Nuclear envelope formation in vitro: A sea urchin egg cell-free system

Methods in Molecular Biology

Volumn 464, Issue , 2008, Pages 207-223

  Byrne, Richard D ^a   Zhendre, Vanessa ^a   Larijani, Banafshé ^a   Poccia, Dominic L ^b  

^a IMPERIAL CANCER RESEARCH FUND   (United Kingdom)

^b AMHERST COLLEGE   (United States)

Author keywords

Cholesterol; Diacylglycerol; Liquid NMR; Liquid nuclear magnetic resonance; Nuclear envelope; Phosphatidylcholine; Phosphatidylinositol; Phospholipase C; PtdIns(4; 5)P2; Sea urchin

Indexed keywords

ECHINOIDEA;

EID: 60349111961     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60327-461-6_12     Document Type: Article

References (13)

1. Longo, F.J. and Anderson, E. (1968) The fine structure of pronuclear development and fusion in the sea urchin, Arbacia punctulata. J. Cell Biol. 39, 339-368.
2. Collas, P. and Poccia, D. (1995) Lipophilic organizing structures of sperm nuclei target membrane vesicle binding and are incorporated into the nuclear envelope. Dev. Biol. 169, 123-135.
3. Cothren, C.C. and Poccia, D.L. (1993) Two steps required for male pronucleus formation in the sea urchin egg. Exp. Cell Res. 205, 126-133.
4. Collas, P. and Poccia, D.L. (1995) Formation of the sea urchin male pronucleus in vitro: membrane-independent chromatin decondensation and nuclear envelope-dependent nuclear swelling. Mol. Reprod. Dev. 42, 106-113.
5. Larijani, B., Poccia, D.L., and Dickinson, L.C. (2000) Phospholipid identification and quantification of membrane vesicle subfractions by 31P-1H two-dimensional nuclear magnetic resonance. Lipids 35, 1289-1297.
6. Byrne, R., Garnier, M., Han, K., Dowicki, M., Totty, N., Cho, A., Pettit, R., Wakelam, M.J.O., Poccia, D., and Larijani, B. (2006) PLCg and phosphoinositides are highly enriched in membrane vesicles required for nuclear envelope assembly. Cell Signalling 19, 913-922.
7. Collas, P. and Poccia, D. (1996) Distinct egg membrane vesicles differing in binding and fusion properties contribute to sea urchin male pronuclear envelopes formed in vitro. J. Cell Sci. 109, 1275-1283.
8. Larijani, B. and Poccia, D. (2007) Protein and lipid signaling in membrane fusion: nuclear envelope assembly. Signal Transduction 7, 142-153.
9. Garnier-Lhomme, M., Dufourc, E.J., Larijani, B., and Poccia, D. (2007) Lipid Quantification and structure determination of nuclear envelope precursor membranes in the sea urchin. Methods Mol. Biol. in press.
10. Collas, P. and Poccia, D. (1998) Methods for studying in vitro assembly of male pronuclei using oocyte extracts from marine invertebrates: sea urchins and surf clams. Methods Cell Biol. 53, 417-452.
11. Byrne, R.D., Barona, T.M., Garnier, M., Koster, G., Katan, M., Poccia, D.L., and Larijani, B. (2005) Nuclear envelope assembly is promoted by phosphoinositide-specific phospholipase C with selective recruitment of phosphatidylinositol-enriched membranes. Biochem. J. 387, 393-400.
12. Meneses, P. and Glonek, T. (1988) High resolution 31P NMR of extracted phospholipids. J Lipid Res. 29, 679-689.
13. Foltz, K.R., Adams, N.L., and Runft, L.L. (2004) Echinoderm eggs and embryos: procurement and culture. Methods Cell Biol. 74, 39-74.