Binding assays for abscisic acid receptors

Methods in Molecular Biology

Volumn 495, Issue , 2009, Pages 89-99

  Razem, Fawzi A ^a   Hill, Robert D ^a  

^a UNIVERSITY OF MANITOBA   (Canada)

Author keywords

3H (+) ABA; ABA binding assays; Affinity; Association rate constant; Competition assays; Dissociation rate constant; Equilibrium dissociation constant; Radioligand binding assays; Saturation kinetics

Indexed keywords

EID: 60349118865     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-477-3_8     Document Type: Article

References (6)

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3. Razem, F.A., Luo, M., Liu, J.-H., Abrams, S.R., and Hill, R.D. (2004) Purification and characterization of a barley aleurone abscisic acid-binding protein. J. Biol. Chem. 279, 9922-9929.
4. Razem, F.A., El Kereamy, A., Abrams, S.R., and Hill, R.D. (2006) The RNA-binding protein FCA is an abscisic acid receptor. Nature 439, 290-294.
5. Liu, X., Yue, Y., Li, B., Nie, Y., Li, W., Wu, W.-H., and Ma, L. (2007) A G protein-coupled receptor is a plasma membrane receptor for the plant hormone abscisic acid. Science 315, 1712-1716.
6. Shen, Y.-Y., Wang, X.-F., Wu, F.-Q., Du, S.-Y., Cao, Z., Shang, Y., Wang, X.-L., Peng, C.-C., Yu, X.-C., Zhu, S.-Y., Fan, R.-C., Xu, Y.-H., and Zhang, D.-P. (2006) The Mg-chelatase H subunit is an abscisic acid receptor. Nature 443, 823-826.