Chef procedures: A rapid high-temperature method for sample preparation, a high voltage hepes buffer system and the use of nusieve® agarose

Journal of Rapid Methods and Automation in Microbiology

Volumn 17, Issue 1, 2009, Pages 9-16

  Kinscherf, Thomas G ^a,b   Yap, Mee Ngan ^b   Charkowski, Amy O ^b   Willis, David K ^a,b  

^a U S DAIRY FORAGE RESEARCH CENTER   (United States)

^b UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA (MICROORGANISMS); NEGIBACTERIA;

EID: 61649105506     PISSN: 10603999     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1745-4581.2008.00147.x     Document Type: Article

References (10)

1. BESTA, R.M. FRANK, M.B. 1997. Diethylpyrocarbonate (DEPC) treatment of aqueous solutions. In Molecular Biology Protocols (M.B. Frank, ed.) http://www.omrf.ouhsc.edu/OMRF/Research/09/FrankLab/TOC.HTML (accessed October 27, 2008).
2. BIO-RAD. 1992. CHEF-DRII Pulsed Field Electrophoresis Systems-Instruction Manual and Applications Guide. Bio-Rad Laboratories, Hercules, CA.
3. COOPER, K.L., LUEY, C.K., BIRD, M., TERAJIMA, J., NAIR, G.B., KAM, K.M., ARAKAWA, E., SAFA, A., CHEUNG, D.T., LAW, C.P., ET AL. 2006. Development and validation of a PulseNet standardized pulsed-field gel electrophoresis protocol for subtyping of Vibrio cholerae. Foodborne Pathog. Dis. 3, 51 58.
4. KOORT, J.M., LUKINMAA, S., RANTALA, M., UNKILA, E. SIITONEN, A. 2002. Technical improvement to prevent DNA degradation of enteric pathogens in pulsed-field gel electrophoresis. J. Clin. Microbiol. 40, 3497 3498.
5. OLSON, M.V. 1989. Separation of large DNA molecules by pulsed-field gel electrophoresis. A review of the basic phenomenology. J. Chromatogr. 470, 377 383.
6. RAY, T., WEADEN, J. DYSON, P. 1992. Tris-dependent site-specific cleavage of Streptomyces lividans DNA. FEMS Microbiol. Lett. 75, 247 252.
7. RAY, T., MILLS, A. DYSON, P. 1995. Tris-dependent oxidative DNA strand scission during electrophoresis. Electrophoresis 16, 888 894.
8. RIBOT, E.M., FAIR, M.A., GAUTOM, R., CAMERON, D.N., HUNTER, S.B., SWAMINATHAN, B. BARRETT, T.J. 2006. Standardization of pulsed-field gel electrophoresis protocols for the subtyping of Escherichia coli O157:H7, Salmonella, and Shigella for PulseNet. Foodborne Pathog. Dis. 3, 59 67.
9. YAP, M.N., BARAK, J.D. CHARKOWSKI, A.O. 2004. Genomic diversity of Erwinia carotovora subsp. carotovora and its correlation with virulence. Appl. Environ. Microbiol. 70, 3013 3023.
10. ZHANG, Y., YAKRUS, M.A., GRAVISS, E.A., WILLIAMS-BOUYER, N., TURENNE, C., KABANI, A. WALLACE, R.J., JR. 2004. Pulsed-field gel electrophoresis study of Mycobacterium abscessus isolates previously affected by DNA degradation. J. Clin. Microbiol. 42, 5582 5587.