A direct spectrophotometric γ-glutamyltransferase inhibitor screening assay targeting the hydrolysis-only mode

Biochemical and Biophysical Research Communications

Volumn 380, Issue 3, 2009, Pages 591-596

  Vergauwen, Bjorn ^a   Dudycz, Lech W ^b   Dansercoer, Ann ^a   Devreese, Bart ^a  

^a GHENT UNIVERSITY   (Belgium)

^b INFICHEM   (United States)

Author keywords

Phenylthio peptide derivative; Glutamyl transpeptidase; Glutamyltransferase; Continuous assay; Ellman's reagent; Enzyme kinetics; Glutathione; High throughput inhibitor screening; Thiophenol

Indexed keywords

5,5' DITHIOBIS(2 NITROBENZOIC ACID); CANFOSFAMIDE; ENZYME INHIBITOR; GAMMA GLUTAMYLTRANSFERASE; GLUTATHIONE; THIOPHENOL;

ANIMAL EXPERIMENT; ARTICLE; CATALYSIS; COLORIMETRY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME MECHANISM; ENZYME SYNTHESIS; GAMMA GLUTAMYLTRANSFERASE ASSAY; HIGH THROUGHPUT SCREENING; HYDROLYSIS; MOUSE; NONHUMAN; PRIORITY JOURNAL; SPECTROPHOTOMETRY;

ANIMALS; CATALYSIS; COLORIMETRY; DRUG EVALUATION, PRECLINICAL; ENZYME INHIBITORS; GAMMA-GLUTAMYLTRANSFERASE; HUMANS; HYDROLYSIS; MICE; MICE, INBRED C57BL; MOLECULAR MIMICRY; OLIGOPEPTIDES; SPECTROPHOTOMETRY;

EQUIDAE; MAMMALIA;

EID: 60549083463     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2009.01.129     Document Type: Article

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