Real-time live imaging to study bacterial infections in vivo

Current Opinion in Microbiology

Volumn 12, Issue 1, 2009, Pages 31-36

  Melican, Keira ^a   Richter Dahlfors, Agneta ^a  

^a KAROLINSKA INSTITUTE   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL INFECTIONS; BACTERIAL PHYSIOLOGICAL PHENOMENA; BACTERIAL PROTEINS; HOST-PATHOGEN INTERACTIONS; IMAGE PROCESSING, COMPUTER-ASSISTED; MICROSCOPY; VIRULENCE FACTORS;

BACTERIA (MICROORGANISMS);

EID: 59849091723     PISSN: 13695274     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mib.2008.11.002     Document Type: Review

References (48)

1. Coburn B., Sekirov I., and Finlay B.B. Type III secretion systems and disease. Clin Microbiol Rev 20 (2007) 535-549
2. Dean P., Maresca M., and Kenny B. EPEC's weapons of mass subversion. Curr Opin Microbiol 8 (2005) 28-34
3. Phalipon A., and Sansonetti P.J. Shigella's ways of manipulating the host intestinal innate and adaptive immune system: a tool box for survival?. Immunol Cell Biol 85 (2007) 119-129
4. Egen J.G., Rothfuchs A.G., Feng C.G., Winter N., Sher A., and Germain R.N. Macrophage and T cell dynamics during the development and disintegration of mycobacterial granulomas. Immunity 28 (2008) 271-284. This study describes live intravital multiphoton microscopy of Mycobacterium bovis BCG-induced liver granulomas and the migration of both innate and adaptive immune cells throughout the process of granuloma development.
5. Mansson L.E., Melican K., Boekel J., Sandoval R.M., Hautefort I., Tanner G.A., Molitoris B.A., and Richter-Dahlfors A. Real-time studies of the progression of bacterial infections and immediate tissue responses in live animals. Cell Microbiol 9 (2007) 413-424. This paper reports the first use of multiphoton microscopy to follow the progression of a bacterial infection in a live animal. Describes the first hours of kidney infection with uropathogenic Escherichia coli.
6. Melican K., Boekel J., Mansson L.E., Sandoval R.M., Tanner G.A., Kallskog O., Palm F., Molitoris B.A., and Richter-Dahlfors A. Bacterial infection-mediated mucosal signalling induces local renal ischaemia as a defence against sepsis. Cell Microbiol 10 (2008) 1987-1998. Multiphoton imaging of live bacterial infection identifies ischemia as an innate immune response to renal infection. This response is shown to protect against sepsis.
7. Richter Dahlfors A.A., and Kurland C.G. Novel mutants of elongation factor G. J Mol Biol 215 (1990) 549-557
8. Deng W., Puente J.L., Gruenheid S., Li Y., Vallance B.A., Vazquez A., Barba J., Ibarra J.A., O'Donnell P., Metalnikov P., et al. Dissecting virulence: systematic and functional analyses of a pathogenicity island. Proc Natl Acad Sci U S A 101 (2004) 3597-3602
9. Lecuit M., Vandormael-Pournin S., Lefort J., Huerre M., Gounon P., Dupuy C., Babinet C., and Cossart P. A transgenic model for listeriosis: role of internalin in crossing the intestinal barrier. Science 292 (2001) 1722-1725
10. Richter-Dahlfors A., Buchan A.M., and Finlay B.B. Murine salmonellosis studied by confocal microscopy: Salmonella typhimurium resides intracellularly inside macrophages and exerts a cytotoxic effect on phagocytes in vivo. J Exp Med 186 (1997) 569-580
11. Tam V.C., Serruto D., Dziejman M., Brieher W., and Mekalanos J.J. A type III secretion system in Vibrio cholerae translocates a formin/spire hybrid-like actin nucleator to promote intestinal colonization. Cell Host Microbe 1 (2007) 95-107
12. Germain R.N., Miller M.J., Dustin M.L., and Nussenzweig M.C. Dynamic imaging of the immune system: progress, pitfalls and promise. Nat Rev Immunol 6 (2006) 497-507
