Expression of YeeK during Bacillus subtilis sporulation and localization of YeeK to the inner spore coat using fluorescence microscopy

Journal of Bacteriology

Volumn 191, Issue 4, 2009, Pages 1220-1229

  Takamatsu, Hiromu ^a   Imamura, Daisuke ^a   Kuwana, Ritsuko ^a   Watabe, Kazuhito ^a  

^a SETSUNAN UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; COTE PROTEIN; GERE PROTEIN; GREEN FLUORESCENT PROTEIN; MUTANT PROTEIN; RED FLUORESCENT PROTEIN; RNA POLYMERASE; SAFA PROTEIN; SIGK CONTAINING RNA POLYMERASE; SPOVID PROTEIN; UNCLASSIFIED DRUG; YABG PROTEIN; YEEK PROTEIN;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL SPORE; FLUORESCENCE MICROSCOPY; GENE DISRUPTION; GENE EXPRESSION; IMMUNOBLOTTING; INNER SPORE COAT; NONHUMAN; NORTHERN BLOTTING; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DEGRADATION; PROTEIN LOCALIZATION; SPORE GERMINATION; SPORE RESISTANCE; SPOROGENESIS; VEGETATIVE GROWTH; WILD TYPE; YABG GENE; YEEK GENE; CHEMISTRY; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; PHYSIOLOGY; PROTEIN TRANSPORT;

BACILLUS SUBTILIS;

BACILLUS SUBTILIS; BACTERIAL PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; MICROSCOPY, FLUORESCENCE; PROTEIN TRANSPORT; SPORES, BACTERIAL;

EID: 60849084557     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.01269-08     Document Type: Article

References (56)

