Rubidium efflux as a tool for the pharmacological characterisation of compounds with BK channel opening properties

Methods in Molecular Biology

Volumn 491, Issue , 2008, Pages 267-277

  McKay, Neil G ^a   Kirby, Robert W ^a   Lawson, Kim ^a  

^a SHEFFIELD HALLAM UNIVERSITY   (United Kingdom)

Author keywords

Atomic absorption spectrometry; BK channel; Channel openers; Drug discovery; Potassium channels; Rubidium efflux; Small molecules

Indexed keywords

EID: 60349096836     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-526-8_21     Document Type: Article

References (8)

1. Bennett P. B. and Guthrie H. R. (2003) Trends in ion channel drug discovery: advances in screening technologies. Trends in Biotechnology 21, 563-569.
2. Ebneth A. (2002) Ion channel screening technologies: will they revolutionise drug discovery? Drug Discovery Today 7, 227.
3. Birch P. J., Dekker L. V., James I. F., Southan A., and Cronk D. (2004) Strategies to identify ion channel modulators: current and novel approaches to target neuropathic pain. Drug Discovery Today 9, 410-418.
4. Zheng W., Spencer R. H., and Kiss L. (2004) High throughput assay technologies for ion channel drug discovery. Assay and Drug Development Technologies 2, 543-552.
5. Gill S., Gill R., Lee S. S., Hesketh J. C., Fedida D., Rezazadeh S., Stankovich L., and Liang D. (2003) Flux assays in high throughput screening of ion channels in drug discovery. Assay and Drug Development Technologies 1, 709-717.
6. Terstappen G. C. (1999) Functional analysis of native and recombinant ion channels using a high-capacity non-radioactive rubidium efflux assay. Analytical Biochemistry 272, 149-155.
7. Apostoli P. (2002) Elements in environmental and occupational medicine. Journal of Chromatography B 778, 63-97.
8. Hartness M. E., Brazier S. P., Peers C., Bateson A. N., Ashford M. L. J., and Kemp P. J. (2003) Post-transcriptional control of human maxiK potassium channel activity and acute oxygen sensitivity by chronic hypoxia. Journal of Biological Chemistry 278, 51422-51432.