Identification of polyploidy of in vitro anther-derived shoots of Asparagus officinalis L. by flow cytometric analysis and measurement of stomatal length

Journal of the Japanese Society for Horticultural Science

Volumn 78, Issue 1, 2009, Pages 103-108

  Shiga, Ikko ^a   Uno, Yuichi ^a   Kanechi, Michio ^a   Inagaki, Noboru ^a  

^a KOBE UNIVERSITY   (Japan)

Author keywords

Anther culture; Asparagus officinalis L; Flow cytometry; Polyploids; Stomata

Indexed keywords

ASPARAGUS; ASPARAGUS OFFICINALIS; PHYLLODES;

EID: 60749132996     PISSN: 18823351     EISSN: None     Source Type: Journal    
DOI: 10.2503/jjshs1.78.103     Document Type: Article

References (18)

1. Amano, J., S. Kuwayama, Y. Mizuta, T. Oomiya, T. Nakamura and M. Nakano. 2007. Early Identification of Intra- and Intergeneric Hybrids among Colchicaceous Ornamentals, Gloriosa spp., Littonia modesta Hook, and Sandersonia aurantiaca Hook., by Flow Cytometry and Random Amplified Polymorphic DNA Analyses. J. Japan. Soc. Hort. Sci. 76: 73-78.
2. Araki, H., H. Shimazaki, Y. Hirata, T. Oridate, T. Harada and T. Yakuwa. 1992. Chromosome number variation of callus cells and regenerated plants in Asparagus officinalis L. Plant Tissue Culture Lett. 9: 169-175.
3. Aryavand, A., B. Ehdaie, B. Tran and J. G. Waines. 2003. Stomatal frequency and size differentiate ploidy levels in Aegilops neglecta. Genet. Resour. Crop Evol. 50: 175-182.
4. Cardi, T., V. Iannamico, F. D'Ambrosio, E. Filippone and P. F. Lurquin. 1993. In vitro regeneration and cytological characterization of shoots from leaf explants of three accessions of Solanum commersonii. Plant Cell Tiss. Org. Cult. 34: 107-114.
5. Carvalho, J. F. R. P., C. R. Carvalho and W. C. Otoni. 2005. In vitro induction of polyploidy in annatto (Bixa orellana). Plant Cell Tiss. Org. Cult. 80: 69-75.
6. Falavigna, A., M. G. Tacconi and P. E. Casali. 1985. Asparagus breeding through anther in vitro culture: Eight years of activity in Italy. Proceeding of the Sixth International Asparagus Symposium: 31-40.
7. th IS on Asparagus. Acta Hort. 589: 201-210.
8. Feng, X. R. and D. J. Wolyn. 1991. High frequency production of haploid embryos in asparagus anther culture. Plant Cell Rep. 10: 574-578.
9. Kadota, M. and Y. Niimi. 2002. In vitro induction of tetraploid plants from a diploid Japanese pear cultivar (Pyrus pyrifolia N. cv. Hosui). Plant Cell Rep. 21: 282-286.
10. Koutoulis, A., T. A. Roy, A. Price, L. Sherriff and G. Leggett. 2005. DNA ploidy level of colchicine-treated hops (Humulus lupulus L.). Sci. Hortic. 105: 263-268.
11. Limanton-Grevet, A., B. Sotta, S. Brown and M. Jullien. 2000. Analysis of habituated embryogenic lines in Asparagus officinalis L.: growth characteristics, hormone content and ploidy level of calli and regenerated plants. Plant Sci. 160: 15-26.
12. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.
13. Nakano, M., T. Nomizu, K. Mizunashi, M. Suzuki, S. Mori, S. Kuwayama, M. Hayashi, H. Umehara, E. Oka, H. Kobayashi, M. Asano, S. Sugawara, H. Takagi, H. Saito, M. Nakata, T. Godo, Y. Hara and J. Amano. 2006. Somaclonal variation in Tricyrtis hirta plants regenerated from 1-year-old embryogenic callus cultures. Sci. Hortic. 110: 366-371.
14. Santos, R. F. and W. Handro. 1983. Morphological patterns in Petunia hybrida plants regenerated from tissue cultures and differing by their ploidy. Theor. Appl. Genet. 66: 55-60.
15. Thiem, B. and E. Sliwinska. 2003. Flow cytometric analysis of nuclear DNA content in cloudberry (Rubus chamaemorus L.) in vitro cultures. Plant Sci. 164: 129-134.
16. th inter. Asparagus Symposium: 22-30.
17. Yamada, A. and R. Tao. 2007. Controlled Pollination with Sorted Reduced and Unreduced Pollen Grains Reveals Unreduced Embryo Sac Formation in Diospyros kaki Thunb. 'Fujiwaragosho'. J. Japan. Soc. Hort. Sci. 76: 133-138.
18. Yang, X. M., Z. Y. Cao, L. Z. An, Y. M. Wang and X. W. Fang. 2006. In vitro tetraploid induction via colchicine treatment from diploid somatic embryos in grapevine (Vitis vinifera L.). Euphytica 152: 217-224.