Multicontrast microscopy technique to dynamically fingerprint live-cell focal contacts during exposure and replacement of a cytotoxic medium

Journal of Biomedical Optics

Volumn 13, Issue 5, 2008, Pages

  Choi, Chang K ^a   Margraves, Charles H ^a   English, Anthony E ^a   Kihm, Kenneth D ^a  

^a University of Tennessee Knoxville   (United States)

Author keywords

cytotoxicity; dynamic cellular fingerprinting; endothelial cells; focal contacts; interference reflection contrast microscopy

Indexed keywords

CONTACT AREAS; CYTOTOXIC; CYTOTOXIC AGENTS; DIFFERENTIAL INTERFERENCE CONTRAST MICROSCOPY; FOCAL CONTACTS; HIGH DOSE; INTERFERENCE REFLECTION; INTERFERENCE REFLECTION CONTRAST MICROSCOPY; MICROSCOPY TECHNIQUE; MORPHOLOGICAL RESPONSE; NATURAL FRINGE PATTERNS; PULMONARY ARTERY ENDOTHELIAL CELLS; TOXIC AGENTS;

CYTOTOXICITY; ENDOTHELIAL CELLS;

FOCUSING;

SUS;

CYTOCHALASIN D; CYTOTOXIN;

ANIMAL; ARTICLE; CELL ADHESION; CELL CULTURE; CYTOLOGY; DRUG EFFECT; EPITHELIUM CELL; IMAGE ENHANCEMENT; METHODOLOGY; PHASE CONTRAST MICROSCOPY; PHYSIOLOGY; SWINE;

ANIMALS; CELL ADHESION; CELLS, CULTURED; CYTOCHALASIN D; CYTOTOXINS; EPITHELIAL CELLS; IMAGE ENHANCEMENT; MICROSCOPY, PHASE-CONTRAST; SWINE;

EID: 60849088285     PISSN: 10833668     EISSN: 15602281     Source Type: Journal    
DOI: 10.1117/1.2993143     Document Type: Article

References (7)

1. A. S. G., Curtis, " The mechanism of adhesion of cells to glass, ", J. Cell Biol., 20, 199-215 (1964). jla JCLBA3 0021-9525 10.1083/jcb.20.2.199
2. C. S., Izzard and L. R., Lochner, " Cell-to-substrate contacts in living fibroblasts: an interference reflexion study with an evaluation of the technique, ", J. Cell. Sci., 21, 129-159 (1976). jcb JNCSAI 0021-9533
3. D., Gingell and I., Todd, " Interference reflection microscopy: a quantitative theory for image interpretation and its application to cell-substratum separation measurement, ", Biophys. J., 26, 507-526 (1979). bio BIOJAU 0006-3495 (Pubitemid 9205705)
4. C. K., Choi, A. E., English, K. D., Kihm, and C. H., Margraves, " Simultaneous dynamic optical and electrical properties of endothelial cell attachment on indium tin oxide bioelectrodes, ", J. Biomed. Opt., 12 (6), 064028 (2007). jbo JBOPFO 1083-3668 10.1117/1.2821407
5. H., Verschueren, " Interference reflection microscopy in cell biology: methodology and applications, ", J. Cell. Sci., 75, 279-301 (1985). jcb JNCSAI 0021-9533
6. M., Mansuripur, Classical Optics and Its Applications, Cambridge University Press, New York (2002).
7. E., Hecht, Optics, 4th ed., Addison Wesley, New York (2002).