메뉴 건너뛰기




Volumn 8, Issue 2, 2009, Pages 343-347

A practical HPLC method to measure reduced (GSH) and oxidized (GSSG) glutathione concentrations in animal tissues

Author keywords

GSH; GSSG; HPLC method; Liver; Wistar rat

Indexed keywords


EID: 59849122400     PISSN: 16805593     EISSN: 1993601X     Source Type: Journal    
DOI: None     Document Type: Article
Times cited : (41)

References (21)
  • 1
    • 0037093920 scopus 로고    scopus 로고
    • High performance liquid chromatographic assay for the quantitation of total glutathione in plasma
    • PMID: 12008143, DOI: 10.1016/S0731-7085(01)00658-6
    • Abukhalaf, I.K., N.A. Silvestrov, J.M. Menter, D.A. von Deutsch, M.A. Bayorh, R.R. Socci Agaba and A. Ganafa, 2002. High performance liquid chromatographic assay for the quantitation of total glutathione in plasma. J. Pharmaceut. Biomed. Anal., 28: 637-643. DOI: 10.1016/S0731-7085(01)00658-6. PMID: 12008143.
    • (2002) J. Pharmaceut. Biomed. Anal , vol.28 , pp. 637-643
    • Abukhalaf, I.K.1    Silvestrov, N.A.2    Menter, J.M.3    von Deutsch, D.A.4    Bayorh, M.A.5    Socci Agaba, R.R.6    Ganafa, A.7
  • 2
    • 0023257674 scopus 로고
    • Formation and reduction of glutathione protein mixed disulfides during oxidative stress. A study with isolated hepatocytes and menadione (2-methyl-1, 4-naphthoquinone)
    • PMID: 3593416, DOI: 10.1016/0006-2952(87)90087-6
    • Bellomo, G., F. Mirabelli, D. Di Monte, P. Richelmi, H. Thor, C. Orrenius and S. Orrenius, 1987. Formation and reduction of glutathione protein mixed disulfides during oxidative stress. A study with isolated hepatocytes and menadione (2-methyl-1, 4-naphthoquinone). Biochem. Pharmacol., 36 (8): 1313-1320. DOI: 10.1016/0006-2952(87)90087-6. PMID: 3593416.
    • (1987) Biochem. Pharmacol , vol.36 , Issue.8 , pp. 1313-1320
    • Bellomo, G.1    Mirabelli, F.2    Di Monte, D.3    Richelmi, P.4    Thor, H.5    Orrenius, C.6    Orrenius, S.7
  • 3
    • 0035809788 scopus 로고    scopus 로고
    • Cereser, C., J. Guichard, J. Drai, E. Bannier, I. Garcia. S. Boget, P. Parvaz and A. Revol, 2001. Quantization of reduced and total glutathione at the femtomole level by high-performance liquid chromatography with fluorescence detection: Application to red blood cells and cultured fibroblasts. J. Chromatograp. B: Biomed. Sci. Applied, 752: 123-132. DOI: 10.1016/S0378-4347(00) 00534-X.
    • Cereser, C., J. Guichard, J. Drai, E. Bannier, I. Garcia. S. Boget, P. Parvaz and A. Revol, 2001. Quantization of reduced and total glutathione at the femtomole level by high-performance liquid chromatography with fluorescence detection: Application to red blood cells and cultured fibroblasts. J. Chromatograp. B: Biomed. Sci. Applied, 752: 123-132. DOI: 10.1016/S0378-4347(00) 00534-X.
  • 4
    • 0025889028 scopus 로고
    • IL-10 inhibits cytokine production by activated macrophages
    • PMID: 1940369
    • Fiorentino, D.F., A. Zlotnik, T.R. Mosmann, M. Howard and A. O'Garra, 1991. IL-10 inhibits cytokine production by activated macrophages. J. Immunol., 147: 3815-3822. PMID: 1940369.
    • (1991) J. Immunol , vol.147 , pp. 3815-3822
    • Fiorentino, D.F.1    Zlotnik, A.2    Mosmann, T.R.3    Howard, M.4    O'Garra, A.5
