Quenching of fluorescence by crystal violet and its use to differentiate between surface-bound and internalized bacteria

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

Volumn 6791, Issue , 2008, Pages

  Mathew, S ^a   Lim, Y C ^b   Kishen, A ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^b NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Acridine orange crystal violet staining; Biofilm; Intracellular survival

Indexed keywords

ANTIGEN-ANTIBODY REACTIONS; BIOFILMS; BIOFILTERS; CELL CULTURE; CELL MEMBRANES; FLUORESCENCE; MACROPHAGES; NUTRIENTS; OPTICAL INSTRUMENTS; QUENCHING;

ACRIDINE ORANGE-CRYSTAL VIOLET STAINING; ATTACHED BACTERIA; BACTERIAL BIOFILMS; BACTERIAL CELLS; CELL LINES; CELL SURFACES; COMPLEX PROCESS; CULTURE MEDIUMS; CULTURED CELLS; ENTEROCOCCUS FAECALIS; FLUORESCENT LIGHTS; GLASS SUBSTRATES; INTRACELLULAR BACTERIA; INTRACELLULAR SURVIVAL; MODEL SYSTEMS; NUTRIENT-DEPRIVED; PHOSPHATE-BUFFERED SALINES; RICH CONDITIONS;

BACTERIOLOGY;

EID: 61649093025     PISSN: 16057422     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.803971     Document Type: Conference Paper

References (10)

1. Elsbach P. Interaction between phagocytes and microorganisms. New Eng. J. Med 1973: 289: 846-855.
2. Simberkoff MS, Elsbach P, Interaction in-vitro between polymorphonuclear leukocytes and mycoplasma. J. Exp. Med 1971:134:1417-1422.
3. Horwitz MA. Phagocytosis of microorganisms. Rev. Infect.Dis 1982:4:104-123.
4. Sveum RJ, Chused TM, Frank MM Brown EJ. A quantitative fluorescent method for measurement of bacterial adherence and phagocytosis. J. Immunol. Methods. 1986:90:257-264.
5. Bjerknes R, Baswe C-F. Human leukocyte phagocytosis of zymosan particles measured by flow cytometry. Acta Path Microbial Immunol Scand.1983:91:341-348.
6. Hed J. The extinction of fluorescence by crystal violet and its use to differentiate between attached and ingested microorganisms in phagocytosis. FEMS Microbiology Letters.1977:1:357-361.
7. Hed J. Methods for distinguishing ingested from adhering particles. Methods Enzymol. 1986: 132: 198-203. 8 Heesemann J,Laufs R. Double Immunofluorescence Microscopic Technique for Accurate Differentiation of Extracellularly and Intracellularly Located Bacteria in Cell Culture. J Clin Microbiol. 1985:168-175.
8. Sigurd D, Süßmuth, Muscholl-Silberhorn A, Wirth R, Susa, Marre Reinhard, and Rozdzinski E.Aggregation Substance Promotes Adherence, Phagocytosis, and Intracellular Survival of Enterococcus faecalis within Human Macrophages and Suppresses Respiratory Burst.Infect Immun. 2000:68(9): 4900-4906.
9. Giannelia R A, Washington P, Gemski, Formal SB. Invasion of HeLa cells by Salmonella typhimurium: a model for study of invasiveness of Salmonella. J. Infect. Dis. 1973:128:69-75.
10. Lamanna C, Malette MF, Zimmerman L. Basic Bacteriology.4th ed. Baltimore, Md. 1971:148-160.