PilR, a transcriptional regulator for pilin and other genes required for Fe(III) reduction in Geobacter sulfurreducens

Journal of Molecular Microbiology and Biotechnology

Volumn 16, Issue 3-4, 2009, Pages 146-158

  Juárez, Katy ^a,d   Kim, Byoung Chan ^a   Nevin, Kelly ^a   Olvera, Leticia ^d   Reguera, Gemma ^a,b   Lovley, Derek R ^a   Methé, Barbara A ^c  

^a UNIVERSITY OF MASSACHUSETTS   (United States)

^b Michigan State University ^*  (United States)

^c J CRAIG VENTER INSTITUTE   (United States)

^d INSTITUTO DE BIOTECNOLOGÍA   (Mexico)

Author keywords

Electron transfer; Fe(III) reduction; Geobacter sulfurreducens; PilR; Type IV pili

Indexed keywords

BACTERIAL PROTEINS; FERRIC COMPOUNDS; FIMBRIAE PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; GENES, REGULATOR; GEOBACTER; OXIDATION-REDUCTION; TRANSCRIPTION, GENETIC;

GEOBACTER SULFURREDUCENS; PILA;

EID: 60149083685     PISSN: 14641801     EISSN: None     Source Type: Journal    
DOI: 10.1159/000115849     Document Type: Article

References (44)

