Bone marrow-derived mesenchymal stem cells: Isolation, expansion, characterization, viral transduction, and production of conditioned medium

Methods in Molecular Biology

Volumn 482, Issue , 2009, Pages 281-294

  Gnecchi, Massimiliano ^a   Melo, Luis G ^a  

^a NONE

Author keywords

Cell surface markers; Conditioned medium; Drug discovery; Flow cytometry; Gene transfer; Mesenchymal stem cells; Paracrine effect; Soluble factors

Indexed keywords

ANIMAL; ARTICLE; BONE MARROW CELL; C57BL MOUSE; CELL CULTURE; CELL PROLIFERATION; CELL SEPARATION; CULTURE MEDIUM; CYTOLOGY; FLUORESCENCE MICROSCOPY; GENETIC TRANSDUCTION; GENETICS; IMMUNOPHENOTYPING; MALE; MESENCHYMAL STEM CELL; METHODOLOGY; MOUSE; RETROVIRUS;

ANIMALS; BONE MARROW CELLS; CELL PROLIFERATION; CELL SEPARATION; CELLS, CULTURED; CULTURE MEDIA, CONDITIONED; IMMUNOPHENOTYPING; MALE; MESENCHYMAL STEM CELLS; MICE; MICE, INBRED C57BL; MICROSCOPY, FLUORESCENCE; RETROVIRIDAE; TRANSDUCTION, GENETIC;

EID: 59749100262     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-060-7_18     Document Type: Article

References (32)

