Thiamin biosynthesis and its influence on exopolysaccharide production: A new component of virulence identified on Erwinia amylovora plasmid pEA29

Acta Horticulturae

Volumn 793, Issue , 2008, Pages 271-278

  McGhee, G C ^a   Sundin, G W ^a  

^a MICHIGAN STATE UNIVERSITY   (United States)

Author keywords

Ams operon; Amylovoran; GUS reporter; Transcriptional fusions

Indexed keywords

ERWINIA AMYLOVORA; MALUS X DOMESTICA; PYRUS;

EID: 61449144708     PISSN: 05677572     EISSN: None     Source Type: Book Series    
DOI: 10.17660/actahortic.2008.793.40     Document Type: Conference Paper

References (13)

1. Blatny, J.M., Brautaset, T., Winther-Larsen, H.C., Haugan, K. and Valla, S. 1997. Construction and use of a versatile set of broad-host-range cloning and expression vectors based on the RK2 replicon. Appl. Environ. Microbiol. 63:370-379.
2. Bradford, M.M. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 72:248-254.
3. Bugert, P. and Geider, K. 1995. Molecular analysis of the ams operon required for exopolysaccharide synthesis of Erwinia amylovora. Mol. Microbiol. 15(5):917-933.
4. Chiou, C.S. and Jones, A.L. 1993. Nucleotide sequence analysis of a transposon (Tn5393) carrying streptomycin resistance genes in Erwinia amylovora and other gram-negative bacteria. J. Bacteriol. 175(3):732-740.
5. Churchward, G., Belin, D. and Nagamine, Y. 1984. A pSC101-derived plasmid which shows no sequence homology to other commonly used cloning vectors. Gene 31:165-71.
6. Falkenstein, H., Bellemann, H., Walter, S., Zeller, W. and Geider, K. 1988. Identification of Erwinia amylovora, the fireblight pathogen, by colony hybridization with DNA from plasmid pEA29. Appl. Environ. Microbiol. 54:2798-2802.
7. Jefferson, R.A. 1987. Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol. Biol. Rep. 5:387-405.
8. Laurent, J., Barny, J., Katoujansky, A., Dufriche, P. and Vanneste, J.L. 1989. Characterization of a ubiquitous plasmid in Erwinia amylovora. Mol. Plant-Microbe Interact. 2:160-164.
9. McGhee, G.C. and Jones, A.L. 2000. Complete nucleotide sequences of ubiquitous plasmid pEA29 from Erwinia amylovora strain Ea88: gene organization and intraspecies variation. Appl. Environ. Microbiol. 66:4897-4907.
10. Ritchie, D.F. and Klos, E.J. 1977. Isolation of Erwinia amylovora bacteriophage from the aerial parts of apple trees. Phytopathology 67:101-104.
11. Sambrook, J. and Russell, D.W. 2001. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press. Cold Spring Harbor, N.Y.
12. Wilson, K.J., Sessitsch, A., Corbo, J.C., Giller, K.E., Akkermans, A.D.L. and Jefferson, R.A. 1995. Glucuronidase (GUS) transposons for ecological and genetic studies of rhizobia and other Gram-negative bacteria. Microbiology 141:1691-1705.
13. Zhao, Y., Blumer, S.E. and Sundin, G.W. 2005. Identification of Erwinia amylovora genes induced during infection of immature pear tissue. J. Bacteriol. 187(23):8808-8103.