Fluorescent assay based on resazurin for detection of activity of disinfectants against bacterial biofilm

Applied Microbiology and Biotechnology

Volumn 82, Issue 4, 2009, Pages 773-783

  Mariscal, Alberto ^a   Lopez Gigosos, Rosa M ^a   Carnero Varo, Manuel ^b   Fernandez Crehuet, Joaquin ^a,b  

^a UNIVERSIDAD DE MÁLAGA   (Spain)

^b University Clinic Hospital   (Spain)

Author keywords

Biofilm; Disinfectant; Fluorescence; Resazurin

Indexed keywords

ANALYTIC SIGNALS; ANTI-BACTERIAL ACTIVITIES; BACTERIAL ACTIVITIES; BACTERIAL BIOFILMS; BACTERIAL SPECIES; BIOFILM CELLS; BIOFILM GROWTHS; CLINICAL DEVICES; FLUORESCENT ASSAYS; INOCULA; LOG REDUCTIONS; METABOLIC ACTIVITIES; RESAZURIN; RESORUFIN; STANDARD CURVES; TEST CONDITIONS; WHOLE CELLS;

BACTERIA; BACTERIOLOGY; BIOFILMS; BIOFILTERS; ETHANOL; FLUORESCENCE; GROWTH (MATERIALS); METABOLISM;

DISINFECTANTS;

BACTERIUM; BIOASSAY; BIOFILM; CATABOLISM; DETECTION METHOD; DISINFECTION; DYE; RESPIRATION;

BACTERIA; BACTERIAL PHYSIOLOGICAL PHENOMENA; BIOFILMS; DISINFECTANTS; OXAZINES; OXIDATION-REDUCTION; SPECTROMETRY, FLUORESCENCE; XANTHENES;

BACTERIA (MICROORGANISMS);

EID: 61449201753     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-009-1879-x     Document Type: Article

References (35)