13. Contag C.H., Contag P.R., Mullins J.I., Spilman S.D., Stevenson D.K., and Benaron D.A. Photonic detection of bacterial pathogens in living hosts. Mol Microbiol 18 (1995) 593-603
14. Orihuela C.J., Gao G., McGee M., Yu J., Francis K.P., and Tuomanen E. Organ-specific models of Streptococcus pneumoniae disease. Scand J Infect Dis 35 (2003) 647-652
15. Kadurugamuwa J.L., Modi K., Yu J., Francis K.P., Purchio T., and Contag P.R. Noninvasive biophotonic imaging for monitoring of catheter-associated urinary tract infections and therapy in mice. Infect Immun 73 (2005) 3878-3887
16. Hutchens M., and Luker G.D. Applications of bioluminescence imaging to the study of infectious diseases. Cell Microbiol 9 (2007) 2315-2322
17. Doyle T.C., Burns S.M., and Contag C.H. In vivo bioluminescence imaging for integrated studies of infection. Cell Microbiol 6 (2004) 303-317
18. Enninga J., Sansonetti P., and Tournebize R. Roundtrip explorations of bacterial infection: from single cells to the entire host and back. Trends Microbiol 15 (2007) 483-490
19. Sheth V.R., van Heeckeren R.C., Wilson A.G., van Heeckeren A.M., and Pagel M.D. Monitoring infection and inflammation in murine models of cystic fibrosis with magnetic resonance imaging. J Magn Reson Imaging 28 (2008) 527-532
20. Tournebize R., Doan B.T., Dillies M.A., Maurin S., Beloeil J.C., and Sansonetti P.J. Magnetic resonance imaging of Klebsiella pneumoniae-induced pneumonia in mice. Cell Microbiol 8 (2006) 33-43
21. Lankinen P., Makinen T.J., Poyhonen T.A., Virsu P., Salomaki S., Hakanen A.J., Jalkanen S., Aro H.T., and Roivainen A. (68)Ga-DOTAVAP-P1 PET imaging capable of demonstrating the phase of inflammation in healing bones and the progress of infection in osteomyelitic bones. Eur J Nucl Med Mol Imaging 35 (2008) 352-364
22. Schuster D.P., Kozlowski J., and Hogue L. Imaging lung inflammation in a murine model of Pseudomonas infection: a positron emission tomography study. Exp Lung Res 29 (2003) 45-57
23. Tarun A.S., Baer K., Dumpit R.F., Gray S., Lejarcegui N., Frevert U., and Kappe S.H. Quantitative isolation and in vivo imaging of malaria parasite liver stages. Int J Parasitol 36 (2006) 1283-1293
24. Amino R., Thiberge S., Blazquez S., Baldacci P., Renaud O., Shorte S., and Menard R. Imaging malaria sporozoites in the dermis of the mammalian host. Nat Protoc 2 (2007) 1705-1712
25. Thiberge S., Blazquez S., Baldacci P., Renaud O., Shorte S., Menard R., and Amino R. In vivo imaging of malaria parasites in the murine liver. Nat Protoc 2 (2007) 1811-1818
26. Svoboda K., and Yasuda R. Principles of two-photon excitation microscopy and its applications to neuroscience. Neuron 50 (2006) 823-839
27. Celli S., and Bousso P. Intravital two-photon imaging of T-cell priming and tolerance in the lymph node. Methods Mol Biol 380 (2007) 355-363
28. Ashworth S.L., Sandoval R.M., Tanner G.A., and Molitoris B.A. Two-photon microscopy: visualization of kidney dynamics. Kidney Int 72 (2007) 416-421. Describes multiphoton-based methods to visualize dynamics of physiological events in vivo.
29. Mansson L.E., Melican K., Molitoris B.A., and Richter-Dahlfors A. Progression of bacterial infections studied in real time-novel perspectives provided by multiphoton microscopy. Cell Microbiol 9 (2007) 2334-2343
30. Bullen A. Microscopic imaging techniques for drug discovery. Nat Rev Drug Discov 7 (2008) 54-67
31. Helmchen F., and Denk W. Deep tissue two-photon microscopy. Nat Methods 2 (2005) 932-940. Excellent review of the principles behind multiphoton microscopy in various tissues.
32. Piston D.W. Imaging living cells and tissues by two-photon excitation microscopy. Trends Cell Biol 9 (1999) 66-69
