Purification Method Using Iodixanol (OptiPrep)-Based Density Gradient Significantly Reduces Cytokine Chemokine Production From Human Islet Preparations, Leading to Prolonged β-Cell Survival During Pretransplantation Culture

Transplantation Proceedings

Volumn 41, Issue 1, 2009, Pages 314-315

  Mita, A ^a,b   Ricordi, C ^a   Miki, A ^a   Barker, S ^a   Khan, A ^a   Alvarez, A ^a   Hashikura, Y ^b   Miyagawa, S ^b   Ichii, H ^a  

^a UNIVERSITY OF MIAMI MILLER SCHOOL OF MEDICINE   (United States)

^b SHINSHU UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CHEMOKINE; CYTOKINE; FICOLL; GAMMA INTERFERON; INTERLEUKIN 1BETA; INTERLEUKIN 6; IODIXANOL; MACROPHAGE INFLAMMATORY PROTEIN 1BETA; TUMOR NECROSIS FACTOR ALPHA;

ARTICLE; CELL COMPOSITION; CELL CULTURE; CELL ISOLATION; CELL SURVIVAL; CELL VIABILITY; CYTOKINE PRODUCTION; DENSITY GRADIENT; FLUORESCENCE ACTIVATED CELL SORTING; HUMAN; HUMAN TISSUE; PANCREAS ISLET BETA CELL; PRIORITY JOURNAL;

ADENOSINE; ALLOPURINOL; AUTOMATION; CELL CULTURE TECHNIQUES; CELL SURVIVAL; CHEMOKINES; CONTRAST MEDIA; CYTOKINES; GLUTATHIONE; HUMANS; INSULIN; INSULIN-SECRETING CELLS; ISLETS OF LANGERHANS; ORGAN PRESERVATION SOLUTIONS; RAFFINOSE; TRIIODOBENZOIC ACIDS;

EID: 60649083628     PISSN: 00411345     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.transproceed.2008.10.059     Document Type: Article

References (6)

1. Ryan E.A., Paty B.W., Senior P.A., et al. Five-year follow-up after clinical islet transplantation. Diabetes 54 (2005) 2060
2. Froud T., Ricordi C., Baidal D.A., et al. Islet transplantation in type 1 diabetes mellitus using cultured islets and steroid-free immunosuppression: Miami experience. Am J Transplant 5 (2005) 2037
3. Hering B.J., Kandaswamy R., Ansite J.D., et al. Single-donor, marginal-dose islet transplantation in patients with type 1 diabetes. JAMA 293 (2005) 830
4. Matsumoto S., Okitsu T., Iwanaga Y., et al. Successful islet transplantation from non-heart-beating donor pancreata using modified Ricordi islet isolation method. Transplantation 82 (2006) 460
5. Ricordi C., Lacy P.E., Finke E.H., et al. Automated method for isolation of human pancreatic islets. Diabetes 37 (1988) 413
6. Ichii H., Inverardi L., Pileggi A., et al. A novel method for the assessment of cellular composition and beta-cell viability in human islet preparations. Am J Transplant 5 (2005) 1635