Engineering, cloning, and functional characterization of recombinant LIM mineralization protein-1 containing an N-terminal HIV-derived membrane transduction domain

Protein Expression and Purification

Volumn 65, Issue 2, 2009, Pages 165-173

  Sangadala, Sreedhara ^a   Okada, Motohiro ^a   Liu, Yunshan ^a   Viggeswarapu, Manjula ^a   Titus, Louisa ^a   Boden, Scott D ^a  

^a EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

LMP 1; Membrane transduction domain; Osteogenic; TAT

Indexed keywords

BONE MORPHOGENETIC PROTEIN; FLUORESCENT DYE; HYBRID PROTEIN; PDLIM7 PROTEIN, HUMAN; SIGNAL PEPTIDE; TRANSACTIVATOR PROTEIN;

AFFINITY CHROMATOGRAPHY; ANIMAL; ARTICLE; BINDING SITE; CELL LINE; CHEMISTRY; ESCHERICHIA COLI; FLOW CYTOMETRY; GENETIC TRANSDUCTION; GENETICS; METABOLISM; MOLECULAR CLONING; MOUSE; PROTEIN ENGINEERING; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SPECTROFLUOROMETRY; WESTERN BLOTTING;

ANIMALS; BINDING SITES; BLOTTING, WESTERN; BONE MORPHOGENETIC PROTEINS; CELL LINE; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; ESCHERICHIA COLI; FLOW CYTOMETRY; FLUORESCENT DYES; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; MICE; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SPECTROMETRY, FLUORESCENCE; TAT GENE PRODUCTS, HUMAN IMMUNODEFICIENCY VIRUS; TRANSDUCTION, GENETIC;

HUMAN IMMUNODEFICIENCY VIRUS; MAMMALIA; ORYCTOLAGUS CUNICULUS;

EID: 61749094225     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2009.01.006     Document Type: Article

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