33. Chieppa M., Rescigno M., Huang A.Y., and Germain R.N. Dynamic imaging of dendritic cell extension into the small bowel lumen in response to epithelial cell TLR engagement. J Exp Med 203 (2006) 2841-2852
34. Chtanova T., Schaeffer M., Han S.J., van Dooren G.G., Nollmann M., Herzmark P., Chan S.W., Satija H., Camfield K., Aaron H., et al. Dynamics of neutrophil migration in lymph nodes during infection. Immunity 29 (2008) 487-496
35. Aoshi T., Zinselmeyer B.H., Konjufca V., Lynch J.N., Zhang X., Koide Y., and Miller M.J. Bacterial entry to the splenic white pulp initiates antigen presentation to CD8+ T cells. Immunity 29 (2008) 476-486
36. Zinselmeyer B.H., Lynch J.N., Zhang X., Aoshi T., and Miller M.J. Video-rate two-photon imaging of mouse footpad - a promising model for studying leukocyte recruitment dynamics during inflammation. Inflamm Res 57 (2008) 93-96. Uses multiphoton microscopy to describe the behavior of neutrophils at the site of a live L. monocytogenes infection in a mouse footpad.
37. Hautefort I., Proenca M.J., and Hinton J.C. Single-copy green fluorescent protein gene fusions allow accurate measurement of Salmonella gene expression in vitro and during infection of mammalian cells. Appl Environ Microbiol 69 (2003) 7480-7491
38. 2+ oscillations in renal epithelial cells. Nature 405 (2000) 694-697
39. Junt T., Moseman E.A., Iannacone M., Massberg S., Lang P.A., Boes M., Fink K., Henrickson S.E., Shayakhmetov D.M., Di Paolo N.C., et al. Subcapsular sinus macrophages in lymph nodes clear lymph-borne viruses and present them to antiviral B cells. Nature 450 (2007) 110-114
40. Sutton T.A., Mang H.E., Campos S.B., Sandoval R.M., Yoder M.C., and Molitoris B.A. Injury of the renal microvascular endothelium alters barrier function after ischemia. Am J Physiol Renal Physiol 285 (2003) F191-198
41. Bao H., Allen J., Pattie R., Vance R., and Gu M. Fast handheld two-photon fluorescence microendoscope with a 475 microm × 475 microm field of view for in vivo imaging. Opt Lett 33 (2008) 1333-1335
42. Konig K., Ehlers A., Riemann I., Schenkl S., Buckle R., and Kaatz M. Clinical two-photon microendoscopy. Microsc Res Tech 70 (2007) 398-402
43. Rogart J.N., Nagata J., Loeser C.S., Roorda R.D., Aslanian H., Robert M.E., Zipfel W.R., and Nathanson M.H. Multiphoton imaging can be used for microscopic examination of intact human gastrointestinal mucosa ex vivo. Clin Gastroenterol Hepatol 6 (2008) 95-101
44. Sayeed S., Uzal F.A., Fisher D.J., Saputo J., Vidal J.E., Chen Y., Gupta P., Rood J.I., and McClane B.A. Beta toxin is essential for the intestinal virulence of Clostridium perfringens type C disease isolate CN3685 in a rabbit ileal loop model. Mol Microbiol 67 (2008) 15-30
45. Wang T.D., and Triadafilopoulos G. Autofluorescence imaging: have we finally seen the light?. Gastrointest Endosc 61 (2005) 686-688
46. Dunn K.W., Sandoval R.M., Kelly K.J., Dagher P.C., Tanner G.A., Atkinson S.J., Bacallao R.L., and Molitoris B.A. Functional studies of the kidney of living animals using multicolor two-photon microscopy. Am J Physiol Cell Physiol 283 (2002) C905-C916
47. Russo L.M., Sandoval R.M., McKee M., Osicka T.M., Collins A.B., Brown D., Molitoris B.A., and Comper W.D. The normal kidney filters nephrotic levels of albumin retrieved by proximal tubule cells: retrieval is disrupted in nephrotic states. Kidney Int 71 (2007) 504-513
48. Sandoval R.M., and Molitoris B.A. Quantifying endocytosis in vivo using intravital two-photon microscopy. Methods Mol Biol 440 (2008) 389-402. Describes the use of multiphoton imaging for high sensitivity quantification of endocytosis events in the live kidney with superior spatial and temporal resolution.