Preanalytic removal of human DNA eliminates false signals in general 16S rDNA PCR monitoring of bacterial pathogens in blood

Comparative Immunology, Microbiology and Infectious Diseases

Volumn 32, Issue 3, 2009, Pages 207-219

  Handschur, Michael ^a   Karlic, Heidrun ^a   Hertel, Christian ^b   Pfeilstöcker, Michael ^c   Haslberger, Alexander G ^d  

^a LUDWIG BOLTZMANN INSTITUTE FOR LUNG VASCULAR RESEARCH   (Austria)

^b UNIVERSITY OF HOHENHEIM   (Germany)

^c HANUSCH HOSPITAL   (Austria)

^d UNIVERSITY OF VIENNA   (Austria)

Author keywords

16S rDNA; Bacterial blood stream infection; Human DNA background; PCR; Unspecific signals

Indexed keywords

BACTERIAL DNA; DNA 16S;

ARTICLE; BACTERIUM DETECTION; BLOOD ANALYSIS; BLOOD CULTURE; CLINICAL ARTICLE; CONTROLLED STUDY; DNA EXTRACTION; FALSE POSITIVE RESULT; HUMAN; INTERMETHOD COMPARISON; NONHUMAN; POLYMERASE CHAIN REACTION; REAL TIME POLYMERASE CHAIN REACTION; RELIABILITY; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; SYSTEMIC INFLAMMATORY RESPONSE SYNDROME;

BACTERIA; DNA, BACTERIAL; DNA, RIBOSOMAL; HUMANS; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SENSITIVITY AND SPECIFICITY; SYSTEMIC INFLAMMATORY RESPONSE SYNDROME;

BACTERIA (MICROORGANISMS);

EID: 61449237550     PISSN: 01479571     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cimid.2007.10.005     Document Type: Article

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