High-throughput lensfree imaging and characterization of a heterogeneous cell solution on a chip

Biotechnology and Bioengineering

Volumn 102, Issue 3, 2009, Pages 856-868

  Su, Ting Wei ^a   Seo, Sungkyu ^a   Erlinger, Anthony ^a   Ozcan, Aydogan ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Cell counting; Global health; High throughput cytometry; Lensfree lensless imaging; On chip imaging; Point of care devices

Indexed keywords

CELL COUNTING; GLOBAL HEALTH; HIGH-THROUGHPUT CYTOMETRY; LENSFREE-LENSLESS IMAGING; ON-CHIP IMAGING; POINT-OF-CARE DEVICES;

BIOASSAY; BIOCHIPS; BLOOD; CELL CULTURE; DIFFRACTION; HEALTH; HOLOGRAPHIC INTERFEROMETRY; IMAGE SENSORS; MEDICAL PROBLEMS; MICROARRAYS; SCANNING; THREE DIMENSIONAL; THROUGHPUT;

MEDICAL IMAGING;

ALGORITHM; ANIMAL CELL; ARTICLE; BIOSENSOR; CELL COUNT; CELL HETEROGENEITY; CELL TYPE; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; CYTOMETRY; DIFFRACTION; EMBRYO; EMBRYONIC STEM CELL; ERYTHROCYTE; EXPERIMENTAL TEST; FIBROBLAST; HIGH THROUGHPUT LENSFREE IMAGING; IMAGING; LIVER CELL; MEMBRANE MICROPARTICLE; MOUSE; NONHUMAN;

3T3 CELLS; ALGORITHMS; ANIMALS; DATABASES AS TOPIC; DECISION TREES; ELECTRONICS, MEDICAL; ERYTHROCYTES; IMAGE CYTOMETRY; IMAGE PROCESSING, COMPUTER-ASSISTED; MICE; MICROCHIP ANALYTICAL PROCEDURES; MICROSPHERES; OPTICS AND PHOTONICS; POINT-OF-CARE SYSTEMS; SCHIZOSACCHAROMYCES;

EID: 60349120318     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22116     Document Type: Article

References (22)

1. Burger D, Gershman R. 1988. Acoustooptic laser scanning cytometer. Cytometry 9:101-110.
2. Cheng X, Irimia D, Dixon M, Ziperstein JC, Demirce U, Zamir L, Tompkins RG, Toner M, Rodriguez WR. 2005. A microchip approach for practical
3. label-free CD4+ T-Cell counting of HIV-Infected subjects in resource- poor settings. J. Acquir Immune Defic Syndr 45:257-261.
4. Cheng X, Irimia D, Dixon M, Ziperstein JC, Demirce U, Zamir L, Tompkins RG, Rodriguez W, Toner M. 2007. A microfluidic device for practical label-free CD4+ T cell counting of HIV-infected subjects. Lab Chip 7:170-178.
5. Chin CD, Linder V, Sia SK. 2006. Lab-on-a-chip devices for global health: Past studies and future opportunities. Lab Chip 7:41 -57.
6. Darzynkiewicz Z, Elzbieta B, Li X, Gorczyca W, Myron Melamed MR. 1999. Laser-scanning cytometry: A new instrumentation with many applications. Exp Cell Res 249:1-12.
7. Hulett HR, Bonner WA, Sweet RG, Herzenberg LA. 1973. Development and application of a rapid cell sorter. Clin Chem 19:813-816.
8. Joo S, Chung TD, Kim HC. 2007. A rapid field-free electroosmotic micro-pump incorporating charged microchannel surfaces. Sens Actuators B Chem 132:1161-1168.
9. Kamei T, Paegel BM, Scherer JR, Skelley AM, Street RA, Mathies RA. 2003. Integrated hydrogenated amorphous Si photodiode detector for micro-fluidic bioanalytical devices. Anal Chem 75:5300-5305.
10. Kamentsky LA, Burger DE, Gershman RJ, Kamentsky LD, Luther E. 1997.Slide-based laser scanning cytometry. Acta Cytol 41:123-143.
11. Lancaster C, Kokoris A, Nabavi M, Clemmens J, Maloney P, Capadanno J, Gerdes J, Battrell CF. 2005. Rare cancer cell analyzer for whole blood applications: Microcytometer cell counting and sorting subcircuits. Methods 37:120-127.
12. Lee JS. 1981. Speckle analysis and smoothing of synthetic aperture radar images. Comput Graph Image Process 17:24-32.
13. Lee JW. 2003. Global health improvement and WHO: Shaping the future. Lancet 362:2083-2088.
14. Lien V, Zhao K, Berdichevsky Y, Lo YH. 2005. High-sensitivity cytometric detection using fluidic-photonic integrated circuits with array waveguides. IEEE J Sel Top Quantum Electron 11:827-834.
15. Macnab RM, Koshland DE. 1972. The gradient-sensing mechanism in bacterial chemotaxis. Proc Natl Acad Sci 69:2509-2512.
16. Niehren S, Kinzelbach W, Seeger S, Wolfrum J. 1995. An all-solid-state flow cytometer for counting fluorescent microspheres. Anal Chem 67:2666-2671.
17. Novak L, Neuzil P, Pipper J, Zhang Y, Lee SH. 2007. An integrated fluorescence detection system for lab-on-a-chip applications. Lab Chip 6:27-29.
18. Ozcan A, Bilenca A, Bouma BE, Tearney GJ. 2006. Mirror tunnel microscope. Appl Phys Lett 89:131124.
19. Ozcan A, Demirci U. 2007. Ultra wide-field lens-free monitoring of cells on-chip. Lab Chip 8:98-106.
20. Steinberg DM, Ali SZ. 2001. Application of virtual microscopy in clinical cytopathology. Diagn Cytopathol 25:389-396.
21. Weinstein R, Descour M, Liang C, Barker G, Scott K, Richter L, Krupinski E, Bhattacharyya A, Davis J, Graham A. 2004. An array microscope for ultra-rapid virtual slide processing and telepathology. Design, fabrication, and validation study. Hum Pathol 35:1303-1314.
22. Wolff A, Nielsen P, Larsen UD, Friis P, Goranovic G, Poulsen CR, Kutter JP, Telleman P. 2003. Integrating advanced functionality in a microfab- ricated high-throughput fluorescent-activated cell sorter. Lab Chip 3:22-27.