Bacillus sphaericus Mtx1 and Mtx2 toxins co-expressed in Escherichia coli are synergistic against Aedes aegypti larvae

Biotechnology Letters

Volumn 31, Issue 4, 2009, Pages 551-555

  Rungrod, Amporn ^a   Tjahaja, Natalia Kristianti ^a   Soonsanga, Sumarin ^a   Audtho, Mongkon ^a   Promdonkoy, Boonhiang ^a  

^a NATIONAL SCIENCE AND TECHNOLOGY DEVELOPMENT AGENCY   (Thailand)

Author keywords

Aedes aegypti; Bacillus sphaericus; Co expression; Fusion protein; Mosquitocidal toxin; Synergism

Indexed keywords

AEDES AEGYPTI; BACILLUS SPHAERICUS; CO-EXPRESSION; FUSION PROTEIN; MOSQUITOCIDAL TOXIN; SYNERGISM;

ANTIGENS; BACTERIOLOGY; ESCHERICHIA COLI; MOSQUITO CONTROL; PEST CONTROL; TOXICITY;

METABOLITES;

BACTERIAL PROTEIN; BACTERIAL TOXIN; INSECTICIDE; MTX1 PROTEIN, BACILLUS SPHAERICUS; MTX2 PROTEIN, BACILLUS SPHAERICUS;

AEDES; ANIMAL; ARTICLE; BIOSYNTHESIS; DRUG EFFECT; DRUG POTENTIATION; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; LARVA; LD 50;

AEDES; ANIMALS; BACTERIAL PROTEINS; BACTERIAL TOXINS; DRUG SYNERGISM; ESCHERICHIA COLI; INSECTICIDES; LARVA; LETHAL DOSE 50;

AEDES AEGYPTI; BACILLUS SPHAERICUS; BACTERIA (MICROORGANISMS); CULEX PIPIENS QUINQUEFASCIATUS; ESCHERICHIA COLI;

EID: 61449109945     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-008-9896-x     Document Type: Article

References (15)

1. I Carpusca T Jank K Aktories 2006 Bacillus sphaericus mosquitocidal toxin (MTX) and pierisin: the enigmatic offspring from the family of ADP-ribosyltransferases Mol Microbiol 62 621 630
2. SW Chan T Thanabalu BY Wee AG Porter 1996 Unusual amino acid determinants of host range in the Mtx2 family of mosquitocidal toxins J Biol Chem 271 14183 14187
3. JF Charles C Nielson-LeRoux A Delecluse 1996 Bacillus sphaericus toxins: molecular biology and mode of action Annu Rev Entomol 41 451 472
4. Finney D (1971) Probit analysis. Cambridge University Press, London
5. JW Liu AG Porter BY Wee T Thanabalu 1996 New gene from nine Bacillus sphaericus strains encoding highly conserved 35.8-kilodalton mosquitocidal toxins Appl Environ Microbiol 62 2174 2176
6. B Promdonkoy P Promdonkoy M Audtho S Tanapongpipat N Chewawiwat P Luxananil S Panyim 2003 Efficient expression of the mosquito larvicidal binary toxin gene from Bacillus sphaericus in Escherichia coli Curr Microbiol 47 383 387
7. B Promdonkoy P Promdonkoy S Tanapongpipat P Luxananil N Chewawiwat M Audtho S Panyim 2004 Cloning and characterization of a mosquito larvicidal toxin produced during vegetative stage of Bacillus sphaericus 2297 Curr Microbiol 49 84 88
8. DJ Reinert I Carpusca K Aktories GE Schulz 2006 Structure of the mosquitocidal toxin from Bacillus sphaericus J Mol Biol 357 1226 1236
9. J Schirmer I Just K Aktories 2002 The ADP-ribosylating mosquitocidal toxin from Bacillus sphaericus: proteolytic activation, enzyme activity, and cytotoxic effects J Biol Chem 277 11941 11948
10. T Thanabalu AG Porter 1995 Efficient expression of a 100-kilodalton mosquitocidal toxin in protease-deficient recombinant Bacillus sphaericus Appl Environ Microbiol 61 4031 4036
11. T Thanabalu AG Porter 1996 A Bacillus sphaericus gene encoding a novel type of mosquitocidal toxin of 31.8 kDa Gene 170 85 89
12. T Thanabalu C Berry J Hindley 1993 Cytotoxicity and ADP-ribosylating activity of the mosquitocidal toxin from Bacillus sphaericus SSII-1: possible roles of the 27- and 70-kilodalton peptides J Bacteriol 175 2314 2320
13. T Thanabalu J Hindley J Jackson-Yap C Berry 1991 Cloning, sequencing, and expression of a gene encoding a 100-kilodalton mosquitocidal toxin from Bacillus sphaericus SSII-1 J Bacteriol 173 2776 2785
14. MC Wirth Y Yang WE Walton BA Federici C Berry 2007 Mtx toxins synergize Bacillus sphaericus and Cry11Aa against susceptible and insecticide-resistant Culex quinquefasciatus larvae Appl Environ Microbiol 73 6066 6071
15. Y Yang L Wang A Gaviria Z Yuan C Berry 2007 Proteolytic stability of insecticidal toxins expressed in recombinant bacilli Appl Environ Microbiol 73 218 225