Transfection-induced defects in dynein-driven transport: Evidence that ICs mediate cargo-binding

Cell Motility and the Cytoskeleton

Volumn 66, Issue 2, 2009, Pages 80-89

  Towns, William L ^a   Tauhata, Sinji B F ^a   Vaughan, Patricia S ^a   Vaughan, Kevin T ^a  

^a UNIVERSITY OF NOTRE DAME   (United States)

Author keywords

Cytoplasmic dynein; Dynactin; Organelle transport; Phosphorylation

Indexed keywords

DYNACTIN; DYNEIN ADENOSINE TRIPHOSPHATASE; HOLOENZYME; PROTEIN SUBUNIT;

ANALYSIS; ANIMAL CELL; ARTICLE; CELL ORGANELLE; COMPETITION; COMPLEX FORMATION; DEPHOSPHORYLATION; DIMERIZATION; ENDOSOME; GEL; GENETIC TRANSFECTION; GOLGI COMPLEX; LIGHT CHAIN; LYSOSOME; NONHUMAN; PHOSPHORYLATION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; WILD TYPE;

ANIMALS; BIOLOGICAL TRANSPORT; CERCOPITHECUS AETHIOPS; COS CELLS; CYTOSKELETON; DYNEIN ATPASE; GOLGI APPARATUS; GREEN FLUORESCENT PROTEINS; MICROTUBULE-ASSOCIATED PROTEINS; ORGANELLES; PHOSPHORYLATION; PROTEIN SUBUNITS; TRANSFECTION;

EID: 59949098726     PISSN: 08861544     EISSN: 10970169     Source Type: Journal    
DOI: 10.1002/cm.20327     Document Type: Article

References (30)

