Quantitative characterization of virus-like particles by asymmetrical flow field flow fractionation, electrospray differential mobility analysis, and transmission electron microscopy

Biotechnology and Bioengineering

Volumn 102, Issue 3, 2009, Pages 845-855

  Pease III, Leonard F ^a   Lipin, Daniel I ^b   Tsai, De Hao ^a,c   Zachariah, Michael R ^a,c   Lua, Linda H L ^b   Tarlov, Michael J ^a   Middelberg, Anton P J ^b  

^a NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY   (United States)

^b UNIVERSITY OF QUEENSLAND   (Australia)

^c UNIVERSITY OF MARYLAND   (United States)

Author keywords

Asymmetric flow field flow fractionation (affff); Electrospray differential mobility analysis (es dma); Influenza; Polyomavirus; Virus

Indexed keywords

ASYMMETRIC FLOW FIELD-FLOW FRACTIONATION (AFFFF); ELECTROSPRAY DIFFERENTIAL MOBILITY ANALYSIS (ES-DMA); INFLUENZA; POLYOMAVIRUS; VIRUS;

AMINES; COMPUTER VIRUSES; DRUG THERAPY; ELECTRON MICROSCOPES; ELECTRON MICROSCOPY; FLOW FIELDS; FLOW OF FLUIDS; FLOW PATTERNS; IMAGE SEGMENTATION; LIQUID CHROMATOGRAPHY; NUCLEIC ACIDS; ORGANIC ACIDS; POLYDISPERSITY; PRODUCT DEVELOPMENT; PROJECT MANAGEMENT; SIZE DISTRIBUTION; TRANSMISSION ELECTRON MICROSCOPY; VACCINES; VIRUSES;

FRACTIONATION;

GENOMIC DNA; MEMBRANE PROTEIN; VIRUS DNA;

ANALYTIC METHOD; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DNA PACKAGING; ELECTROSPRAY DIFFERENTIAL MOBILITY ANALYSIS; FIELD FLOW FRACTIONATION; GENE TARGETING; GENETIC ENGINEERING AND GENE TECHNOLOGY; INFLUENZA VIRUS; MULTI ANGLE LIGHT SCATTERING DETECTION; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYOMA VIRUS; QUANTITATIVE ASSAY; QUANTITATIVE TRAIT; TRANSMISSION ELECTRON MICROSCOPY; VIRUS LIKE AGENT;

ANIMALS; BIRDS; CAPSID; DNA; FRACTIONATION, FIELD FLOW; HEMAGGLUTININ GLYCOPROTEINS, INFLUENZA VIRUS; INFLUENZA A VIRUS; INSECTS; MICROSCOPY, ELECTRON, TRANSMISSION; PARTICLE SIZE; POLYOMAVIRUS; RECOMBINANT PROTEINS; VIRAL PROTEINS;

MURINE POLYOMAVIRUS; POLYOMAVIRUS;

EID: 60349103484     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22085     Document Type: Article

References (52)

