Mutational studies of archaeal RNA polymerase and analysis of hybrid RNA polymerases

Biochemical Society Transactions

Volumn 37, Issue 1, 2009, Pages 18-22

  Thomm, Michael ^a   Reich, Christoph ^a   Grünberg, Sebastian ^a   Naji, Souad ^a  

^a UNIVERSITY OF REGENSBURG   (Germany)

Author keywords

Archaeon; RNA polymerase; Transcription; Transcription factor E (TFE)

Indexed keywords

HETERODUPLEX; RNA POLYMERASE; RNA POLYMERASE II; RNA POLYMERASE SUBUNIT H; RNA POLYMERASE SUBUNIT P; RNA POLYMERASE SUBUNIT RPB12; RNA POLYMERASE SUBUNIT RPB5; SMALL SUBUNIT RIBOSOMAL RNA; TATA BINDING PROTEIN; TRANSCRIPTION FACTOR II; TRANSCRIPTION FACTOR IIB; TRANSCRIPTION FACTOR IIE; UNCLASSIFIED DRUG; ARCHAEAL PROTEIN; DNA DIRECTED RNA POLYMERASE; PROTEIN SUBUNIT; RECOMBINANT PROTEIN; TRANSCRIPTION FACTOR TFIIE;

AMINO TERMINAL SEQUENCE; ARCHAEAL GENOME; ARTICLE; COMPLEX FORMATION; ENZYME LOCALIZATION; ENZYME RECONSTITUTION; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; HETERODUPLEX ANALYSIS; MUTATIONAL ANALYSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN FUNCTION; RNA ANALYSIS; RNA TRANSLATION; TRANSCRIPTION INITIATION; AMINO ACID SEQUENCE; ARCHAEBACTERIUM; CHEMISTRY; ENZYMOLOGY; EUKARYOTIC CELL; GENETIC TRANSCRIPTION; GENETICS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE;

ARCHAEA; EUKARYOTA;

AMINO ACID SEQUENCE; ARCHAEA; ARCHAEAL PROTEINS; DNA MUTATIONAL ANALYSIS; DNA-DIRECTED RNA POLYMERASES; EUKARYOTIC CELLS; MOLECULAR SEQUENCE DATA; PROTEIN SUBUNITS; RECOMBINANT PROTEINS; TRANSCRIPTION FACTORS, TFII; TRANSCRIPTION, GENETIC;

EID: 59749087477     PISSN: 03005127     EISSN: 14708752     Source Type: Journal    
DOI: 10.1042/BST0370018     Document Type: Article

References (23)

