An enzyme cocktail for efficient protoplast formation in Aspergillus niger

Journal of Microbiological Methods

Volumn 76, Issue 3, 2009, Pages 305-306

  de Bekker, Charissa ^a   Wiebenga, Ad ^a   Aguilar, Guillermo ^a   Wösten, Han A B ^a  

^a UTRECHT UNIVERSITY   (Netherlands)

Author keywords

Aspergillus niger; Fungi; Protoplast formation; Protoplast regeneration; Protoplast storage; Protoplast transformation

Indexed keywords

BETA GLUCURONIDASE; CHITINASE; FUNGAL ENZYME;

ARTICLE; ASPERGILLUS NIGER; CELL TRANSFORMATION; CHEMICAL COMPOSITION; CULTURE MEDIUM; FUNGUS CULTURE; HELIX POMATIA; NONHUMAN; PRIORITY JOURNAL; PROTOPLAST CULTURE; STREPTOMYCES GRISEUS; TRICHODERMA HARZIANUM;

ANIMALS; ASPERGILLUS NIGER; CHITINASE; GLUCURONIDASE; HELIX (SNAILS); PROTOPLASTS; STREPTOMYCES GRISEUS; TRICHODERMA;

ASPERGILLUS NIGER; FUNGI; HELIX POMATIA; HYPOCREA LIXII; STREPTOMYCES GRISEUS;

EID: 59849087031     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2008.11.001     Document Type: Article

References (11)

1. Dawe A.L., Willins D.A., and Morris N.R. Increased transformation efficiency of Aspergillus nidulans protoplasts in the presence of dithiothreitol. Anal. Biochem. 283 (2000) 111-112
2. Evans C.T., and Conrad D. An improved method for protoplast formation and its application in the fusion of Rhodotorula rubra with Saccharomyces cerevisiae. Arch. Microbiol. 148 (1987) 77-82
3. Goosen T., Bloemheuvel G., Gysler C., de Bie D.A., van den Broek H.W., and Swart K. Transformation of Aspergillus niger using the homologous orotidine-5'-phosphate-decarboxylase gene. Curr. Genet. 11 (1987) 499-503
4. Gouka R.J., Gerk C., Hooykaas P.J.J., Bundock P., Musters W., Verrips C.T., and de Groot M.J. Transformation of Aspergillus awamori by Agrobacterium tumefaciens-mediated homologous recombination. Nat. Biotechnol. 17 (1999) 598-601
5. Jung M.K., Ovechkina Y., Prigozhina N., Oakley C.E., and Oakley B.R. The use of beta-d-glucanase as a substitute for Novozym 234 in immunofluorescence and protoplasting. Fungal Genet. Newslett. 47 (2000) 65-66
6. Kusters-van Someren M.A., Harmsen J.A., Kester H.C., and Visser J. Structure of the Aspergillus niger pelA gene and its expression in Aspergillus niger and Aspergillus nidulans. Curr. Genet. 20 (1991) 293-299
7. Meyer V., Mueller D., Strowig T., and Stahl U. Comparison of different transformation methods for Aspergillus giganteus. Curr. Genet. 43 (2003) 371-377
8. Michielse C.B., Arentshorst M., Ram A.F.J., and van den Hondel C.A.M.J.J. Agrobacterium-mediated transformation leads to improved gene replacement efficiency in Aspergillus awamori. Fungal Genet. Biol. 42 (2005) 9-19
9. Peraza L., Ortiz M.A., Peberdy J.F., and Aguilar G. Growth and pectinase production by Aspergillus Mexican strain protoplast regenerated under acidic stress. Appl. Biochem. Biotechnol. 111 (2003) 15-27
10. Punt P.J., Oliver R.P., Dingemanse M.A., Pouwels P.H., and van den Hondel C.A.M.J.J. Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli. Gene 56 (1987) 117-124
11. Sugui J.A., Chang Y.C., and Kwon-Chung K.J. Agrobacterium tumefaciens-mediated transformation of Aspergillus fumigatus: an efficient tool for insertional mutagenesis and targeted gene disruption. Appl. Environ. Microbiol. 71 (2005) 1798-1802