Simple broad-spectrum protocol using labiase for bacterial cell lysis to prepare genomic DNA for pulsed-field gel electrophoresis analysis

Microbiology and Immunology

Volumn 53, Issue 1, 2009, Pages 45-48

  Oana, Kozue ^a   Kawakami, Yoshiyuki ^a   Hayashi, Tetsuya ^b,c   Ohnishi, Makoto ^d  

^a SHINSHU UNIVERSITY   (Japan)

^b UNIVERSITY OF MIYAZAKI   (Japan)

^c UNIVERSITY OF MIYAZAKI   (Japan)

^d NATIONAL INSTITUTE OF INFECTIOUS DISEASES   (Japan)

Author keywords

Labiase; Lytic enzyme; Pulsed field gel electrophoresis

Indexed keywords

GENOMIC DNA; HYDROLASE; LABIASE; UNCLASSIFIED DRUG; BACTERIAL DNA; BACTERIAL PROTEIN; LYSOZYME;

ARTICLE; BACTERIAL CELL; BACTERIAL CELL LYSIS; BACTERIAL STRAIN; CYTOLYSIS; ENTEROCOCCUS; ENZYME ACTIVITY; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; NONHUMAN; PULSED FIELD GEL ELECTROPHORESIS; BACTERIAL GENOME; BACTERIUM; ENZYMOLOGY; EVALUATION; GENETIC PROCEDURES; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; STREPTOMYCES;

BACTERIA (MICROORGANISMS); NEGIBACTERIA;

BACTERIA; BACTERIAL PROTEINS; DNA, BACTERIAL; ELECTROPHORESIS, GEL, PULSED-FIELD; GENETIC TECHNIQUES; GENOME, BACTERIAL; MURAMIDASE; STREPTOMYCES;

EID: 61749083331     PISSN: 03855600     EISSN: 13480421     Source Type: Journal    
DOI: 10.1111/j.1348-0421.2008.00084.x     Document Type: Article

References (7)

1. Fey P.D., Rupp M.E. (2003) Molecular epidemiology in the public health and hospital environments. Clin Lab Med 23: 885-901.
2. Schleifer K.H., Kandler O. (1972) Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36: 407-77.
3. Ezaki T., Saidi S.M., Liu S.L., Hashimoto Y., Yamamoto H., Yabuuchi E. (1990) Rapid procedure to determine the DNA base composition from small amounts of Gram-positive bacteria. FEMS Microbiol Lett 67: 127-30.
4. Ohbuchi K., Hasegawa K., Hamachi M., Ozeki K., Kumagai C. (2001) Isolation of a new lytic enzyme for hiochi bacteria and other lactic acid bacteria. J Biosci Bioeng 91: 487-92.
5. Niwa T., Kawamura Y., Katagiri Y., Ezaki T. (2005) Lytic enzyme, labiase for a broad range of Gram-positive bacteria and its application to analyze functional DNA/RNA. J Microbiol Methods 61: 251-60.
6. Furihata K., Sato K., Matsumoto H. (1995) Construction of a combined Not I/SmaI physical and genetic map of Moraxella (Branhamella) catarrhalis strain ATCC25238. Microbiol. Immunol. 39: 745-51.
7. Oana K., Kawakami Y., Ohnishi M., Ishikawa M., Hirota M., Tozuka M. et al. (2001) Molecular and epidemiological study of the first outbreak of vanB type vancomycin-resistant Enterococcus faecalis in Japan. Jpn J Infect Dis 54: 17-22.