1. Zillig, W., Stetter, K.O., Schnabel, R., Madon, J. and Gierl, A. (1982) Transcription in archaebacteria. Zbl. Bakt. Hyg. I. Abt. Orig. C3, 218-227
2. Goede, B., Naji, S., von Kampen, O., Ilg, K. and Thomm, M. (2006) Protein-protein interactions in the archaeal transcriptional machinery: binding studies of isolated RNA polymerase subunits and transcription factors. J. Biol. Chem. 281, 30581-30592
3. Cramer, P., Bushnell, D.A. and Kornberg, R.D. (2001) Structural basis of transcription: RNA polymerase II at 2.8 Ángstrom resolution. Science 292, 1863-1876
4. Hirata, A., Klein, B.J. and Murakami, K.S. (2008) The X-ray crystal structure of RNA polymerase from Archaea. Nature 451, 851-854
5. Kusser, A.G., Bertero, M.G., Naji, S., Becker, T., Thomm, M., Beckmann, R. and Cramer, P. (2008) Structure of an archaeal RNA polymerase. J. Mol. Biol. 376, 303-307
6. Hausner, W. and Thomm, M. (1995) The translation product of the presumptive Thermococcus celer TATA-binding protein sequence is a transcription factor related in structure and function to Methanococcus transcription factor B. J. Biol. Chem. 270, 17649-17651
7. Bell, S.D. and Jackson, S.P. (2001) Mechanism and regulation of transcription in archaea. Curr. Opin. Microbiol. 4, 208-213
8. Geiduschek, E.P. and Ouhammouch, M. (2005) Archaeal transcription and its regulators. Mol. Microbiol. 56, 1397-1407
9. Meinhart, A., Blobel, J. and Cramer, P. (2003) An extended winged helix domain in general transcription factor E/IIEa. J. Biol. Chem. 278, 48267-48274
10. Werner, F. and Weinzierl, R.O.J. (2005) Direct modulation of RNA polymerase core functions by basal transcription factors. Mol. Cell. Biol. 25, 8344-8355
11. Naji, S., Grünberg, S. and Thomm, M. (2007) The RPB7 orthologue E' is required for transcriptional activity of a reconstituted archaeal core enzyme at low temperatures and stimulates open complex formation. J. Biol. Chem. 282, 11047-11057
12. Grünberg, S., Bartlett, M.S., Naji, S. and Thomm, M. (2007) Transcription factor E is a part of transcription elongation complexes. J. Biol. Chem. 282, 35482-35490
13. Werner, F. and Weinzierl, R.O.J. (2002) A recombinant RNA polymerase II-like enzyme capable of promoter-specific transcription. Mol. Cell 10, 635-646
14. Naji, S., Bertero, M.G., Spitalny, P., Cramer, P. and Thomm, M. (2008) Structure-function analysis of the RNA polymerase cleft loops elucidates initial transcription, DNA unwinding, and RNA displacement. Nucleic Acids Res. 36, 676-687
15. Nottebaum, S., Tan, L., Trzaska, D., Carney, H.C. and Weinzierl, R.O.J. (2008) The RNA polymerase factory: a robotic in vitro assembly platform for high-throughput production of recombinant protein complexes. Nucleic Acids Res. 36, 245-252
16. Wettach, J., Gohl, H.P., Tschochner, H. and Thomm, M. (1995) Functional interaction of yeast and human TATA-binding proteins with an archaeal RNA polymerase and promoter. Proc. Natl. Acad. Sci. U.S.A. 92, 472-476
17. Reich, C., Zeller, M., Milkereit, P., Hausner, W., Cramer, P., Tschochner, H. and Thomm, M. (2009) The archaeal RNA polymerase subunit P and the eukaryotic polymerase subunit Rpb12 are interchangeable in vivo and in vitro. Mol. Microbiol., in the press
18. Schnabel, R., Thomm, M., Gerardy-Schahn, R., Zillig, W., Stetter, K.O. and Huet, J. (1983) Structural homology between different archaebacterial DNA-dependent RNA polymerases analyzed by immunological comparison of their components. EMBO J. 2, 751-755
19. Hethke, C., Geerling, A.C.M., Hausner, W., de Vos, W. and Thomm, M. (1996) A cell-free transcription system for the hyperthermophilic archaeon Pyrococcus furiosus. Nucleic Acids Res. 24, 2369-2376
20. Thomm, M. (2007) Transcription: mechanism and regulation. In Archaea Molecular and Cellular Biology (Cavicchioli, R., ed.), pp. 139-157, ASM, Press, Washington DC
21. Todone, F., Brick, P., Werner, F., Weinzierl, R.O.J. and Onesti, S. (2001) Structure of an archaeal homolog of the eukaryotic RNA polymerase II RPB4/RPB7 complex. Mol. Cell 8, 1137-1143
22. Ouhammouch, M., Werner, F., Weinzierl, R.O.J. and Geiduschek, E.P. (2004) A fully recombinant system for activator-dependent archaeal transcription. J. Biol. Chem. 279, 51719-51721
23. Zawel, L., Kumar, K.P. and Reinberg, D. (1995) Recycling of the general transcription factors during RNA polymerase II transcription. Genes Dev. 9, 1479-1490