1. Bacher G, Szymanski WW, Kaufman SL, Zollner P, Blaas D, Allmaier G. 2001. Charge-reduced nano electrospray ionization combined with differential mobility analysis of peptides, proteins, glycoproteins, non- covalent protein complexes and viruses. J Mass Spectrom 36(9):1038-1052.
2. Barouch DH, Harrison SC. 1994. Interactions among the major and minor coat proteins of polyomavirus. J Virol 68(6):3982-3989.
3. Boura E, Liebl D, Spisek R, Fric J, Marek M, Stokrova J, Holan V, Forstova J. 2005. Polyomavirus EGFP-pseudocapsids: Analysis of model particles for introduction of proteins and peptides into mammalian cells. FEBS Lett 579(29):6549-6558.
4. Charpilienne A, Nejmeddine M, Berois M, Parez N, Neumann E, Hewat E, Trugnan G, Cohen J. 2001. Individual rotavirus-like particles containing 120 molecules of fluorescent protein are visible in living cells. J Biol Chem 276(31):29361-29367.
5. Chuan YP, Fan YY, Lua L, Middelberg APJ. 2008. Quantitative analysis of virus-like particle size and distribution by field-flow fractionation. Biotechnol Bioeng 99(6):1425-1433.
6. DePalma A. 2004. PAT: Taking process monitoring to next level. Genet Eng News 24(15):46-7.
7. FDA. 2004. Guidance for industry: PAT-A framework for innovative pharmaceutical development, manufacturing, and quality assurance. http://www.fda.gov/cder/guidance/6419fnl.pdf.
8. Finch JT. 1974. Surface-structure of polyoma-virus. J Gen Virol 24:359-364.
9. Fligge C, Schafer F, Selinka HC, Sapp C, Sapp M. 2001. DNA-induced structural changes in the papillomavirus capsid. J Virol 75(16):7727-7731.
10. Forstova J, Krauzewicz N, Sandig V, Elliott J, Palkova Z, Strauss M, Griffin BE. 1995. Polyoma virus pseudocapsids as efficient carriers of hetero- logous DNA into mammalian cells. Hum Gene Ther 6(3):297-306.
11. Garcea RL, Gissmann L. 2004. Virus-like particles as vaccines and vessels for the delivery of small molecules. Curr Opin Biotechnol 15(6):513-517.
12. Gasteiger E, Hoogland C, Gattiker A, Duvaud S, Wilkins MR, Appel RD. 2005. In: JM W, editor. The proteomics protocols handbook. Totowa, NJ: Humana Press. p. 571-607.
13. Gedvilaite A, Frommel C, Sasnauskas K, Micheel B, Ozel M, Behrsing O, Staniulis J, Jandrig B, Scherneck S, Ulrich R. 2000. Formation of immunogenic virus-like particles by inserting epitopes into surface-exposed regions of hamster polyomavirus major capsid protein. Virology 273(1):21-35.
14. Gillock ET, Rottinghaus S, Chang D, Cai X, Smiley SA, An K, Consigli RA. 1997. Polyomavirus major capsid protein VP1 is capable of packaging cellular DNA when expressed in the baculovirus system. J Virol 71(4):2857-2865.
15. Gleiter S, Lilie H. 2003. Cell-type specific targeting and gene expression using a variant ofpolyoma VP1 virus-like particles. Biol Chem 384(2):247-255.
16. Gleiter S, Stubenrauch K, Lilie H. 1999. Changing the surface of a virus shell fusion of an enzyme to polyoma VP1. Protein Sci 8(12):2562-2569.
17. Hagensee ME, Olson NH, Baker TS, Galloway DA. 1994. 3-Dimensional structure of vaccinia virus-produced human papillomavirus type-1 capsids. J Virol 68(7):4503-4505.
18. Hinds WC. 1999. Aerosol technology: Properties, behavior, and measurement of airborne particles. New York: John Wiley & Sons. p. 84.
19. Hogan CJ, Kettleson EM, Lee MH, Ramaswami B, Angenent LT, Biswas P. 2005. Sampling methodologies and dosage assessment techniques for submicrometre and ultrafine virus aerosol particles. J Appl Microbiol 99(6):1422-1434.
20. Hogan CJ, Kettleson EM, Ramaswami B, Chen DR, Biswas P. 2006. Charge reduced electrospray size spectrometry of mega- and gigadalton complexes: Whole viruses and virus fragments. Anal Chem 78(3):844-852.
21. Huglin MB. 1972. Light scattering from polymer solutions. London, UK: Academic Press. p. 165-331.
22. Ionescu RM, Przysiecki CT, Liang XP, Garsky VM, Fan JA, Wang B, Troutman R, Rippeon Y, Flanagan E, Shiver J, Shi L. 2006. Pharmaceutical and immunological evaluation of human papillomavirus viruslike particle as an antigen carrier. J Pharm Sci 95(1):70-79.
23. Koutsky LA, Ault KA, Wheeler CM, Brown DR, Barr E, Alvarez FB, Chiacchierini LM, Jansen KU. 2002. A controlled trial of a human papillomavirus type 16 vaccine. N Engl J Med 347(21):1645-1651.
24. Kuzmanovic DA, Elashvili I, Wick C, O'Connell C, Krueger S. 2006. The MS2 coat protein shell is likely assembled under tension: A novel role for the MS2 bacteriophage A protein as revealed by small-angle neutron scattering. J Mol Biol 355(5):1095-1111.
25. Lute S, Aranha H, Tremblay D, Liang DH, Ackermann HW, Chu B, Moineau S, Brorson K. 2004. Characterization of coliphage PR772 and evaluation of its use for virus filter performance testing. Appl Environ Microbiol 70(8):4864-4871.
26. Lute S, Riordan W, Pease LF III, Tsai D-H, Levy R, Haque M, Martin J, Moroe I, Sato T, Morgan M, Krishnan M, Campbell J, Genest P, Dolan S, Tarrach K, Meyer A, the PDA Virus Filter Task Force, Zachariah MR, Tarlov MJ, Etzel M, Brorson KA. 2008. A consensus rating method for small virus-retentive filters. I. Method development. PDA J Pharm Sci Technol, in press.
