Hormone-binding assay using living bacteria expressing eukaryotic receptors

Methods in Molecular Biology

Volumn 495, Issue , 2009, Pages 111-120

  Romanov, Georgy A ^a   Lomin, Sergey N ^a  

^a TIMIRYAZEV INSTITUTE OF PLANT PHYSIOLOGY   (Russian Federation)

Author keywords

Binding assay; Binding parameters; Hormone; Ligand; Receptor; Transgenic bacteria

Indexed keywords

BACTERIA (MICROORGANISMS); EUKARYOTA;

EID: 60349088816     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-477-3_10     Document Type: Article

References (11)

1. Gomperts, B.D., Kramer, I.M., and Tatham, P.E.R. (2003) Signal Transduction. Academic Press, San Diego et al.
2. Cuatrecasas, P., and Jacobs, S. (eds.) (1981) Membrane Receptors, Methods for Purification and Characterization. Chapman and Hall, London, N.Y.
3. Muramatsu, I., Tanaka, T., Suzuki, F., Li, Z., Hiraizumi-Hiraoka, Y., Anisuzzaman, A.S.M., Yamamoto, H., Horinouchi, T., and Morishima, S. (2005) Quantifying receptor properties: the tissue segment binding method - a powerful tool for the pharmacome analysis of native receptors. J. Pharmacol. 98, 331-339.
4. Suzuki, T., Miwa, K., Ishikawa, K., Yamada, H., Aiba, H., and Mizuno, T. (2001) The Arabidopsis sensor His-kinase, AHK4, can respond to cytokinins. Plant Cell Physiol. 42, 107-113.
5. Yamada, H., Suzuki, T., Terada, K., Takei, K., Ishikawa, K., Miwa, K., Yamashino, T., and Mizuno, T. (2001) The Arabidopsis AHK4 histidine kinase is a cytokinin-binding receptor that transduces cytokinin signals across the membrane. Plant Cell Physiol. 42, 1017-1023.
6. Spíchal, L., Rakova, N.Y., Riefler, M., Mizuno, T., Romanov, G.A., Strnad, M., and Schmülling, T. (2004) Two cytokinin receptors of Arabidopsis thaliana, CRE1/AHK4 and AHK3, differ in their ligand specificity in a bacterial assay. Plant Cell Physiol. 45, 1299-1305.
7. Romanov, G.A., Spíchal, L., Lomin, S.N., Strnad, M., and Schmülling, T. (2005) A live cell hormone-binding assay on transgenic bacteria expressing a eukaryotic receptor protein. Analyt. Biochem. 347, 129-134.
8. Romanov, G.A., Lomin, S.N., and Schmülling, T. (2006) Biochemical characteristics and ligand-binding properties of Arabidopsis cytokinin receptor AHK3 compared to CRE1/AHK4 as revealed by a direct binding assay. J. Exp. Botany, 57, 4051-4058.
9. Heyl, A., Wulfetange, K., Pils, B., Nielsen, N., Romanov, G.A., and Schmülling, T. (2007) Evolutionary proteomics identifies amino acids essential for ligand-binding of the cytokinin receptor CHASE domain. BMC Evolutionary Biol. 7, 62
10. Sambrook, J., and Russell, D.W. (2001) Molecular Cloning: a Laboratory Manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
11. Lomin S.N., and Romanov, G.A. (2008) The analysis of hormone-receptor interaction. Theoretical and practical aspects. Russian J. Plant Physiol. 55, 259-273