Quantification of redox conditions in the nucleus

Methods in Molecular Biology

Volumn 464, Issue , 2008, Pages 303-317

  Go, Young Mi ^a   Pohl, Jan ^a   Jones, Dean P ^a  

^a EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Biotinylated iodoacetamide (BIAM); Glutathione; Isotope coded affinity tags (ICAT) analysis; Nuclear proteins; Nuclearredox state; Protein S glutathionylation; Redox Western blot; Thioredoxin reductase; Thioredoxin 1

Indexed keywords

EID: 60349113228     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60327-461-6_17     Document Type: Article

References (28)

1. Jones, D.P. (2006) Redefining oxidative stress. Antioxid. Redox Signal. 8, 1865-1879.
2. Hansen, J.M., Go, Y.M., and Jones, D.P. (2006) Nuclear and mitochondrial compartmentation of oxidative stress and redox signaling. Annu. Rev. Pharmacol. Toxicol. 46, 215-234.
3. Dijkwel, P.A. and Wenink, P.W. (1986) Structural integrity of the nuclear matrix: differential effects of thiol agents and metal chelators. J. Cell Sci. 84, 53-67.
4. Klug, A. and Rhodes, D. (1987) Zinc fingers: a novel protein fold for nucleic acid recognition. Cold Spring Harb. Symp. Quant. Biol. 52, 473-482.
5. De Capoa, A., Ferraro, M., Lavia, P., Pelliccia, F., and Finazzi-Agro, A. (1982) Silver staining of the nucleolus organizer regions (NOR) requires clusters of sulfhydryl groups. J Histochem. Cytochem. 30, 908-911.
6. Suthanthiran, M., Anderson, M.E., Sharma, V.K., and Meister, A. (1990) Glutathione regulates activation-dependent DNA synthesis in highly purified normal human T lymphocytes stimulated via the CD2 and CD3 antigens. Proc. Natl. Acad. Sci. USA 87, 3343-3347.
7. Sandstrom, B.E. and Marklund, S.L. (1990) Effects of variation in glutathione peroxidase activity on DNA damage and cell survival in human cells exposed to hydrogen peroxide and t-butyl hydroperoxide. Biochem. J. 271, 17-23.
8. Sen, C.K. and Packer, L. (1996) Antioxidant and redox regulation of gene transcription. FASEB J. 10, 709-720.
9. Hirota, K., Murata, M., Sachi, Y., Nakamura, H., Takeuchi, J., Mori, K., and Yodoi, J. (1999) Distinct roles of thioredoxin in the cytoplasm and in the nucleus. A two-step mechanism of redox regulation of transcription factor NF-kappaB. J. Biol. Chem. 274, 27891-27897.
10. Wei, S.J., Botero, A., Hirota, K., Bradbury, C.M., Markovina, S., Laszlo, A., Spitz, D.R., Goswami, P.C., Yodoi, J., and Gius, D. (2000) Thioredoxin nuclear translocation and interaction with redox factor-1 activates the activator protein-1 transcription factor in response to ionizing radiation. Cancer Res. 60, 6688-6695.
11. Bellomo, G., Vairetti, M., Stivala, L., Mirabelli, F., Richelmi, P., and Orrenius, S. (1992) Demonstration of nuclear compartmentalization of glutathione in hepatocytes. Proc. Natl. Acad. Sci. USA 89, 4412-4416.
12. Briviba, K., Fraser, G., Sies, H., and Ketterer, B. (1993) Distribution of the monochlorobimane-glutathione conjugate between nucleus and cytosol in isolated hepatocytes. Biochem. J. 294, 631-633.
13. Cotgreave, I.A. (2003) Analytical developments in the assay of intra- and extracellular GSH homeostasis: specific protein S-glutathionylation, cellular GSH and mixed disulphide compartmentalisation and interstitial GSH redox balance. Biofactors 17, 269-277.
14. Holmgren, A. and Fagerstedt, M. (1982) The in vivo distribution of oxidized and reduced thioredoxin in Escherichia coli. J. Biol. Chem. 257, 6926-6930.
15. Watson, W.H., Pohl, J., Montfort, W.R., Stuchlik, O., Reed, M.S., Powis, G., and Jones, D.P. (2003) Redox potential of human thioredoxin 1 and identification of a second dithiol/disulfide motif. J. Biol. Chem. 278, 33408-33415.
16. Janssen, Y.M. and Sen, C.K. (1999) Nuclear factor kappa B activity in response to oxidants and antioxidants. Methods Enzymol. 300, 363-374.
17. Padgett, C.M. and Whorton, A.R. (1995) S-nitrosoglutathione reversibly inhibits GAPDH by S-nitrosylation. Am. J. Physiol. 269, C739-749.
18. Stadtman, T.C. (2002) Discoveries of vitamin B12 and selenium enzymes. Annu. Rev. Biochem. 71, 1-16.
19. Gasdaska, P.Y., Gasdaska, J.R., Cochran, S., and Powis, G. (1995) Cloning and sequencing of a human thioredoxin reductase. FEBS Lett. 373, 5-9.
20. Mustacich, D. and Powis, G. (2000) Thioredoxin reductase. Biochem. J. 346, 1-8.
21. Soini, Y., Kahlos, K., Napankangas, U., Kaarteenaho-Wiik, R., Saily, M., Koistinen, P., Paaakko, P., Holmgren, A., and Kinnula, V.L. (2001) Widespread expression of thioredoxin and thioredoxin reductase in non-small cell lung carcinoma. Clin. Cancer Res. 7, 1750-1757.
22. Kim, J.R., Lee, S.M., Cho, S.H., Kim, J.H., Kim, B.H., Kwon, J., Choi, C.Y., Kim, Y.D., and Lee, S.R. (2004) Oxidation of thioredoxin reductase in HeLa cells stimulated with tumor necrosis factor-alpha. FEBS Lett. 567, 189-196.
23. Brigelius, R., Lenzen, R., and Sies, H. (1982) Increase in hepatic mixed disulphide and glutathione disulphide levels elicited by paraquat. Biochem. Pharmacol. 31, 1637-1641.
24. Brigelius, R., Muckel, C., Akerboom, T.P., and Sies, H. (1983) Identification and quantitation of glutathione in hepatic protein mixed disulfides and its relationship to glutathione disulfide. Biochem. Pharmacol. 32, 2529-2534.
25. Lash, L.H. and Jones, D.P. (1985) Distribution of oxidized and reduced forms of glutathione and cysteine in rat plasma. Arch. Biochem. Biophys. 240, 583-592.
26. Go, Y.M., Ziegler, T.R., Johnson, J.M., Gu, L., Hansen, J.M., and Jones, D.P. (2007) Selective protection of nuclear thioredoxin-1 and glutathione redox systems against oxidation during glucose and glutamine deficiency in human colonic epithelial cells. Free Radic. Biol. Med. 42, 363-370.
27. Sethuraman, M., McComb, M.E., Huang, H., Huang, S., Heibeck, T., Costello, C.E., and Cohen, R.A. (2004) Isotope-coded affinity tag (ICAT) approach to redox proteomics: identification and quantitation of oxidant-sensitive cysteine thiols in complex protein mixtures. J. Proteome Res. 3, 1228-1233.
28. Halvey, P.J., Watson, W.H., Hansen, J.M., Go, Y.M., Samali, A., and Jones, D.P. (2005) Compartmental oxidation of thiol-disulphide redox couples during epidermal growth factor signalling. Biochem. J. 386, 215-219.