  • 5
    • 0024408824 scopus 로고
    • Two types of mouse T helper cell, IV Th2 clones secrete a factor that inhibits cytokine production by Th1 clones
    • PMID: 2531194
    • Fiorentino, D.F., M.W. Bond and T.R. Mosmann, 1989. Two types of mouse T helper cell, IV Th2 clones secrete a factor that inhibits cytokine production by Th1 clones. J. Exp. Med., 170: 2081-2095. PMID: 2531194.
    • (1989) J. Exp. Med , vol.170 , pp. 2081-2095
    • Fiorentino, D.F.1    Bond, M.W.2    Mosmann, T.R.3
  • 6
    • 0027985632 scopus 로고
    • Status of glutathione during oxidant induced oxidative stress in the preimplantation mouse embryo
    • PMID: 7888510
    • Gardiner, C.S. and D.J. Reed, 1994. Status of glutathione during oxidant induced oxidative stress in the preimplantation mouse embryo. Biol. Reprod., 51: 1307-1314. PMID: 7888510.
    • (1994) Biol. Reprod , vol.51 , pp. 1307-1314
    • Gardiner, C.S.1    Reed, D.J.2
  • 7
    • 0037084991 scopus 로고    scopus 로고
    • Determination of intracellular glutathione and thiols by high performance liquid chromatography with a gold electrode at the femtomole level: Comparison with a spectroscopic assay
    • DOI:10.1016/S0304-4165(02)00152-6.PMID: 11960688
    • Hiraku, Y., M. Murata and S. Kawanishi, 2002. Determination of intracellular glutathione and thiols by high performance liquid chromatography with a gold electrode at the femtomole level: Comparison with a spectroscopic assay. Biochimica et Biophysica Acta-General Subjects, 1570: 47-52. DOI:10.1016/S0304-4165(02)00152-6.PMID: 11960688.
    • (2002) Biochimica et Biophysica Acta-General Subjects , vol.1570 , pp. 47-52
    • Hiraku, Y.1    Murata, M.2    Kawanishi, S.3
  • 8
    • 0032575366 scopus 로고    scopus 로고
    • Glutathione measurement in human plasma. Evaluation of sample collection, storage and derivatization conditions for analysis of dansyl derivatives by HPLC
    • PMID: 9721075, DOI: 10.1016/S0009-8981(98)00089-8
    • Jones, D.P., J.L. Carlson, P.S. Samiec, J.P. Sternberg, J.V.C. Mody, R.L. Reed and L.A. Brown, 1998. Glutathione measurement in human plasma. Evaluation of sample collection, storage and derivatization conditions for analysis of dansyl derivatives by HPLC. Clinica Chimica Acta, 275: 175-184. DOI: 10.1016/S0009-8981(98)00089-8. PMID: 9721075.
    • (1998) Clinica Chimica Acta , vol.275 , pp. 175-184
    • Jones, D.P.1    Carlson, J.L.2    Samiec, P.S.3    Sternberg, J.P.4    Mody, J.V.C.5    Reed, R.L.6    Brown, L.A.7
  • 9
    • 0033214665 scopus 로고    scopus 로고
    • Analysis of glutathione and glutathione disulfide in whole cells and mitochondria by postcolumn derivatization high-performance liquid chromatography with ortho-phthalaldehyde
    • PMID: 10527505, DOI: 10.1006/abio.1999.4258
    • Lenton, K.J., H. Therriault and J.R. Wagner, 1999. Analysis of glutathione and glutathione disulfide in whole cells and mitochondria by postcolumn derivatization high-performance liquid chromatography with ortho-phthalaldehyde. Analytical Biochem., 274: 125-130. DOI: 10.1006/abio.1999.4258. PMID: 10527505.
    • (1999) Analytical Biochem , vol.274 , pp. 125-130
    • Lenton, K.J.1    Therriault, H.2    Wagner, J.R.3
