Measurement of activity of collagenolytic MMP and inhibitors of MMPs using radiolabeled collagen substrate

Nature Protocols

Volumn 4, Issue 3, 2009, Pages 286-290

  Cawston, Tim E ^a  

^a NEWCASTLE UNIVERSITY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

COLLAGEN; ENZYME PRECURSOR; MATRIX METALLOPROTEINASE; MATRIX METALLOPROTEINASE INHIBITOR;

ARTICLE; CENTRIFUGATION; COLLAGEN DEGRADATION; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME SPECIFICITY; ENZYME SUBSTRATE; IMMUNOASSAY; INCUBATION TEMPERATURE; ISOTOPE LABELING; PRIORITY JOURNAL; PROTEIN PURIFICATION; REPRODUCIBILITY; SCINTILLATION COUNTING;

ANIMALS; CATTLE; COLLAGEN TYPE I; ISOTOPE LABELING; METALLOPROTEASES; SKIN; SUBSTRATE SPECIFICITY;

EID: 60549113933     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2008.244     Document Type: Article

References (15)

1. Rowan, A.D. & Young, D.A. Collagenase gene regulation by pro-inflammatory cytokines in cartilage. Front. Biosci. 12, 536-550 (2007).
2. Gisslow, M.T. & McBride, B.C. A rapid sensitive collagenase assay. Anal. Biochem. 68, 70-78 (1975).
3. 14C]acetylated collagen. Anal. Biochem. 99, 340-345 (1979).
4. Terato, K., Nagai, Y., Kawaninski, K. & Shinro, Y. A rapid assay method of collagenase activity using 14C-labelled soluble collagen as substrate. Biochim. Biophys Acta. 445, 753-762 (1976).
5. Johnson-Wint, B. & Gross, J. A quantitative collagen film collagenase assay for large numbers of samples. Anal. Biochem. 104, 175-181 (1980).
6. Cawston, T.E. et al. The role of oncostatin M in animal and human connective tissue turnover and its localisation within the rheumatoid joint. Arthritis Rheum. 41, 1760-1771 (1998).
7. Milner, J.M., Elliott, S.F. & Cawston, T.E. Activation of procollagenase is a key control point in cartilage collagen degradation: Interaction of serine and metalloproteinase pathways. Arthritis Rheum. 44, 2084-2096 (2001).
8. Clark, I.M., Powell, L.K., Wright, J.K., Cawston, T.E. & Hazleman, B.L. Monoclonal antibodies against human fibroblast collagenase and the design of an enzyme-linked immunosorbent assay to measure total collagenase. Matrix 12, 475-480 (1992).
9. Clark, I.M., Powell, L.K., Ramsey, S., Hazleman, B.L. & Cawston, T.E. The measurement of collagenase, tissue inhibitor of metalloproteinases (TIMP) and collagenase-TIMP complex in synovial fluids from patients with osteoarthritis and rheumatoid arthritis. Arthritis Rheum. 36, 372-379 (1993).
10. Harris, E.D. & Vater, C.A. Vertebrate collagenases. Methods Enzymol. 82, 423-458 (1982).
11. Cawston, T.E. & Murphy, G. Mammalian collagenases. Methods Enzymol. 80, 711-722 (1981).
12. Dioszegi, M., Cannon, P. & VanWart, H.E. Vertebrate collagenases. Methods Enzymol. 248, 413-431 (1995).
13. Lombard, C., Saulnier, J. & Wallach, J. Assays of matrixmetalloproteinases (MMPs) activities: A review. Biochimie 87, 265-272 (2005).
14. Hawkes, S.P., Li, H. & Taniguchi, G.T. Zymography and reverse zymography for detecting MMPs and TIMPs. Methods Mol. Biol. 151, 399-410 (2001).
15. Fields, G.B. Using flourogenic peptide substrates to assay matrix metalloproteinases. Methods Mol. Biol. 151, 495-518 (2001).