Validation of a flow cytometry based chemokine internalization assay for use in evaluating the pharmacodynamic response to a receptor antagonist

Journal of Translational Medicine

Volumn 6, Issue , 2008, Pages

  Wyant, Timothy ^a   Lackey, Alan ^b,c   Green, Marie ^a  

^a MILLENNIUM PHARMACEUTICALS INC   (United States)

^b Esoterix Center for Innovation   (United States)

^c Nodality Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHEMOKINE RECEPTOR CCR2 ANTAGONIST; CYTOKINE RECEPTOR ANTAGONIST; MONOCYTE CHEMOTACTIC PROTEIN 1; UNCLASSIFIED DRUG; CHEMOKINE RECEPTOR CCR2; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS;

ANALYTIC METHOD; ARTICLE; CONTROLLED STUDY; DRUG DETERMINATION; DRUG PROTEIN BINDING; DRUG SCREENING; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; INTERNALIZATION; PHARMACODYNAMICS; PROTEIN ANALYSIS; PROTEIN STABILITY; RETROSPECTIVE STUDY; SENSITIVITY AND SPECIFICITY; TITRIMETRY; VALIDATION PROCESS; BIOASSAY; BLOOD CELL; CLINICAL TRIAL; CYTOLOGY; DRUG ANTAGONISM; ENDOCYTOSIS; FLUORESCENCE; FREEZING; IMMUNOLOGICAL MEMORY; IMMUNOLOGY; METABOLISM; METHODOLOGY; MONOCYTE; REPRODUCIBILITY; SOLUBILITY; STAINING; T LYMPHOCYTE; VALIDATION STUDY;

BIOLOGICAL ASSAY; BLOOD CELLS; CHEMOKINE CCL2; ENDOCYTOSIS; FLOW CYTOMETRY; FLUORESCENCE; FREEZING; HUMANS; IMMUNOLOGIC MEMORY; INDICATORS AND REAGENTS; MONOCYTES; RECEPTORS, CCR2; REPRODUCIBILITY OF RESULTS; SOLUBILITY; STAINING AND LABELING; T-LYMPHOCYTES; TITRIMETRY;

EID: 60749110691     PISSN: None     EISSN: 14795876     Source Type: Journal    
DOI: 10.1186/1479-5876-6-76     Document Type: Article

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