  • 10
    • 0033067354 scopus 로고    scopus 로고
    • Regulation of hepatic glutathione synthesis: Current concepts and controversies
    • PMID: 10385608
    • Lu, S.C., 1999. Regulation of hepatic glutathione synthesis: Current concepts and controversies. The FASEB J., 13: 1169-1183. PMID: 10385608.
    • (1999) The FASEB J , vol.13 , pp. 1169-1183
    • Lu, S.C.1
  • 11
    • 0027422313 scopus 로고
    • Dendritic cells and macrophages are required for Th1 development of CD4 T cells from alpha beta TCR transgenic mice: IL-12 substitution for macrophages to stimulate IFN-gamma production is IFN-gamma dependent
    • PMID: 7902129
    • Macatonia, S.E., C.S. Hsieh, K.M. Murphy and A. O'Garra, 1993. Dendritic cells and macrophages are required for Th1 development of CD4 T cells from alpha beta TCR transgenic mice: IL-12 substitution for macrophages to stimulate IFN-gamma production is IFN-gamma dependent. Int. Immunol., 5: 1119-1128. PMID: 7902129.
    • (1993) Int. Immunol , vol.5 , pp. 1119-1128
    • Macatonia, S.E.1    Hsieh, C.S.2    Murphy, K.M.3    O'Garra, A.4
  • 12
    • 0025797088 scopus 로고
    • Fluorimetric determination of oxidized and reduced glutathione in cells and tissues by high-performance liquid chromatography following derivatization with dansyl chloride
    • PMID: 1770099
    • Martin, J. and I.N. White, 1991. Fluorimetric determination of oxidized and reduced glutathione in cells and tissues by high-performance liquid chromatography following derivatization with dansyl chloride. J. Chromatogr. Biomed. Applic., 568 (1): 219-225. PMID: 1770099.
    • (1991) J. Chromatogr. Biomed. Applic , vol.568 , Issue.1 , pp. 219-225
    • Martin, J.1    White, I.N.2
  • 13
    • 0025744442 scopus 로고
    • Role of a new cytokine interleukin-10 in the cross regulation of T helper cells
    • PMID: 1648883
    • Mosmann, T.R., 1991. Role of a new cytokine interleukin-10 in the cross regulation of T helper cells. Ann. N.Y. Acad. Sci., 628: 337-344. PMID: 1648883.
    • (1991) Ann. N.Y. Acad. Sci , vol.628 , pp. 337-344
    • Mosmann, T.R.1
  • 14
    • 0037505644 scopus 로고    scopus 로고
    • Analysis of glutathione: Implication in redox and detoxification
    • DOI: 10.1016/S0009-8981(03)00200-6.PMID:12809732
    • Pastore, A., G. Federic, E. Bertini and F. Piemonte, 2003. Analysis of glutathione: Implication in redox and detoxification. Clinica Chimica Acta, 333: 19-39. DOI: 10.1016/S0009-8981(03)00200-6.PMID:12809732.
    • (2003) Clinica Chimica Acta , vol.333 , pp. 19-39
    • Pastore, A.1    Federic, G.2    Bertini, E.3    Piemonte, F.4
  • 15
    • 0019131878 scopus 로고
    • High-performance liquid chromatography analysis of nanomole levels of glutathione, glutathione disulfide and related thiols and disulfides
    • PMID: 7416469, DOI: 10.1016/0003-2697(80)90118-9
    • Reed, D.J., J.R. Babson, P.W. Beatty, A.E. Brodie, W.W. Ellis and D.W. Potter, 1980. High-performance liquid chromatography analysis of nanomole levels of glutathione, glutathione disulfide and related thiols and disulfides. Analyt. Biochem., 106: 55-62. DOI: 10.1016/0003-2697(80)90118-9. PMID: 7416469.
    • (1980) Analyt. Biochem , vol.106 , pp. 55-62
