Genotyping of different Pseudomonas aeruginosa morphotypes arising from the lower respiratory tract of a patient taken to an Intensive Care Unit

International Journal of Immunopathology and Pharmacology

Volumn 21, Issue 4, 2008, Pages 941-947

  Putignani, L ^a   Sessa, R ^b   Petrucca, A ^c,d   Manfredini, C ^a   Coltella, L ^a   Menichella, D ^a   Nicoletti, Mauro ^d   Russo, C ^a   Cipriani, P ^c  

^a BAMBINO GESÙ CHILDREN S HOSPITAL   (Italy)

^b SAPIENZA UNIVERSITY OF ROME   (Italy)

^c SAPIENZA UNIVERSITY OF ROME   (Italy)

^d UNIVERSITY OF CHIETI   (Italy)

Author keywords

Colony variant; f AFLP; Genome plasticity; Pseudomonas aeruginosa; rep PCR fingerprinting

Indexed keywords

AMIKACIN; AZTREONAM; CEFEPIME; CEFTAZIDIME; CIPROFLOXACIN; GENTAMICIN; IMIPENEM; MEROPENEM; MEZLOCILLIN; NETILMICIN; PIPERACILLIN PLUS TAZOBACTAM;

AMPLIFIED FRAGMENT LENGTH POLYMORPHISM; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL COLONIZATION; BACTERIAL GROWTH; BACTERIUM COLONY; BACTERIUM CULTURE; BACTERIUM ISOLATE; BACTERIUM ISOLATION; CONTROLLED STUDY; CULTURE MEDIUM; GENOTYPE; INTENSIVE CARE UNIT; LENGTH OF STAY; LOWER RESPIRATORY TRACT; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA;

EID: 59849100599     PISSN: 03946320     EISSN: None     Source Type: Journal    
DOI: 10.1177/039463200802100418     Document Type: Article

References (24)

