Optimization of RNA isolation from brittle leaf disease affected date palm leaves and construction of a subtractive cDNA library

Molecular Biotechnology

Volumn 41, Issue 1, 2009, Pages 63-68

  Saïdi, Mohammed Najib ^a   Gargouri Bouzid, Radhia ^a   Rayanni, Mariem ^a   Drira, Noureddine ^b  

^a UNIVERSITY OF SFAX   (Tunisia)

^b Faculté des Sciences de Sfax   (Tunisia)

Author keywords

Date palm leaves; Phenolic compounds; RNA isolation; Subtractive hybridization

Indexed keywords

PHENOLS; RNA;

CDNA SYNTHESIS; CYTOPLASMIC RNAS; DATE PALM LEAVES; DIFFERENTIAL SCREENINGS; EFFICIENT METHODS; HIGH QUALITIES; LEAF DISEASE; MERCAPTOETHANOL; NON-IONIC DETERGENTS; PHENOLIC COMPOUNDS; POLYVINYL-PYRROLIDONE; RNA ISOLATION; SUBTRACTIVE CDNA LIBRARIES; SUBTRACTIVE HYBRIDIZATION;

NUCLEIC ACIDS;

ARTICLE; BRITTLE LEAF DISEASE; CONTROLLED STUDY; DATE PALM; DNA LIBRARY; NONHUMAN; PLANT DISEASE; PLANT LEAF; POLYMERASE CHAIN REACTION; RNA EXTRACTION; RNA ISOLATION; TREE; ULTRAVIOLET SPECTROPHOTOMETRY; ARECACEAE; CHEMISTRY; GENE EXPRESSION REGULATION; GENE LIBRARY; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; NUCLEIC ACID HYBRIDIZATION;

PHOENIX DACTYLIFERA;

FLAVONOID; MACROGOL DERIVATIVE; MERCAPTOETHANOL; NONIDET P-40; PHENOL DERIVATIVE; PLANT RNA; POLYPHENOLS; POVIDONE; TRITON X 114; UNCLASSIFIED DRUG;

ARECACEAE; FLAVONOIDS; GENE EXPRESSION REGULATION, PLANT; GENE LIBRARY; MERCAPTOETHANOL; NUCLEIC ACID HYBRIDIZATION; PHENOLS; PLANT DISEASES; PLANT LEAVES; POLYETHYLENE GLYCOLS; POVIDONE; RNA, PLANT;

EID: 61449365608     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12033-008-9104-1     Document Type: Article

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