    • Reed, D.J.1    Babson, J.R.2    Beatty, P.W.3    Brodie, A.E.4    Ellis, W.W.5    Potter, D.W.6
  • 16
    • 0028978680 scopus 로고
    • Biology of human TH1 and TH2 cells
    • PMID: 7559914
    • Romagnani, S., 1995. Biology of human TH1 and TH2 cells. J. Clin. Immunol., 15: 121-129. PMID: 7559914.
    • (1995) J. Clin. Immunol , vol.15 , pp. 121-129
    • Romagnani, S.1
  • 17
    • 0022449812 scopus 로고
    • The role of oxidative processes in the cytotoxicity of substituted 1, 4-naphthoquinones in isolated hepatocytes
    • PMID: 3017211, DOI: 10.1016/0003-9861(86) 90499-6
    • Ross, D., H. Thor, M.D. Threadgill, M.S. Sandy, M.T. Smith, P. Moldeus and S. Orrenius, 1986. The role of oxidative processes in the cytotoxicity of substituted 1, 4-naphthoquinones in isolated hepatocytes. Arch. Biochem. Biophys., 248: 460-466. DOI: 10.1016/0003-9861(86) 90499-6. PMID: 3017211.
    • (1986) Arch. Biochem. Biophys , vol.248 , pp. 460-466
    • Ross, D.1    Thor, H.2    Threadgill, M.D.3    Sandy, M.S.4    Smith, M.T.5    Moldeus, P.6    Orrenius, S.7
  • 18
    • 0031545084 scopus 로고    scopus 로고
    • Nutritional biochemistry of cellular glutathione
    • DOI: 10.1016/S0955-2863(97)00113-7
    • Sen, C.K., 1997. Nutritional biochemistry of cellular glutathione. J. Nutr. Biochem., 8: 660-672. DOI: 10.1016/S0955-2863(97)00113-7.
    • (1997) J. Nutr. Biochem , vol.8 , pp. 660-672
    • Sen, C.K.1
  • 19
    • 0030787130 scopus 로고    scopus 로고
    • Use of sodium per chlorate at low pH for peptide separations by reversed-phase liquid chromatography. Influence of per chlorate ion on apparent hydrophilicity of positively charged amino acid side-chains
    • PMID: 9286089, DOI: 10.1016/S0021-9673(97)00150-7
    • Sereda, T.J., C.T. Mant and R.S. Hodges, 1997. Use of sodium per chlorate at low pH for peptide separations by reversed-phase liquid chromatography. Influence of per chlorate ion on apparent hydrophilicity of positively charged amino acid side-chains. J. Chromatogr. A, 776 (1): 153-165. DOI: 10.1016/S0021-9673(97)00150-7. PMID: 9286089.
    • (1997) J. Chromatogr. A , vol.776 , Issue.1 , pp. 153-165
    • Sereda, T.J.1    Mant, C.T.2    Hodges, R.S.3
  • 20
    • 0033230043 scopus 로고    scopus 로고
    • Glutathione and its role in cellular functions
    • PMID: 10569624, DOI: 10.1016/S0891-5849(99)00177-X
    • Sies, H., 1999. Glutathione and its role in cellular functions. Free Radical Biol. Med., 27: 916-921. DOI: 10.1016/S0891-5849(99)00177-X. PMID: 10569624.
    • (1999) Free Radical Biol. Med , vol.27 , pp. 916-921
    • Sies, H.1
  • 21
    • 0029891459 scopus 로고    scopus 로고
    • Determination of reduced and oxidized glutathione in erythrocytes by high performance liquid chromatography with ultraviolet absorbance dedection
    • PMID: 8738017
    • Yoshida, T., 1996. Determination of reduced and oxidized glutathione in erythrocytes by high performance liquid chromatography with ultraviolet absorbance dedection. J. Chromatogr. B-Biomed. Applic., 678 (2): 157-164. PMID: 8738017.
    • (1996) J. Chromatogr. B-Biomed. Applic , vol.678 , Issue.2 , pp. 157-164
    • Yoshida, T.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.