Genetic transformation of buckwheat (Fagopyrum esculentum moench) with AtNHX1 gene and regeneration of salt-tolerant transgenic plants

Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology

Volumn 23, Issue 1, 2007, Pages 51-60

  Cheng, Li Hong ^a   Zhang, Bo ^a   Xu, Zi Qin ^a  

^a NORTHWEST UNIVERSITY   (China)

Author keywords

Fagopyrum esculentum Moench; HPLC; Na+ H+ antiporter; Rutin; Salt tolerance

Indexed keywords

AGROBACTERIUM; ARABIDOPSIS; ARABIDOPSIS THALIANA; FAGOPYRUM ESCULENTUM; RHIZOBIUM;

ARABIDOPSIS PROTEIN; CATION TRANSPORT PROTEIN; NHX1 PROTEIN, ARABIDOPSIS; POTASSIUM; PROLINE; RUTOSIDE; SODIUM; SODIUM CHLORIDE; SODIUM PROTON EXCHANGE PROTEIN;

ADAPTATION; ARTICLE; DRUG EFFECT; FAGOPYRUM; GENETIC TRANSFORMATION; GENETICS; METABOLISM; NORTHERN BLOTTING; PHYSIOLOGY; PLANT ROOT; PLANT STEM; REGENERATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING; TRANSGENIC PLANT;

ADAPTATION, PHYSIOLOGICAL; ARABIDOPSIS PROTEINS; BLOTTING, NORTHERN; BLOTTING, SOUTHERN; CATION TRANSPORT PROTEINS; FAGOPYRUM; PLANT ROOTS; PLANT STEMS; PLANTS, GENETICALLY MODIFIED; POTASSIUM; PROLINE; REGENERATION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RUTIN; SODIUM; SODIUM CHLORIDE; SODIUM-HYDROGEN ANTIPORTER; TRANSFORMATION, GENETIC;

EID: 61449203674     PISSN: 10003061     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (25)

1. Rhoades JD, Loveday J. Salinity in irrigated agriculture. In: Steward BA, Niesen DR (eds). American Society of Civil Engineers, Irrigation of Agricultural Crops. American Society of Agronomist, 1990, pp. 1089-1142.
2. Hasegawa PM, Bressan RA, Zhu JK. Plant cellular and molecular responses to high salinity. Annu Rev Plant Physiol Plant Mol Biol, 2000, 51: 486-488.
3. Zhu JK. Plant salt tolerance. Trends Plant Sci, 2001, 6: 66-71.
4. Wang BS, Zou Q. Effects of NaCl stress on the tonoplast ATPase and PPase activity in roots, sheaths and blades of sorghum seedlings. Acta Phytophysiologica Sinica, 2000, 26(3): 181-188.
5. Wang BS, Lttge U, Ratajczak R. Effects of salt treatment and osmotic stress on V-ATPase and V-PPase in leaves of the halophyte Suaeda salsa. J Exp Bot, 2001, 52: 1-11.
6. + antiportor in Arabidopsis. Science, 1999, 285: 1256-1258.
7. Gaxiola RA, Rao R, Sherman A. The Arabidopsis thaliana proton transporters, AtNHX1 and Avp1, can function in cation detoxification in yeast. Proc Natl Acad Sci USA, 1999, 96: 1480-1485.
8. + antiporters. FEBS Lett, 2000, 471: 224-228.
9. Zhang HX, Blumwald E. Transgenic salt tolerant tomato plants accumulate salt in foliage but not in fruit. Nat Biotechnol, 2001, 19: 765-768.
10. Blumwald E, Aharon GS, Apse MP. Sodium transport in plant cells. Biochim Biophys Acta, 2000, 1465: 140-151.
11. Niu X, Bressan RA, Hasegawa PM. Ion homeostasis in NaCl stress environments. Plant Physiol, 1995, 109: 735-742.
12. Sun T, Ho CT. Antioxidant activities of buckwheat extracts. Food Chemistry, 2005, 90: 743-749.
13. Wang HY, Li Y. Research status and applied prospect of buckwheat. Food Science, 2004, 25: 388-391.
14. + antiporter gene. Plant Science, 2005, 169: 65-73.
15. Wei Q. The experimental guide of molecular biology. BeiJing: BeiJing High Education Publication, Springer-Verlag, 1999.
16. Li YY, Wang ZH, Zhang Z. An optimum method of extracting buckwheat genomic RNA. Biotechnology, 2004, 14(3): 23-24.
17. Bates LS, Waldren RP, Teare ID. Rapid determination of proline for water-stress studies. Plant Soil, 1973, 39: 205-207.
18. Kim KH, Lee YH, Kim D, Park YH, Lee JY, Hwang YS, Kim YH. Agrobacterium-mediated genetic transformation of Perilla frutescens. Plant Cell Rep, 2004, 23: 386-390.
19. Wu GT, Hu ZH, Lang CX, et al. Establishment of Agrobacterium-mediated transformation system for tall fescue. Acta Agriculturae Nucleatae Sinica, 2005, 19(5): 340-346.
20. Wang Y, Zeng YL, He B, et al. Research of NHX gene transformation in Brassica napus by Agrobacterium tumefaciens. Acta Agronomica Sinica, 2006, 32(2): 278-282.
21. Hu FR. Optimization of Agrobacterium-mediated transformation of tall fescue (Festuca arundinacea Schreb). Molecular Plant Breeding, 2005, 3(3): 375-380.
22. Han XL, Bu HY, Hao JG, Zhao YW, Jia JF. Hairy root induction and plant regeneration of crownvetch (Coronilla varia L.) transformed by Agrobacterium rhizogenes. Chinese Journal of Biotechnology, 2006, 22(1): 107-113.
23. Zhang HX, Hodson JN, Williams JP, Blumwald E. Engineering salt-tolerant Brassica plant: characterization of yield and seed oil quality in transgenic plants with increased vacuolar sodium accumulation. PNAS, 2001, 98(22): 12832-12836.
24. Xu T, Zhao BC, Ge RC, et al. Introduce Tagsk1 into salt-sensitive callus to improve the capacity of salt-tolerance by micropartical bombardment. Chinese Journal of Biotechnology, 2006, 22(2): 211-214.
25. +. Plant Science, 2004, 167: 849-859.