1. 54-dependent promoter sequences. Nucleic Acids Res 1999;27:4305-4313.
2. Bond DR, Lovley DR: Electricity production by Geobacter sulfurreducens attached to electrodes. Appl Environ Microbiol 2003;69:1548-1555.
3. Brazma A, Hingamp P, Quackenbush J, Sherlock G, Spellman P, Stoeckert C, Aach J, Ansorge W, Ball CA, Causton HC, Gaasterland T, Glenisson P, Holstege FC, Kim IF, Markowitz V, Matese JC, Parkinson H, Robinson A, Sarkans U, Schulze-Kremer S, Stewart J, Taylor R, Vilo J, Vingron M: Minimum information about a microarray experiment (MIAME) - towards standards for microarray data. Nat Genet 2001;29:365-371.
4. Butler JE, Kaufmann F, Coppi MV, Núñez C, Lovley DR: MacA, a diheme c-type cytochrome involved in Fe(III) reduction by Geobacter sulfurreducens. J Bacteriol 2004;186:4042-4045.
5. Caccavo F, Lonergan DJ, Lovley DR, Davis M, Stolz JF, McInerney MJ: Geobacter sulfurreducens sp. nov., a hydrogen- and acetate-oxidizing dissimilatory metal-reducing microorganism. Appl Environ Microbiol 1994:60:3752-3759.
6. Coppi MV, Leang C, Sandler SJ, Lovley DR: Development of a genetic system for Geobacter sulfurreducens. Appl Environ Microbiol 2001;67:3180-3187.
7. Cotter PA, Melville SB, Albrecht JA, Gunsalus RP: Aerobic regulation of cytochrome d oxidase (cydAB) operon expression in Escherichia coli: roles of Fnr and ArcA in repression and activation. Mol Microbiol 1997;25:605-615.
8. Esteve-Núñez A, Rothermich M, Sharma M, Lovley DR: Growth of Geobacter sulfurreducens under nutrient-limiting conditions in continuous culture. Environ Microbiol 2005;7:641-648.
9. Govantes F, Albrecht JA, Gunsalus RP: Oxygen regulation of the Escherichia coli cytochrome d oxidase (cydAB) operon: roles of multiple promoters and the Fnr-1 and Fnr-2 binding sites. Mol Microbiol 2000;37:1456-1469.
10. Hobbs M, Collie ES, Free PD, Livingston SP, Mattick JS: PilS and PilR, a two-component transcriptional regulatory system controlling expression of type IV fimbriae in Pseudomonas aeruginosa. Mol Microbiol 1993;7:669-682.
11. Holmes DE, Bond DR, O'Neil RA, Reimers CE, Lovley DR: Microbial communities associated with electrodes harvesting electricity from a variety of aquatic sediments. Microb Ecol 2004;48:178-190.
12. Holmes DE, Swades KC, Nevin K, Mehta T, Methé B, Liu A, Ward J, Woodard T, Webster JR, Lovley DR: Microarray and genetic analysis of electron transfer to electrodes in Geobacter sulfurreducens. Environ Microbiol 2006;8:1805-1815.
13. Jin S, Ishimoto KS, Lory S: PilR a transcriptional regulator of piliation in Pseudomonas aeruginosa, binds to a cis-acting sequence upstream if the pilin gene promoter. Mol Microbiol 1994;14:1049-1057.
14. Karlin S, Brocchieri L, Mrazek J, Kaiser D: Distinguishing features of δ-proteobacterial genomes. Proc Natl Acad Sci USA 2006;103:11352-11357.
15. Kim BC, Leang C, Ding YH, Glaven RH, Coppi MV, Lovley DR: OmcF, a putative c-type monoheme outer membrane cytochrome required for the expression of other outer membrane cytochromes in Geobacter sulfurreducens. J Bacteriol 2005;187:4505-4513.
16. Kim BC, Qian X, Leang C, Coppi MV, Lovley DR: Two putative c-type multiheme cytochromes required for the expression of omcB, an outer membrane protein essential for optimal Fe(III) reduction in Geobacter sulfurreducens. J Bacteriol 2006;188:3138-3142.
17. Kovach ME, Phillips RW, Elzer PH, Roop RM 2nd, Peterson KM: pBBR1MCS: a broad-host-range cloning vector. Biotechniques 1994;16:800-802.
18. Leang C, Coppi MV, Lovley DR: OmcB, a c-type polyheme cytochrome, involved in Fe(III) reduction in Geobacter sulfurreducens. J Bacteriol 2003;185:2096-2103.
19. Leang C, Lovley DR: Regulation of two highly similar genes, omcB and omcC, in a 10 kb chromosomal duplication in Geobacter sulfurreducens. Microbiology 2005a;151:1761-1767.
20. Leang C, Adams LA, Chin KJ, Nevin KP, Methé BA, Webster J, Sharma ML, Lovley DR: Adaptation to disruption of the electron transfer pathway for Fe(III) reduction in Geobacter sulfurreducens. J Bacteriol 2005b;187:5918-5926.
21. -ΔΔCT method. Methods 2001;25:402-408.
22. Lloyd JR, Leang C, Hodges Myerson AL, Coppi MV, Cuifo S, Methé B, Sandler SJ, Lovley DR: Biochemical and genetic characterization of PpcA, a periplasmic c-type cytochrome in Geobacter sulfurreducens. Biochem J 2003;369:153-161.
23. Lovley DR, Phillips EJ: Novel mode of microbial energy metabolism organic carbon oxidation coupled to dissimilatory reduction of iron or manganese. Appl Environ Microb 1988;54:1472-1480.
24. Lovley DR: Fe(III) and Mn(IV) Reduction;in Lovley DR (ed): Environmental Microbe-Metal Interactions. ASM Press, Washington, 2000, pp 3-30.
25. Lovley DR, Holmes DE, Nevin KP: Dissimilatory Fe(III) and Mn(IV) reduction. Adv Microb Physiol 2004;49:219-286.
26. Lovley DR: Bug juice: harvesting electricity with microorganisms. Nat Rev Microbiol 2006;4:497-508.
27. Machado P, Felix R, Rodrigues R, Oliveira S, Rodrigues-Pousada C: Characterization and expression analysis of the cytochrome bd oxidase operon from Desulfovibrio gigas. Curr Microbiol 2006;52:274-281.
28. Mattick JS: Type IV pili and twitching motility. Annu Rev Microbiol 2002;56:289-314.
29. Mehta T, Coppi MV, Childers SE, Lovley DR: Outer membrane c-type cytochromes required for Fe(III) and Mn(IV) oxide reduction in Geobacter sulfurreducens. Appl Environ Microbiol 2005;71:8634-8641.
30. Mehta T, Childers SE, Glaven RH, Lovley DR, Mester T: A putative multicopper protein secreted by an atypical type II secretion system involved in the reduction of insoluble electron acceptors in Geobacter sulfurreducens. Microbiology 2006;152:2257-2264.
31. Methé BA, Nelson KE, Eisen JA, Paulsen IT, Nelson W, Heidelberg JF, Wu D, Wu M, Ward N, Beanan MJ, Dodson RJ, Madupu R, Brinkac LM, Daugherty SC, DeBoy RT, Durkin AS, Gwinn M, Kolonay JF, Sullivan SA, Haft DH, Selengut J, Davidsen TM, Zafar N, White O, Tran B, Romero C, Forberger HA, Weidman J, Khouri H, Feldblyum TV, Utterback TR, Van Aken SE, Lovley DR, Fraser CM: Genome of Geobacter sulfurreducens: metal reduction in subsurface environments. Science 2003;302:1967-1969.
32. Methé BA, Webster J, Nevin KP, Butler J, Lovley DR: DNA microarray analysis of nitrogen fixation and Fe(III) reduction in Geobacter sulfurreducens. Appl Environ Microbiol 2005;71:2530-2538.
33. 54 bacterial enhancer-binding protein family: mechanism of action and phylogenetic relationship of their functional domains. J Bacteriol 1993;175:6067-6074.
34. Murphy KC, Campellone KC, Poteete AR: PCR-mediated gene replacement in Escherichia coli. Gene 2000;246:321-330.
35. Raleigh EA, Lech K, Brent, R: 1989; in Ausubel FM, et al (eds): Current Protocols in Molecular Biology. New York, Publishing Associates and Wiley Interscience, 1988, unit 1.4.
36. Reguera G, McCarthy KD, Mehta T, Nicoll JS, Tuominen MT, Lovley DR: Extracellular electron transfer via microbial nanowires. Nature 2005;435:1098-1101.
37. Reguera G, Pollina R, Nicoll JS, Lovley DR: Possible nonconductive role of Geobacter sulfurreducens pilus nanowires in biofilm formation. J Bacteriol 2007;189:2125-2127.
38. Sambrook J, Fritsch JE, Maniatis T: Molecular Cloning: A Laboratory Manual, ed 2. Cold Spring Harbor, Cold Spring Harbor Laboratory Press, 1989.
39. Smith PK, Krohn RI, Hermanson GT, Mallia KA, Garter MD, Provenzano EK, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC: Measurement of protein using biocinchoninic acid. Anal Biochem 1985;150:76-85.
40. 54- dependent transcriptional activators. J Bacteriol 2003;185:1757-1767.
41. 54- dependent enhancer binding proteins in Pirellula species strain 1. FEMS Microbiol Lett 2004;230:215-225.
42. Tusher VG, Tibshirani R, Chu G: Significance analysis of microarrays applied to the ionizing radiation response. Proc Natl Acad Sci USA 2001;98:5116-5121.
43. Wu S, Kaiser D: Regulation of Expression of the pilA Gene in Myxococcus xanthus. J Bacteriol 1997;179:7748-7758.
44. Yan B, Methé BA, Lovley DR, Krushkal J: Computational prediction of conserved operons and phylogenetic footprinting of transcription regulatory elements in the metal-reducing bacterial family Geobacteraceae. J Theor Biol 2004;230:133-144.