Electron spectroscopic imaging of the nuclear landscape

Methods in Molecular Biology

Volumn 464, Issue , 2008, Pages 415-423

  Ahmed, Kashif ^a   Li, Ren ^a   Bazett Jones, David P ^a  

^a HOSPITAL FOR SICK CHILDREN   (Canada)

Author keywords

Chromatin; Correlative microscopy; Electron spectroscopic imaging; Nuclear structure; Nucleus; Promyelocytic leukemia nuclear bodies

Indexed keywords

EID: 60349109666     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60327-461-6_23     Document Type: Article

References (18)

1. Cremer, T. and Cremer, C. (2001) Chromosome territories, nuclear architecture and gene regulation in mammalian cells. Nat. Rev. Genet. 2, 292-301.
2. Lamond, A. I. and Earnshaw, W. C. (1998) Structure and function in the nucleus. Science 280, 547-553.
3. Kruhlak, M. J., Lever, M. A., Fischle, W., Verdin, E., Bazett-Jones, D. P., and Hendzel, M. J. (2000) Reduced mobility of the alternate splicing factor (ASF) through the nucleoplasm and steady state speckle compartments. J. Cell. Biol. 150, 41-51.
4. Olins, A. L. and Olins, D. E. (1974) Spheroid chromatin units (nu bodies). Science 183, 330-332.
5. Miller, O. L., Jr. and Beatty, B. R. (1969) Visualization of nucleolar genes. Science 164, 955-957.
6. Bazett-Jones, D. P. and Hendzel, M. J. (1999) Electron spectroscopic imaging of chromatin. Methods 17, 188-200.
7. Dehghani, H., Dellaire, G., and Bazett-Jones, D. P. (2005) Organization of chromatin in the interphase mammalian cell. Micron 36, 95-108.
8. Bolzer, A., Kreth, G., Solovei, I., Koehler, D., Saracoglu, K., Fauth, C., Muller, S., Eils, R., Cremer, C., Speicher, M. R., and Cremer, T. (2005) Three-dimensional maps of all chromosomes in human male fibroblast nuclei and prometaphase rosettes. PLoS Biol. 3, e157.
9. van Driel, R., Fransz, P. F., and Verschure, P. J. (2003) The eukaryotic genome: a system regulated at different hierarchical levels. J. Cell Sci. 116, 4067-4075.
10. Dundr, M. and Misteli, T. (2001) Functional architecture in the cell nucleus. Biochem. J. 356, 297-310.
11. Boyle, S., Gilchrist, S., Bridger, J. M., Mahy, N. L., Ellis, J. A., and Bickmore, W. A. (2001) The spatial organization of human chromosomes within the nuclei of normal and emerin-mutant cells. Hum. Mol. Genet. 10, 211-219.
12. Misteli, T. (2004) Spatial positioning: A new dimension in genome function. Cell 119, 153-156.
13. Osborne, C. S., Chakalova, L., Brown, K. E., Carter, D., Horton, A., Debrand, E., Goyenechea, B., Mitchell, J. A., Lopes, S., Reik, W., and Fraser, P. (2004) Active genes dynamically colocalize to shared sites of ongoing transcription. Nat. Genet. 36, 1065-1071.
14. Chambeyron, S. and Bickmore, W. A. (2004) Chromatin decondensation and nuclear reorganization of the HoxB locus upon induction of transcription. Genes Dev. 18, 1119-1130.
15. Borden, K. L. (2002) Pondering the promyelocytic leukemia protein (PML) puzzle: possible functions for PML nuclear bodies. Mol. Cell. Biol. 22, 5259-5269.
16. Dellaire, G. and Bazett-Jones, D. P. (2004) PML nuclear bodies: Dynamic sensors of cellular stress and DNA damage. Bioessays 26, 963-977.
17. Eskiw, C. H., Dellaire, G., and Bazett-Jones, D. P. (2004) Chromatin contributes to structural integrity of promyelocytic leukemia bodies through a SUMO-1-independent mechanism. J. Biol. Chem. 279, 9577-9585.
18. Block, G. J., Eskiw, C. H., Dellaire, G., and Bazett-Jones, D. P. (2006) Transcriptional regulation is affected by subnuclear targeting of reporter plasmids to PML nuclear bodies. Mol. Cell. Biol. 26, 8814-8825.