1. Aronson, A. I., H. Y. Song, and N. Bourne. 1989. Gene structure and precursor processing of a novel Bacillus subtilis spore coat protein. Mol. Microbiol. 3:437-444.
2. Aronson, A. I., and P. Fitz-James. 1976. Structure and morphogenesis of the bacterial spore coat. Bacteriol. Rev. 40:360-402.
3. Bagyan, L, M. Noback, S. Bron, M. Paidhungat, and P. Setlow. 1998. Characterization of yhcN, a new fore spore-specific gene of Bacillus subtilis, Gene 212:179-188.
4. Bauer, T., S. Little, A. G. Stover, and A. Driks. 1999. Functional regions of the Bacillus subtilis spore coat morphogenetic protein CotE. J. Bacteriol. 181:7043-7051.
5. Beall, B., A. Driks, R. Losick, and C. P. Moran Jr. 1993. Cloning and characterization of a gene required for assembly of the Bacillus subtilis spore coat. J. Bacteriol. 175:1705-1716.
6. Bourne, N., P. C. Fitz-James, and A. I. Aronson. 1991. Structural and germination defects of Bacillus subtilis spores with altered contents of a spore coat protein. J. Bacteriol. 173:6618-6625.
7. Catalano, F. A., J. Meador-Parton, D. L. Popham, and A. Driks. 2001. Amino acids in the Bacillus subtilis morphogenetic protein SpoIVA with roles in spore coat and cortex formation. J. Bacteriol. 183:1645-1654.
8. Costa, T., A. L. Isidro, C. P. Moran, Jr., and A. O. Henriques. 2006. Interaction between coat morphogenetic proteins Saf A and SpoVID. J. Bacteriol. 188:7731-7741.
9. Cutting, S., L. B. Zheng, and R. Losick. 1991. Gene encoding two alkalisoluble components of the spore coat from Bacillus subtilis. J. Bacteriol. 173:2915-2919.
10. Cutting, S., and P. B. Vander Horn. 1990. Genetic analysis, p. 27-74. In C. R. Harwood, and S. M. Cutting (ed.). Molecular biological methods for Bacillus. John Wiley & Sons. Chichester, England.
11. Driks, A. 2003. The dynamic spore. Proc. Natl. Acad. Sci. USA 100:3007-3009.
12. Driks, A. 2002. Proteins of the spore core and cortex, p. 527-535. In A. Sonenshein et al. (ed.), Bacillus subtilis and its closest relatives from genes to cells. ASM Press, Washington, DC.
13. Driks, A. 2002. Maximum shields: the assembly and function of the bacterial spore coat. Trends Microbiol. 10:251-254.
14. Driks, A. 1999. Bacillus subtilis spore coat. Microbiol. Mol. Biol. Rev. 63:1-20.
15. Driks, A., S. Roels, B. Beall, C. P. Moran, Jr., and R. Losick. 1994. Subcellular localization of proteins involved in the assembly of the spore coat of Bacillus subtilis. Genes Dev. 8:234-244.
16. Ducros, V. M.-A., R. J. Lewis, C. S. Verma, E. J. Dodson, G. Leonard, J. P. Turkenburg, G. N. Murshudov, A. J. Wilkinson, and J. A. Brannigan. 2001. Crystal structure of GerE, the ultimate transcriptional regulator of spore formation in Bacillus subtilis. J. Mol. Biol. 306:759-771.
17. Errington, J. 1990. Gene cloning techniques, p. 175-220. In C. R. Harwood, and S. M. Cutting (ed.), Molecular biological methods for Bacillus. John Wiley and Sons, Chichester, England.
18. Fawcett, P., P. Eichenberger, R. Losick, and P. Youngman. 2000. The transcriptional profile of early to middle sporulation in Bacillus subtilis. Proc. Natl. Acad. Sci. USA 97:8063-8068.
19. Henriques, A. O., and C. P. Moran, Jr. 2007. Structure, assembly, and function of the spore surface layers. Annu. Rev. Microbiol. 61:555-588.
20. Herrin, D. L., and G. W. Schmidt. 1988. Rapid, reversible staining of northern blots prior to hybridization. BioTechniques 6:196-. 197:199-200.
21. Igo, M. M., and R. Losick. 1986. Regulation of a promoter that is utilized by minor forms of RNA polymerase holoenzyme in Bacillus subtilis. J. Mol. Biol. 191:615-624.
22. Isticato, R., A. Pelosi, R. Zilhao, L. Baccigalupi, A. O. Henriques, M. D. Felice, and E. Ricca. 2007. CotC-CotU heterodimerization during assembly of the Bacillus subtilis spore coat. J. Bacteriol. 190:1267-1275.
23. Kim, H., M. Hahn, P. Grabowski, D. C. McPherson, M. W. Otte, R. Wang, C. C. Ferguson, P. Eichenberger, and A. Driks. 2006. The Bacillus subtilis spore coat protein interaction network. Mol. Microbiol. 59:487-502.
24. Klobutcher, L. A., K. Ragkousi, and P. Setlow. 2006. The Bacillus subtilis spore coat provides "eat resistance" during phagocytic predation by the protozoan Tetrahymena thermophila. Proc. Natl. Acad. Sci. USA 103:165-170.
25. Kodama, T., H. Takamatsu, K. Asai, K. Kobayashi, N. Ogasawara, and K. Watabe. 1999. The Bacillus subtilis yaaH gene is transcribed by SigE RNA polymerase during sporulation, and its product is involved in germination of spores. J. Bacteriol. 181:4584-4591.
26. Kuwana, R., H. Takamatsu, and K. Watabe. 2007. Expression, localization and modification of YxeE spore coat protein in Bacillus subtilis. J. Biochem. (Tokyo). 142:681-689.
27. Kuwana, R., N. Okuda, H. Takamatsu, and K. Watabe. 2006. Modification of GerQ reveals a functional relationship between Tgl and YabG in the coat of Bacillus subtilis spores. J. Biochem. (Tokyo) 139:887-901.
28. Kuwana, R., H. Ikejiri, S. Yamamura, H. Takamatsu, and K. Watabe. 2004. Functional relationship between SpoVIF and GerE in gene regulation during sporulation of Bacillus subtilis. Microbiology 150:163-170.
29. Kuwana, R., Y. Kasahara, M. Fujibayashi, H. Takamatsu, N. Ogasawara, and K. Watabe. 2002. Proteomics characterization of novel spore proteins of Bacillus subtilis. Microbiology 148:3971-3982.