27. Moreland RB, Montross L, Garcea RL. 1991. Characterization of the DNA- binding properties of the polyomavirus capsid protein-VP1. J Virol 65(3):1168-1176.
28. Noad R, Roy P. 2003. Virus-like particles as immunogens. Trends Microbiol 11(9):438 -444.
29. Pattenden LK, Middelberg APJ, Niebert M, Lipin DI. 2005. Towards the preparative and large-scale precision manufacture of virus-like particles. Trends Biotechnol 23(10):523-529.
30. Pawlita M, Muller M, Oppenlander M, Zentgraf H, Herrmann M. 1996. DNA encapsidation by viruslike particles assembled in insect cells from the major capsid protein VP1 of B-lymphotropic papovavirus. J Virol 70(11):7517-7526.
31. Pease LF, Tsai D-H, Zangmeister RA, Zachariah MR, Tarlov MJ. 2007. Quantifying the surface coverage of conjugate molecules on functio-nalized nanoparticles. J Phys Chem C 111(46):17155-17157.
32. Pease LF, Elliott JT, Tsai D-H, Zachariah MR, Tarlov MJ. 2008. Determination of protein aggregation with differential mobilityanalysis: Application to IgG antibody. Biotechnol Bioeng 101:1214-1222.
33. Pushko P, Tumpey TM, Bu F, Knell J, Robinson R, Smith G. 2005. Influenza virus-like particles comprised of the HA, NA, and M1 proteins ofH9N2 influenza virus induce protective immune responses in BALB/c mice. Vaccine 23(50):5751-5759.
34. Rayment I, Baker TS, Caspar DL, Murakami WT. 1982. Polyoma virus capsid structure at 22.5 A resolution. Nature 295(5845):110-115.
35. Salunke DM, Caspar DL, Garcea RL. 1986. Self-assembly of purified polyomavirus capsid protein VP1. Cell 46(6):895-904.
36. Satterly J. 1960. Formulae for volumnes, surface areas and radii of gyration of spheres, ellipsoids and spheroids. Math Gaz 44(347):15-19.
37. Schafer F, Florin L, Sapp M. 2002. DNA binding ofL1 is required for human papillomavirus morphogenesis in vivo. Virology 295(1):172-181.
38. Schmidt U, Gunther C, Rudolph R, Bohm G. 2001. Protein and peptide delivery via engineered polyomavirus-like particles. FASEB J 15(9):1646-1648.
39. Scolnick EM, McLean AA, West DJ, McAleer WJ, Miller WJ, Buynak EB. 1984. Clinical-evaluation in healthy-adults of a hepatitis-B vaccine made by recombinant DNA. J Am Med Assoc 251(21):2812-2815.
40. Shortt DW, Roessner D, Wyatt PJ. 1996. Absolute measurement of diameter distributions of particles using a multiangle light scattering photometer coupled with flow field-flow fractionation. Am Lab 17:21.
41. Siuzdak G, Bothner B, Yeager M, Brugidou C, Fauquet CM, Hoey K, Chang CM. 1996. Mass spectrometry and viral analysis. Chem Biol 3(1):45-48.
42. Thomas JJ, Bothner B, Traina J, Benner WH, Siuzdak G. 2004. Electrospray ion mobility spectrometry of intact viruses. Spectrosc Int J 18(1):31-36.
43. Tsai D-H, Zangmeister RA, Pease LF, Tarlov MJ, Zachariah MR. 2008. Gas-phase ion-mobility characterization of SAM functionalized Au nano-particles. J Phys Chem C 24(16):8483-8490.
44. Tsoka S, Holwill I, Hoare M. 1999. Virus-like particle analysis in yeast homogenate using a laser light-scattering assay. Biotechnol Bioeng 63(3):290-297.
45. Tsukamoto H, Kawano M-a, Inoue T, Enomoto T, Takahashi R-u, Yokoyama N, Yamamoto N, Imai T, Kataoka K, Yamaguchi Y, et al. 2007. Evidence that SV40 VP1-DNA interactions contribute to the assembly of 40-nm spherical viral particles. Genes Cells 12(11): 1267-1279.
46. Voronkova T, Kazaks A, Ose V, Ozel M, Scherneck S, Pumpens P, Ulrich R. 2007. Hamster polyomavirus-derived virus-like particles are able to transfer in vitro encapsidated plasmid DNA to mammalian cells. Virus Genes 34(3):303-314.
47. Wei Z, McEvoy M, Razinkov V, Polozova A, Li E, Casas-Finet J, Tous GI, Balu P, Pan AA, Mehta H, et al. 2007. Biophysical characterization of influenza virus subpopulations using field flow fractionation and multiangle light scattering: Correlation of particle counts, size distribution and infectivity. J Virol Methods 144(1-2):122-132.
48. Wiedensohler A. 1988. An approximation of the bipolar charge-distribution for particles in the sub-micron size range. J Aerosol Sci 19(3):387-389.
49. Wyatt PJ. 1993. Light scattering and the absolute characterization of macromolecules. Anal Chim Acta 272(1):1-40.
50. Yamada T, Iwasaki Y, Tada H, Iwabuki H, Chuah MKL, VandenDriessche T, Fukuda H, Kondo A, Ueda M, Seno M, et al. 2003. Nanoparticles for the delivery of genes and drugs to human hepatocytes. Nature Biotechnol 21(8):885-890.
51. Zimm BH. 1948. The scattering of light and the radial distribution function of high polymer solutions. J Chem Phys 16(12):1093-1099.
52. Zolotukhin S, Byrne BJ, Mason E, Zolotukhin I, Potter M, Chesnut K, Summerford C, Samulski RJ, Muzyczka N. 1999. Recombinant adeno-associated virus purification using novel methods improves infectious titer and yield. Gene Ther 6(6):973-985.