Improving detection in capillary electrophoresis with laser induced fluorescence via a bubble cell capillary and laser power adjustment

Biomedical Chromatography

Volumn 23, Issue 1, 2009, Pages 42-47

  Rodat, Audrey ^a,b   Gavard, Pierre ^a   Couderc, François ^a  

^a Université Paul Sabatier   (France)

^b Picometrics   (France)

Author keywords

Antibody; Beta blocker; Bubble cell; Capillary electrophoresis; Laser induced fluorescence; Protein

Indexed keywords

CELLS; CHEMICAL DETECTION; CYTOLOGY; FLUORESCENCE; FUSED SILICA; LASER OPTICS; LASER PRODUCED PLASMAS;

BETA-BLOCKERS; BUBBLE CELLS; FUSED-SILICA CAPILLARIES; LASER INDUCED FLUORESCENCE; LASER INDUCED FLUORESCENCE DETECTION; LASER POWER; PATHLENGTHS; PHOTO DEGRADATION; POWER ADJUSTMENTS; UV DETECTION;

CAPILLARY ELECTROPHORESIS;

AROMATIC COMPOUND; RIBOFLAVIN; SILICON DIOXIDE;

ARTICLE; BUBBLE CELL CAPILLARY; CAPILLARY; CAPILLARY CHROMATOGRAPHY; CAPILLARY ELECTROPHORESIS; CONTROLLED STUDY; FLUORESCENCE ANALYSIS; ILLUMINATION; INTERMETHOD COMPARISON; LASER; LASER INDUCED FLUORESCENCE DETECTION; LIGHT INTENSITY; PHOTODEGRADATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; SENSITIVITY ANALYSIS; SILVER STAINING;

EID: 59949100673     PISSN: 02693879     EISSN: 10990801     Source Type: Journal    
DOI: 10.1002/bmc.1080     Document Type: Article

References (24)

1. Bayle C, Issac C, Salvayre R, Couderc F and Caussé E. Assay of total homocysteine and other thiols by capillary electrophoresis and laser-induced fluorescence detection. Pre-analytical and analytical conditions. Journal of Chromatography A 2002; 979: 255-260.
2. Bayle C, Siri N, Poinsot V, Treilhou M, Caussé E and Couderc F. Analysis of tryptophan and tyrosine in cerebrospinal fluid by capillary electrophoresis and 'ball lens' UV-pulsed laser-induced fluorescence detection. Journal of Chromatography A 2003; 1013: 123-130.
3. Bayle C, Poinsot V, Fournier-Noel C and Couderc F, Laser-induced fluorescence detection. In Electrokinetic Chromatography: Theory, Instrumentation and Applications, Pyell U (ed.). Wiley: Chichester, 2006; 263-280.
4. Cole RO, Hiller DI, Chwojdak CA and Sepaniak MJ. Evaluation of extended light path capillaries for use in capillary electrophoresis with laser-induced fluorescence detection. Journal of Chromatogr A 1996; 736: 239-245.
5. Frost M, Köhler H and Blaschke G. Determination of LSD in blood by capillary electrophoresis with laser-induced fluorescence detection. Journal of Chromatography B: Biomedical Science Applications 1997; 693: 313-319.
6. Hapuarachchi S, Janaway GA and Aspinwall CA. Capillary electrophoresis with a UV light-emitting diode source for chemical monitoring of native and derivatized fluorescent compounds. Electrophoresis 2006; 27: 4052-4059.
7. Hunt G and Nashabeh W. Capillary electrophoresis sodium dodecyl sulfate nongel sieving analysis of a therapeutic recombinant monoclonal antibody: a biotechnology perspective. Analytical Chemistry 1999; 71: 2390-2397.
8. Ibrahim H, Bouajila J, Siri N, Rozing G, Nepveu F and Couderc F. Very small injected samples to study chloroquine and quinine in human serum using capillary-LC and native fluorescence. Journal of Chromatography B: Analytical Technology in Biomedicine and Life Sciences 2007; 850: 481-487.
9. Johnson ME and Landers JP. Fundamentals and practice for ultrasensitive laser-induced fluorescence detection in microanalytical systems. Electrophoresis 2004; 25: 3513-3527.
10. Krivácsy Z, Gelencsér A, Hlavay J, Kiss G and Sárvári Z. Electrokinetic injection in capillary electrophoresis and its application to the analysis of inorganic compounds. Journal of Chromatography A 1999; 834: 21-44.
11. Lacroix M, Poinsot V, Fournier C and Couderc F. Laser-induced fluorescence detection schemes for the analysis of proteins and peptides using capillary electrophoresis. Electrophoresis 2005; 26: 2608-2621.
12. Law WS, Zhao JH and Li SFY. On-line sample enrichment for the determination of proteins by capillary zone electrophoresis with poly(vinyl alcohol)-coated bubble cell capillaries. Electrophoresis 2005; 26: 3486-3494.
13. Malá Z, Krivánková L, Gebauer P and Bocek P. Contemporary sample stacking in CE: a sophisticated tool based on simple principles. Electrophoresis 2007; 28: 243-253.
14. Masukawa Y. Separation and determination of basic dyes formulated in hair care products by capillary electrophoresis. Journal of Chromatography A 2006; 1108: 140-144.
15. Mathies R, Peck K and Stryer L. Optimization of high-sensitivity fluorescence detection. Analytical Chemistry 1990; 62: 1786-1791.
16. Michels DA, Brady LJ, Guo A and Balland A Fluorescent derivatization method of proteins for characterization by capillary electrophoresis-sodium dodecyl sulfate with laser-induced fluorescence detection. Analytical Chemistry 2007; 79: 5963-5971.
17. Mrestani Y and Neubert R, Separation of etoposide phosphate and methotrexate by capillary zone electrophoresis using UV detection with a high sensitivity cell. Electrophoresis 1998; 19: 3022-3025.
18. Pyell U. Micellar electrokinetic chromatography - from theoretical concepts to real samples. Fresenius Journal of Analytical Chemistry 2001; 371: 691-703.
19. Rodat A, Kalck F, Feurer B, Poinsot V and Couderc F. An ellipsoidal mirror for detection of laser-induced fluorescence in capillary electrophoresis system: applications for labelled antibody analysis. Electrophoresis 2008; 29: 740-746.
20. Salas-Solano O, Tomlinson B, Du S, Parker M, Strahan A and Ma S. Optimization and validation of a quantitative capillary electrophoresis sodium dodecyl sulfate method for quality control and stability monitoring of monoclonal antibodies. Analytical Chemistry 2006; 78: 6583-6594.
21. Siméon N, Chatelut E, Canal P, Nertz M and Couderc F. Anthracycline analysis by capillary electrophoresis. Application to the analysis of daunorubicine in Kaposi sarcoma tumor. Journal of Chromatography A 1999; 853: 449-454.
22. Siri N, Riollet P, Bayle C and Couderc F. Automated large-volume sample stacking procedure to detect labelled peptides at picomolar concentration using capillary electrophoresis and laser-induced fluorescence detection. Journal of Chromatography B: Analytical Technology in Biomedical Life Science 2003; 793: 151-157.
23. Van den Beld CMB. Laser induced fluorescence detection in HPLC and its application in bioanalysis. PhD thesis, Leiden, University, 1991.
24. Xue Y and Yeung ES. Characterization of band broadening in capillary electrophoresis due to nonuniform capillary geometries. Analytical Chemistry 1994; 66: 3575-3580.