1. Aliaga L, Mediavilla JD, Cobo F. A clinical index predicting mortality with Pseudomonas aeruginosa bacteraemia. J Med Microbiol 2002; 51:615-19.
2. Denervaud V, TuQuoc P, Blanc D, Favre-Bonte S, Krishnapillai V, Reimmann C, Haas D, van Delden C. Characterization of cell-to-cell signaling-deficient Pseudomonas aeruginosa strains colonizing intubated patients. J Clin Microbiol 2004; 42:554-62.
3. Diaz E, Munoz E, Agbaht K, Rello J. Management of ventilator associated pneumonia caused by multiresistant bacteria. Curr Opin Crit Care 2007; 13:45-50.
4. Pirnay JP, De Vos D, Cochez C, Bilocq F, Pirson J, Struelens M, Duinslaeger L, Cornelis P, Zizi M, Vanderkelen A. Molecular epidemiology of Pseudomonas aeruginosa colonization in a burn unit: persistence of a multidrug-resistant clone and a silver sulfadiazine-resistant clone. J Clin Microbiol 2003; 41:1192-202.
5. Oliver A, Canton R, Campo P, Baquero F, Blazquez J. High frequency of hypermutable Pseudomonas aeruginosa in cystic fibrosis lung infection. Science 2000; 288:1251-4.
6. Silbert S, Barth AL, Sader HS. Heterogeneity of Pseudomonas aeruginosa in brazilian cystic fibrosis patients. J Clin Microbiol 2001; 39:3976-81.
7. Struelens MJ, Schwam V, Deplano A, Baran D. Genome macrorestriction analysis of diversity and variability of Pseudomonas aeruginosa strains infecting cystic fibrosis patients. J Clin Microbiol 1993; 31:2320-6.
8. Tummler B. Clonal variations in Pseudomonas aeruginosa. In: Pseudomonas. Molecular biology of emerging issues. Ramos J., Levesque L., ed. Springer Now York, 2006; p. 35.
9. Zierdt CH, Schmidt PJ. Dissociation in Pseudomonas aeruginosa. J Bacteriol 1964; 87:1003-10.
10. Finnan S, Morrissey JP, O'Gara F, Boyd EF. Genome diversity of Pseudomonas aeruginosa isolates from cystic fibrosis patients and the hospital environment. J Clin Microbiol 2004; 42:5783-92.
11. Gastmeier P, Schwabb F, Bärwolffb S, Rüdenb H, Grundmannc H. Correlation between the genetic diversity of nosocomial pathogens and their survival time in intensive care units. J Hosp Infect. 2006; 62: 181-6.
12. Head NE, Yu H. Cross-sectional analysis of clinical and environmental isolates of Pseudomonas aeruginosa: biofilm formation, virulence, and genome diversity. Infect Immun 2004; 72:133-44.
13. Lanotte P, Watt S, Mereghetti L, Dartiguelongue N, Rastegar-Lari A, Goudeau A, Quentin R. Genetic features of Pseudomonas aeruginosa isolates from cystic fibrosis patients compared with those of isolates from other origins. J Med Microbiol 2004; 53:73-81.
14. Liang X, Pham XT, Olson MV, Lory S. Identification of a genomic island present in the majority of pathogenic isolates of Pseudomonas aeruginosa. J Bacteriol 2001; 183:843-53.
15. Ruimy R, Genauzeau E, Barnabe C, Beaulieu A, Tibayrenc M, Andremont A. Genetic diversity of Pseudomonas aeruginosa strains isolated from ventilated patients with nosocomial pneumonia, cancer patients with bacteremia, and environmental water. Infect Immun 2001; 69:584-8.
16. Spencer DH, Kas A, Smith EE, Raymond CK, Sims EH, Hastings M, Bums JL, Kaul R, Olson MV. Whole-genome sequence variation among multiple isolates of Pseudomonas aeruginosa. J. Bacteriol. 2003; 185:1316-25.
17. Stover CK, Pham XQ, Erwin AL, et al. Complete genome sequence of Pseudomonas aeruginosa PA01, an opportunistic pathogen. Nature 2000; 406:959-64.
18. Häußler S, Ziegler I, Löttel A, von Götz F, Rohde M, Wehmhöhner D, Saravanamuthu S, Tümmler B, Steinmetz I. Highly adherent small-colony variants of Pseudomonas aeruginosa in cystic fibrosis lung infection. J Med Microbiol 2003; 52:295-301.
19. Harrington SM, Stock F, Kominski AL, Campbell JD, Hormazabal JC, Livio S, Rao L, Kotloff KL, Sow SO, Murray PR. Genotypic Analysis of Invasive Streptococcus pneumoniae from Mali, Africa, by Semiautomated Repetitive-Element PCR and Pulsed-Field Gel Electrophoresis. J Clin Microbiol 2007; 45:707-14.
20. Ross TL, Merz WG, Farkosh M, Carroll KC. Comparison of an Automated Repetitive Sequence-Based PCR Microbial Typing System to Pulsed-Field Gel Electrophoresis for analysis of outbreaks of Methicillin-Resistant Staphylococcus aureus. J Clin Microbiol 2005; 43:5642-7.
21. Speijer H, Savelkoul PHM, Bonten MJ, Stobberingh EE, Tjhie JHT. Application of different genotyping methods for Pseudomonas aeruginosa in a setting of endemicity in an intensive care unit. J Clin Microbiol 1999; 37:3654-61.
22. Fonseca AP, Correia P, Sousa JC, Tenreiro R. Association patterns of Pseudomonas aeruginosa clinical isolates as revealed by virulence traits, antibiotic resistance, serotype and genotype. FEMS Immunol Med Microbiol 2007; 51:505-16.
23. Lomholt JA, Poulsen K, Kiliani M. Epidemic population structure of Pseudomonas aeruginosa: evidence for a clone that is pathogenic to the eye and that has a distinct combination of virulence factors. Infect Immun 2001; 69:6284-95.
24. von Götz F, Häussler S, Jordan D, Saravanamuthu S, Wehmhöner D, Strüßmann A, Lauber S, Attree I, Tümmler JBB, Steinmetz I. Expression analysis of a highly adherent and cytotoxic small colony variant of Pseudomonas aeruginosa isolated from a lung of a patient with cystic fibrosis. J Bacteriol 2004; 186: 3837-43.