30. Laaberki, M.-H., and J. Dworkin. 2008. Role of spore coat proteins in the resistance of Bacillus subtilis spores to Caenorhabditis elegans predation. J. Bacteriol. 190:6197-6203.
31. Lai, E.-M., N. D. Phadke, M. T. Kachman, R. Giorno, S. Vazquez, J. A. Vazquez, J, R. Maddock, and A. Driks. 2003. Proteomic analysis of the spore coats of Bacillus subtilis and Bacillus anthracis. J. Bacteriol. 185:1443-1454.
32. Little, S., and A. Driks. 2001. Functional analysis of the Bacillus subtilis morphogenetic spore coat protein CotE. Mol. Microbiol. 42:1107-1120.
33. Moriya, S., E. Tsujikawa, A. K. M. Hassan, K. Asai, T. Kodama, and N. Ogasawara. 1998. A Bacillus subtilis gene-encoding protein homologous to eukaryotic SMC motor protein is necessary for chromosome partition. Mol. Microbiol. 29:179-187.
34. Nicholson, W., N. Munakata, G. Horneck, H. J. Melosh, and P. Setlow. 2000. Resistance of Bacillus endospores to extreme terrestrial and extraterrestrial environments. Microbiol. Mol. Biol. Rev. 64:548-572.
35. Ozin, A. J., A. O. Henriques, H. Yi, and C. P. Moran, Jr. 2000. Morphogenetic proteins SpoVID and SafA form a complex during assembly of the Bacillus subtilis spore coat. J. Bacteriol. 182:1828-1833.
36. Paidhungat, M., and P. Setlow. 2001. Spore germination and outgrowth, p. 537-548. In A. Sonenshein et al. (ed.), Bacillus subtilis and its closest relatives from genes to cells. ASM press, Washington. DC.
37. Piggot, P. J., and R. Losick. 2002. Sporulation genes and intercompartmental regulation, p. 483-517. In A. Sonensheinet al. (ed.), Bacillus subtilis and its closest relatives from genes to cells. ASM press, Washington. DC.
38. Price, K. D., and R. Losick. 1999. A four-dimensional view of assembly of a morphogenetic protein during sporulation in Bacillus subtilis. J. Bacteriol. 181:781-790.
39. Roels, S., and R. Losick. 1995. Adjacent and divergently oriented operons under the control of the sporulation regulatory protein GerE in Bacillus subtilis. J. Bacteriol. 177:6263-6275.
40. Roels, S., A. Driks, and R. Losick. 1992. Characterization of spoIVA, a sporulation gene involved in coat morphogenesis in Bacillus subtilis. J. Bacteriol. 174:575-585.
41. Sacco, M., E. Ricca, R. Losick, and S. Cutting. 1995. An additional GerE-controlled gene encoding an abundant spore coat protein from Bacillus subtilis. J. Bacteriol. 177:372-377.
42. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual. 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
43. Schaeffer, P., J. Millet, and J.-P. Aubert. 1965. Catabolic repression of bacterial sporulation. Proc. Natl. Acad. Sci. USA 54:704-711.
44. Steil, L., M. Serrano, A. O. Henriques, and U. Völker. 2005. Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis. Microbiology 151:399-420.
45. Stevens, C. M., R. Daniel, N. Illing, and J. Errington. 1992. Characterization of a sporulation gene, spoIVA, involved in spore coat morphogenesis in Bacillus subtilis. J. Bacteriol. 174:586-594.
46. Stragier, P., and R. Losick. 1996. Molecular genetics of sporulation in Bacillus subtilis. Annu. Rev. Genet. 30:297-341.
47. Takamatsu, H., and K. Watabe. 2002. Assembly and genetics of spore protective structures. Cell. Mol. Life Sci. 59:434-444.
48. Takamatsu, H., T. Kodama, A. Imamura, K. Asai, K. Kobayashi, T. Nakayama, N. Ogasawara, and K. Watabe. 2000. The Bacillus subtilis yabG gene is transcribed by SigK RNA polymerase during sporulation, and yabG mutant spores have altered coat protein composition. J. Bacteriol. 182:1883-1888.
49. Takamatsu, H., A. Imamura, T. Kodama, K. Asai, N. Ogasawara, and K. Watabe. 2000. The yabG gene of Bacillus subtilis encodes a sporulation specific protease which is involved in the processing of several spore coat proteins. FEMS Microbiol. Lett. 192:33-38.
50. Takamatsu, H., T. Kodama, T. Nakayama, and K. Watabe. 1999. Characterization of the yrbA gene of Bacillus subtilis, involved in resistance and germination of spores. J, Bacteriol. 181:4986-4994.
51. Vagner, V., E. Dervyn, and S. D. Ehrlich. 1998. A vector for systematic gene inactivation in Bacillus subtilis. Microbiology 144:3097-3104.
52. Warth, A. D., D. F. Ohye, and W. G. Murrell. 1963. The composition and structure of bacterial spores. J. Cell Biol. 16:579-592.
53. Webb, C. D., A. Decatur, A. Teleman, and R. Losick. 1995. Use of green fluorescent protein for visualization of cell-specific gene expression and subcellular protein localization during sporulation in Bacillus subtilis. J. Bacteriol. 177:5906-5911.
54. Zheng, L., R. Halberg, S. Roels, H. Ichikawa, L. Kroos, and R. Losick. 1992. Sporulation regulatory protein GerE from Bacillus subtilis binds to and can activate or repress transcription from promoters for mother-cell-specific genes. J. Mol. Biol. 226:1037-1050.
55. Zheng, L. B., and R. Losick. 1990. Cascade regulation of spore coat gene expression in Bacillus subtilis. J. Mol. Biol. 20:645-660.
56. Zheng, L. B., W. P. Donovan, P. C. Fitz-James, and R. Losick. 1988. Gene encoding a morphogenic protein required in the assembly of the outer coat of the Bacillus subtilis endospore. Genes Dev. 2:1047-1054.