA; CHLAMYDOPHILA; CHLAMYDOPHILA ABORTUS; CHLAMYDOPHILA PECORUM; OVIS; OVIS ARIES;

EID: 61749094343     PISSN: 03781135     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetmic.2008.09.043     Document Type: Article

References (20)

1. Aitken I.D., and Longbottom D. Enzootic abortion of ewes (ovine chlamydiosis). In: OIE Biological Standards Commission (Ed). Manual Of Diagnostic Tests And Vaccines For Terrestrial Animals (Mammals, Birds And Bees) (2004), Office International des Epizooties, Paris 635-641
2. Anderson I.E., Baxter S.I.F., Dunbar S., Rae A.G., Philips H.L., Clarkson M.J., and Herring A.J. Analyses of the genomes of chlamydial isolates from ruminants and pigs support the adoption of the new species Chlamydia pecorum. Int. J. Syst. Bacteriol. 46 (1996) 245-251
3. Anderson I.E., Herring A.J., Jones G.E., Low J.C., and Greig A. Development and evaluation of an indirect ELISA to detect antibodies to abortion strains of Chlamydia psittaci in sheep sera. Vet. Microbiol. 43 (1995) 1-12
4. Buendia A.J., Cuello F., Del Rio L., Gallego M.C., Caro M.R., and Salinas J. Field evaluation of a new commercially available ELISA based on a recombinant antigen for diagnosing Chlamydophila abortus (Chlamydia psittaci serotype 1) infection. Vet. Microbiol. 78 (2001) 229-239
5. Fukushi H., and Hirai K. Chlamydia pecorum-the fourth species of genus Chlamydia. Microbiol. Immunol. 37 (1993) 516-522
6. Griffiths P.C., Plater J.M., Horigan M.W., Rose M.P.M., Venables C., and Dawson M. Serological diagnosis of ovine enzootic abortion by comparative inclusion immunofluorescence assay, recombinant lipopolysaccharide enzyme-linked-immunosorbent-assay, and complement-fixation test. J. Clin. Microbiol. 34 (1996) 1512-1518
7. Hoelzle L.E., Hoelzle K., and Wittenbrink M.M. Recombinant major outer membrane protein (MOMP) of Chlamydophila abortus, Chlamydophila pecorum, and Chlamydia suis as antigens to distinguish chlamydial species-specific antibodies in animal sera. Vet. Microbiol. 103 (2004) 85-90
8. Jones G.E., Low J.C., Machell J., and Armstrong K. Comparison of five tests for the detection of antibodies against chlamydial (enzootic) abortion of ewes. Vet. Rec. 141 (1997) 164-168
9. Kaltenboeck B., Heard D., Degraves F.J., and Schmeer N. Use of synthetic antigens improves detection by enzyme-linked immunosorbent assay of antibodies against abortigenic Chlamydia psittaci in ruminants. J. Clin. Microbiol. 35 (1997) 2293-2298
10. Livingstone M., Entrican G., Wattegedera S., Buxton D., McKendrick I.J., and Longbottom D. Antibody responses to recombinant protein fragments of the major outer membrane protein and polymorphic outer membrane protein POMP90 in Chlamydophila abortus-infected pregnant sheep. Clin. Diagn. Lab. Immunol. 12 (2005) 770-777
11. Longbottom D., and Coulter L.J. Animal chlamydioses and zoonotic implications. J. Comp. Pathol. 128 (2003) 217-244
12. Longbottom D., Fairley S., Chapman S., Psarrou E., Vretou E., and Livingstone M. Serological diagnosis of ovine enzootic abortion by enzyme-linked immunosorbent assay using a recombinant protein fragment of the polymorphic outer membrane protein POMP90 of Chlamydophila abortus. J. Clin. Microbiol. 40 (2002) 4235-4243
13. Longbottom D., Psarrou E., Livingstone M., and Vretou E. Diagnosis of ovine enzootic abortion using an indirect ELISA (rOMP91B iELISA) based on a recombinant protein fragment of the polymorphic outer membrane protein POMP91B of Chlamydophila abortus. FEMS. Microbiol. Lett. 195 (2001) 157-161
14. Markey B.K., McNulty M.S., and Todd D. Comparison of serological tests for the diagnosis of Chlamydia psittaci infection of sheep. Vet. Microbiol. 36 (1993) 233-252
15. Markey B.K., McNulty M.S., Todd D., and Mackie D.P. Comparison of ovine abortion and non-abortion isolates of Chlamydia psittaci using inclusion morphology, polyacrylamide gel electrophoresis, restriction endonuclease analysis and reactivity with monoclonal antibodies. Vet. Microbiol. 35 (1993) 141-159
16. McCauley L.M.E., Lancaster M.J., Young P., Butler K.L., and Ainsworth C.G.V. Comparison of ELISA and CFT assays for Chlamydophila abortus antibodies in ovine sera. Aust. Vet. J. 85 (2007) 325-328
17. McClenaghan M., Herring A.J., and Aitken I.D. Comparison of Chlamydia psittaci isolates by DNA restriction endonuclease analysis. Infect. Immun. 45 (1984) 384-389
18. Salti-Montesanto V., Tsoli E., Papavassiliou P., Psarrou E., Markey B.K., Jones G.E., and Vretou E. Diagnosis of ovine enzootic abortion, using a competitive ELISA based on monoclonal antibodies against variable segments 1 and 2 of the major outer membrane protein of Chlamydia psittaci serotype 1. Am. J. Vet. Res. 58 (1997) 228-235
19. Stamp J.T., Watt J.A.A., and Cockburn R.B. Enzootic abortion in ewes: complement fixation test. J. Comp. Pathol. 62 (1952) 93-101
20. Vretou E., Radouani F., Psarrou E., Kritikos I., Xylouri E., and Mangana O. Evaluation of two commercial assays for the detection of Chlamydophila abortus antibodies. Vet. Microbiol. 123 (2007) 153-161