High-level expression and single-step purification of recombinant Bacillus anthracis protective antigen from Escherichia coli

Biotechnology and Applied Biochemistry

Volumn 52, Issue 2, 2009, Pages 107-112

  Lu, Jinbiao ^a   Wei, Dong ^b   Wang, Yefu ^a   Wang, Guozhi ^b  

^a WUHAN UNIVERSITY   (China)

^b NATIONAL INSTITUTE FOR THE CONTROL OF PHARMACEUTICAL AND BIOLOGICAL PRODUCTS   (China)

Author keywords

Anthrax; Bacillus anthracis; Escherichia coli strain Rosetta 2(DE3); Hydrophobic interaction chromatography (HIC); Protective antigen (PA); Single step purification

Indexed keywords

ANTHRAX; BACILLUS ANTHRACIS; HYDROPHOBIC-INTERACTION CHROMATOGRAPHY (HIC); PROTECTIVE ANTIGEN (PA); SINGLE-STEP PURIFICATION;

AMMONIUM COMPOUNDS; ANTIGENS; BACTERIOLOGY; CHROMATOGRAPHIC ANALYSIS; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; HYDROPHOBICITY; METABOLISM; PURIFICATION; RESINS; UREA;

HYDROPHOBIC CHROMATOGRAPHY;

AMMONIUM SULFATE; PROTECTIVE ANTIGEN; RECOMBINANT ANTIGEN; SEPHAROSE; TRITON X 100; UNCLASSIFIED DRUG; UREA; ANTHRAX TOXIN; BACTERIAL ANTIGEN; BACTERIAL TOXIN; RECOMBINANT PROTEIN;

ANTIGEN EXPRESSION; ANTIGEN PURIFICATION; ARTICLE; CELL INCLUSION; CENTRIFUGATION; CHROMATOGRAPHY; CODON; ESCHERICHIA COLI; HIGH SPEED CENTRIFUGATION; METHODOLOGY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRECIPITATION; ANIMAL; BACILLUS ANTHRACIS; BIOSYNTHESIS; CELL LINE; CHEMISTRY; CYTOLOGY; GENE EXPRESSION; GENETICS; HYDROPHOBICITY; IMMUNOLOGY; ISOLATION AND PURIFICATION; MOUSE; TIME;

BACILLUS ANTHRACIS; ESCHERICHIA COLI;

ANIMALS; ANTIGENS, BACTERIAL; BACILLUS ANTHRACIS; BACTERIAL TOXINS; CELL LINE; CHROMATOGRAPHY; ESCHERICHIA COLI; GENE EXPRESSION; HYDROPHOBICITY; INCLUSION BODIES; MICE; RECOMBINANT PROTEINS; TIME FACTORS;

EID: 60549102139     PISSN: 08854513     EISSN: None     Source Type: Journal    
DOI: 10.1042/BA20070245     Document Type: Article

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