1. Dexter, T.M., Allen, T.D., Lajtha, L.G. (1977) Conditions controlling the proliferation of haemopoietic stem cells in vitro. J Cell Physiol 91, 335-344.
2. Becker, A.J., McCulloch, E.A., Till, J.E. (1963) Cytological demonstration of the clonal nature of spleen colonies derived from transplanted mouse marrow cells. Nature 197, 452-454.
3. Siminovitch, L., McCulloch, E.A., Till, J.E. (1963) The distribution of colony-forming cells among spleen colonies. J Cell Physiol 62, 327-336.
4. Friedenstein, A.J., Chailakhjan, R.K., Lalykina, K.S. (1970) The development of fibroblast colonies in monolayer cultures of guinea pig bone marrow and spleen cells. Cell Tissue Kinet 3, 393-403.
5. Friedenstein, A.J., Deriglasova, U.F., Kulagina, N.N., Panasuk, A.F., Rudakowa, S.F., Luria, E.A., Ruadkow, I.A. (1974) Precursors for fibroblasts in different populations of hematopoietic cells as detected by the in vitro colony assay method. Exp Hematol 2, 83-92.
6. Friedenstein, A.J., Chailakhyan, R.K., Gerasimov, U.V. (1987) Bone marrow osteogenic stem cells: in vitro cultivation and transplantation in diffusion chambers. Cell Tissue Kinet 20, 263-272.
7. Aston, B.A., Allen, T.D., Howlett, C.R., Eaglesom, C.C., Hattori, A., Owen, M. (1980) Formation of bone and cartilage by marrow stromal cells in diffusion chambers in vivo. Clin Orthop Relat Res 151, 294-307.
8. Owen, M. (1988) Marrow stromal stem cells. J Cell Sci Suppl 10, 63-76.
9. Caplan, A.I. (1991) Mesenchymal stem cells. J Orthop Res 9, 641-650.
10. Minguelll, J.J., Erices, A., Conget, P. (2001) Mesenchymal stem cells. Exp Biol Med 226, 507-520.
11. He, Q., Wan, C., Li, G. (2007) Concise review: multipotent mesenchymal stromal cells in blood. Stem cells 25, 69-77.
12. Prockop, D.J. (1997) Marrow stromal cells as stem cells for nonhematopoietic tissues. Science 276, 71-74.
13. Nuttall, M.E., Patron, A.J., Olivera, D.L., Nadeau, D.P., Gowen, M. (1998) Human trabecular bone cells are able to express both osteoblastic and adipocytic phenotype; implications for osteopenic disorders. J Bone Miner Res 13, 371-382.
14. Wakitani, S., Saito, T., Caplan, A.I. (1995) Myogenic cells derived from rat bone marrow mesenchymal stem cells exposed to 5-azacytidine. Muscle Nerve 18, 1417-1426.
15. Kopen, G.G., Prockop, D.J., Phinney, D.G. (1999) Marrow stromal cells migrate throughout forebrain and cerebellum, and they differentiate into astrocytes after injection into neonatal mouse brains. Proc Natl Acad Sci U S A 96, 10711-10716.
16. Makino, S., Fukuda, K., Miyoshi, S., Kodama, H., Pan, J., Sano, M., Takahashi, T., Hori, S., Abe, H., Hata, J., Umezawa, A., Ogawa, S. (1999) Cardiomyocytes can be generated from marrow stromal cells in vitro. J Clin Invest 103, 697-705.
17. Pittenger, M.F., Mackay, A.M., Beck, S.C., Jaiswal, R.K., Douglas, R., Mosca, J.D., Moorman, M.A., Limoneti, D.W., Carig, S., Marshak, D.R (1999) Multilineage potential of adult human mesenchymal stem cells. Science 284, 143-147.
18. Bianco, P., Riminucci, M., Kuznetsov, S., and Robey, P.G. (1999) Multipotential cells in the bone marrow stroma: regulation in the context of organ physiology. Crit Rev Eukaryot Gene Exp 9, 159-173.
19. Liechty, K.W., MacKenzie, T.C., Shaaban, A.F., Radu, A., Moseley, A. M., Deans, R., Marshak, D. R., and Flake, A. W. (2000) Human mesenchymal stem cells engraft and demonstrate site-specific differentiation after in utero transplantation in sheep. Nat Med 6, 1282-1286.
20. Pochampally, R.R., Neville, B.T., Schwarz, E.J., Li, M. M., and Prockop, D. J. (2004) Rat adult stem cells (marrow stromal cells) engraft and differentiate in chick embryos without evidence of cell fusion. Proc Natl Acad Sci USA 101, 9282-9285.
21. Horwitz, E.M., Prockop, D.J., Fitzpatrick, L.A., Koo, W.W., Gordon, P.L., Neel, M., (1999) Transplantability and therapeutic effects of bone marrow-derived mesenchymal cells in children with osteogenesis imperfecta, Nat Med 5, 262-264.
22. Caplan A.I., Bruden, S.P. (2001) Mesenchymal stem cells: building blocks for molecular medicine in the 21th century. Trends Mol Med 7, 259-264.
23. Melo, L.G., Pachori, A.S., Kong, D., Gnecchi, M., Wang, K., Pratt, R. E., and Dzau, V. J. (2004) Molecular and cell-based therapies for protection, rescue, and repair of ischemic myocardium: reasons for cautious optimism. Circulation 109, 2386-2393.
24. Ryan, J.M., Barry, F.P., Murphy, J.M., Mahorn, B.P. (2005) Mesenchymal stem cells avoid allogeneic rejection, J Inflamm 26, 2-8.
25. Dzau, V.J., Gnecchi, M., Pachori, A.S. (2005) Enhancing stem cell therapy through genetic modification. J. Am Coll Cardiol 46, 1351-1353.
26. Caplan, A.I., Dennis, J.E. (2006) Mesenchymal Stem Cells as Trophic Mediators. J Cell Biochem 98, 1076-1084.
27. Gnecchi, M., He, H., Liang, O.D., Melo, L. G., Morello, F., Mu, H., Noiseux, N., Zhang, L., Pratt, R. E., Ingwall, J. S., et al. (2005) Paracrine action accounts for marked protection of ischemic heart by Akt-modified mesenchymal stem cells. Nat Med 11, 367-368.
28. Gnecchi, M., He, H., Noiseux, N., Liang, O. D., Zhang, L., Morello, F., Mu, H., Melo, L. G., Pratt, R. E., Ingwall, J. S., Dzau, V. J. (2006) Evidence supporting paracrine hypothesis for Akt-modified mesenchymal stem cell-mediated cardiac protection and functional improvement. FASEB J 20, 661-669.
29. Noiseux, N., Gnecchi, M., Lopez-Ilasca, M., Zhang, L., Solomon, S.D., Deb, A., Dzau, V.J., Pratt, R.E. (2006) Mesenchymal stem cells overexpressing Akt dramatically repair infarcted myocardium and improve cardiac function despite infrequent cellular fusion or differentiation. Mol Ther 14, 840-850.
30. Iso, Y., Spees, J.L., Serrano, C., Bakondi, B., Pochampally, R., Song, Y.H., Sobel, B.E., Delafontaine, P., Prockop, D.J. (2007) Multipotent human stromal cells improve cardiac function after myocardial infarction in mice without long-term engraftment. Biochem Biophys Res Commun 354, 700-706.
31. Honma, T., Honmou, O., Iihoshi, S., Harada, K., Houkin, K., Hamada, H., Kocsis, J. D. (2005) Intravenous infusion of immortalized human mesenchymal stem cells protects against injury in a cerebral ischemia model in adult rat. Exp Neurol 199, 56-66.
32. Togel, F., Weiss, K., Yang, Y., Hu, Z., Zhang, P., Westenfelder, C. (2007) Vasculotropic, paracrine actions of infused mesenchymal stem cells are important to the recovery from acute kidney injury. Am J Physiol Renal Physiol 292, F1626-F1635.