1. Aniento F, Emans N, Griffiths G, Gruenberg J. 1993. Cytoplasmic dynein-dependent vesicular transport from early to late endo-somes. J Cell Biol 123(6 Part 1):1373-1387.
2. Bowman AB, Patel-King RS, Benashski SE, McCaffery JM, Goldstein LS, King SM. 1999. Drosophila roadblock and Chlamydomonas LC7: A conserved family of dynein-associated proteins involved in axonal transport, flagellar motility, and mitosis. J Cell Biol 146(1):165-180.
3. Burkhardt JK, Echeverri CJ, Nilsson T, Vallee RB. 1997. Overexpres-sion of the dynamitin (p50) subunit of the dynactin complex disrupts dynein-dependent maintenance of membrane organelle distribution. J Cell Biol 139(2):469-484.
4. Deacon SW, Serpinskaya AS, Vaughan PS, Lopez Fanarraga M, Ver-nos I, Vaughan KT, Gelfand VI. 2003. Dynactin is required for bidirectional organelle transport. J Cell Biol 160(3):297-301.
5. Echeverri CJ, Paschal BM, Vaughan KT, Vallee RB. 1996. Molecular characterization of the 50-kD subunit of dynactin reveals function for the complex in chromosome alignment and spindle organization during mitosis. J Cell Biol 132(4):617-633.
6. Gill SR, Schroer TA, Szilak I, Steuer ER, Sheetz MP, Cleveland DW. 1991. Dynactin, a conserved, ubiquitously expressed compo-nent of an activator of vesicle motility mediated by cytoplas-mic dynein. J Cell Biol 115(6):1639-1650.
7. Holleran EA, Tokito MK, Karki S, Holzbaur EL. 1996. Centractin (ARP1) associates with spectrin revealing a potential mechanism to link dynactin to intracellular organelles. J. Cell. Biol. 135:1815-1829.
8. Holzbaur EL, Hammarback JA, Paschal BM, Kravit NG, Pfister KK, Vallee RB. 1991. Homology of a 150K cytoplasmic dynein-associated polypeptide with the Drosophila gene Glued. Nature 351(6327):579-583.
9. Karki S, Holzbaur EL. 1995. Affinity chromatography demonstrates a direct binding between cytoplasmic dynein and the dynactin complex. J Biol Chem 270(48):28806-28811.
10. King SJ, Brown CL, Maier KC, Quintyne NJ, Schroer TA. 2003. Analysis of the dynein-dynactin Interaction in vitro and in vivo. Mol Biol Cell 14(12):5089-5097.
11. King SM, Barbarese E, Dillman JF, III, Patel-King RS, Carson JH, Pfister KK. 1996. Brain cytoplasmic and flagellar outer arm dyneins share a highly conserved Mr 8,000 light chain. J. Biol. Chem. 271(32):19358-19366.
12. King SM, Barbarese E, Dillman JF, 3rd, Benashski SE, Do KT, Patel-King RS, Pfister KK. 1998. Cytoplasmic dynein contains a family of differentially expressed light chains. Biochemistry 37:15033-15041.
13. Lo KW, Naisbitt S, Fan JS, Sheng M, Zhang M. 2001. The 8-kDa dynein light chain binds to its targets via a conserved (K/R)XTQT motif. J Biol Chem. 276:14059-14066.
14. Mok YK, Lo KW, Zhang M. 2001. Structure of Tctex-1 and its interaction with cytoplasmic dynein intermediate chain. J Biol Chem. 276:14067-14074.
15. Niclas J, Allan VJ, Vale RD. 1996. Cell cycle regulation of dynein association with membranes modulates microtubule-based or-ganelle transport. J Cell Biol 133(3):585-593.
16. Paschal BM, Mikami A, Pfister KK, Vallee RB. 1992. Homology of the 74-kD cytoplasmic dynein subunit with a flagellar dynein polypeptide suggests an intracellular targeting function. J Cell Biol 118(5):1133-1143.
17. Paschal BM, Shpetner HS, Vallee RB. 1987. MAP 1C is a microtu-bule-activated ATPase which translocates microtubules in vitro and has dynein-like properties. J Cell Biol 105(3):1273-1282.
18. Pfister KK, Fisher EM, Gibbons IR, Hays TS, Holzbaur EL, McIntosh JR, Porter ME, Schroer TA, Vaughan KT, Witman GB and others. 2005. Cytoplasmic dynein nomenclature. J Cell Biol 171(3):411-413.
19. Purohit A, Tynan SH, Vallee R, Doxsey SJ. 1999. Direct interaction of pericentrin with cytoplasmic dynein light intermediate chain contributes to mitotic spindle organization. J Cell Biol 147(3): 481-492.
20. Quintyne NJ, Gill SR, Eckley DM, Crego CL, Compton DA, Schroer TA. 1999. Dynactin is required for microtubule anchoring at centrosomes. J Cell Biol 147(2):321-334.
21. Schroer TA, Sheetz MP. 1991. Two activators of microtubule-based vesicle transport. J Cell Biol 115(5):1309-1318.
22. Susalka SJ, Nikulina K, Salata MW, Vaughan PS, King SM, Vaughan KT, Pfister KK. 2002. The roadblock light chain binds a novel region of the cytoplasmic dynein intermediate chain. J Biol Chem 20:20.
23. Tai AW, Chuang JZ, Bode C, Wolfrum U, Sung CH. 1999. Rhodop-sin's carboxy-terminal cytoplasmic tail acts as a membrane receptor for cytoplasmic dynein by binding to the dynein light chain Tctex-1. Cell 97:877-887.
24. Vale RD. 2003. The molecular motor toolbox for intracellular transport. Cell 112(4):467-480.
25. Vallee RB, Williams JC, Varma D, Barnhart LE. 2004. Dynein: An ancient motor protein involved in multiple modes of transport. J Neurobiol 58(2):189-200.
26. Vaughan KT, Vallee RB. 1995. Cytoplasmic dynein binds dynactin through a direct interaction between the intermediate chains and p150 Glued. J. Cell Biol. 131:1507-1516.
27. Vaughan PS, Leszyk JD, Vaughan KT. 2001. Cytoplasmic dynein intermediate chain phosphorylation regulates binding to dynac-tin. J Biol Chem 276(28):26171-26179.
28. Waterman-Storer CM, Holzbaur EL. 1996. The product of the Dro-sophila gene, Glued, is the functional homologue of the p150Glued component of the vertebrate dynactin complex. J Biol Chem 271(2):1153-1159.
29. Wilkerson CG, King SM, Koutoulis A, Pazour GJ, Witman GB. 1995. The 78,000 Mr intermediate chain of Chlamydomonas outer arm dynein is a WD-repeat protein required for arm assembly. J. Cell Biol. 129:169-178.
30. Yano H, Lee FS, Kong H, Chuang JZ, Arevalo JC, Perez P, Sung CH, Chao MV. 2001. Association of Trk neurotrophin receptors with components of the cytoplasmic dynein motor. J Neurosci 21(3):RC125.