1. F Abbondanzi A Cachada T Campisi R Guerra M Raccagni A Iacondini 2003 Optimisation of a microbial bioassay for contaminated soil monitoring: bacterial inoculum standardisation and comparison with Microtox® assay Chemosphere 53 889 897
2. AFNOR. (1995) Antiseptiques et désinfectants. NF T 72-150
3. K Buckingham-Meyer D Goeres M Hamilton 2007 Comparative evaluation of biofilm disinfectant efficacy tests J Microbiol Methods 70 236 244
4. B Carpentier O Cerf 1993 Biofilms and their consequences, with particular reference to hygiene in the food industry J Appl Bacteriol 75 499 511
5. H Ceri ME Olson C Stremick RR Read D Morck A Buret 1999 The calgary biofilm device: new technology for rapid determination of antibiotic susceptibilities of bacterial biofilms J Clin Microbiol 37 1771 1776
6. GD Christensen WA Simpson JJ Younger 1985 Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices J Clin Microbiol 22 996 1006
7. JW Costerton 1999 Introduction to biofilm Int J Antimicrob Agents 11 217 221
8. D Djordjevic M Wiedmann LA McLandsborough 2002 Microtiter plate assay for assessment of Listeria monocytogenes biofilm formation Appl Environ Microbiol 68 2950 2958 doi:10.1128/AEM.68.6.2950-2958.2002
9. RM Donlan JW Costerton 2002 Biofilms: survival mechanisms of clinically relevant microorganisms Clin Microbiol Rev 15 167 193 doi:10.1128/CMR.15.2.167- 193.2002
10. European Standardization Committee (1997) Norma española UNE-EN 1040. Antisépticos y desinfectantes químicos. Actividad bactericida básica. Método de ensayo y requisitos (fase 1). AENOR, Madrid
11. TF Guerin M Mondido B McClenn B Peasley 2001 Application of resazurin for estimating abundance of contaminant-degrading microorganisms Lett Appl Microbiol 32 340 345
12. G Jackson H Beyenal WM Rees Z Lewandowski 2001 Growing reproducible biofilms with respect to structure and viable cell counts J Microbiol Methods 47 1 10
13. B Jessen L Lammert 2003 Biofilm and disinfection in meat processing plants Int Biodeterior Biodegrad 51 265 269
14. SBI Luppens MW Reij RWL Van der Heijden FM Rombouts T Abee 2002 Development of a standard test to assess the resistance of Staphylococcus aureus biofilm cells to disinfectants Appl Environ Microbiol 68 4194 4200
15. J Maillard 2007/6 Bacterial resistance to biocides in the healthcare environment: should it be of genuine concern? J Hosp Infect 65 60 72
16. K Marion J Freney G James E Bergeron FNR Renaud JW Costerton 2006 Using an efficient biofilm detaching agent: an essential step for the improvement of endoscope reprocessing protocols J Hosp Infect 64 136 142
17. A Mariscal M Carnero-Varo M Gutierrez-Bedmar A Garcia-Rodriguez J Fernandez-Crehuet 2007 A fluorescent method for assessing the antimicrobial efficacy of disinfectant against Escherichia coli ATCC 35218 biofilm Appl Microbiol Biotechnol 77 233 240
18. J O'Brien I Wilson T Orton F Pognan 2000 Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity Eur J Biochem 267 5421 5426
19. GA O'Toole R Kolter 1998 Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: a genetic analysis Mol Microbiol 28 449 461
20. E Peeters HJ Nelis T Coenye 2008 Comparison of multiple methods for quantification of microbial biofilms grown in microtiter plates J Microbiol Methods 72 157 165
21. E Peeters HJ Nelis T Coenye 2008 Evaluation of the efficacy of disinfection procedures against Burkholderia cenocepacia biofilms J Hosp Infect 70 361 368 doi:10.1016/j.jhin.2008.08.015
22. C Perret-Vivancos K Marion FNR Renaud J Freney 2008 Efficient removal of attached biofilm in a naturally contaminated colonoscope using detachment-promoting agents J Hosp Infect 68 277 278
23. B Pitts MA Hamilton N Zelver PS Stewart 2003 A microtiter-plate screening method for biofilm disinfection and removal J Microbiol Methods 54 269 276
24. G Reid 1999 Biofilms in infectious disease and on medical devices Int J Antimicrob Agents 11 223 226
25. SD Sarker L Nahar Y Kumarasamy 2007 Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals Methods 42 321 324
26. Z Shi KG Neoh ET Kang W Wang 2006 Antibacterial and mechanical properties of bone cement impregnated with chitosan nanoparticles Biomaterials 27 2440 2449
27. M Shiloh J Ruan C Nathan 1997 Evaluation of bacterial survival and phagocyte function with a fluorescence-based microplate assay Infect Immun 65 3193 3198
28. R Singh D Paul RK Jain 2006 Biofilms: implications in bioremediation Trends Microbiol 14 389 397 doi:10.1016/j.tim.2006.07.001
29. S Stepanovic D Vukovic I Dakic B Savic M Svabic-Vlahovic 2000 A modified microtiter-plate test for quantification of staphylococcal biofilm formation J Microbiol Methods 40 175 179
30. N Surdeau D Laurent-Maquin S Bouthors MP Gelle 2006/4 Sensitivity of bacterial biofilms and planktonic cells to a new antimicrobial agent, Oxsil(R) 320N J Hosp Infect 62 487 493
31. AC Tizzard JH Bergsma G Lloyd-Jones 2006 A resazurin-based biosensor for organic pollutants Biosens Bioelectron 22 759 763
32. RS Twigg 1945 Oxidation-reduction aspects of Resazurin Nature 155 401 402
33. E van der Wende WG Characklis DB Smith 1989 Biofilms and bacterial drinking water quality Water Res 23 1313 1322
34. S Yamashoji A Asakawa S Kawasaki S Kawamoto 2004 Chemiluminescent assay for detection of viable microorganisms Anal Biochem 333 303 308
35. N Zelver M Hamilton D Goeres J Heersink 2001 Development of a standardized antibiofilm test Methods Enzymol 337 363 376