Simultaneous quantification of acylated and desacylated ghrelin in biological fluids

Biomedical Chromatography

Volumn 22, Issue 12, 2008, Pages 1354-1359

  Aydin, Suleyman ^a   Karates, Fikret ^b   Geckil, Hikmet ^c  

^a FIRAT UNIVERSITY   (Turkey)

^b FIRAT UNIVERSITY   (Turkey)

^c INONU UNIVERSITY   (Turkey)

Author keywords

Acylated ghrelin; Desacylated ghrelin; Ghrelin; Lipopeptides; N octanoylation

Indexed keywords

ACYLATION; ASSAYS; BODY FLUIDS; COST EFFECTIVENESS;

ACYLATED GHRELIN; BIOLOGICAL FLUIDS; BIOLOGICAL SAMPLES; DESACYLATED GHRELIN; GHRELIN; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; LIPOPEPTIDES; N-OCTANOYLATION;

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

GHRELIN;

ACYLATION AND DEACYLATION; ARTICLE; BODY FLUID; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ELUTION; FEMALE; HORMONE DETERMINATION; HUMAN; MALE; QUANTITATIVE ANALYSIS; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; BLOOD; BREAST MILK; CHEMISTRY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; METHODOLOGY; RADIOIMMUNOASSAY; REPRODUCIBILITY; SALIVA; URINE;

BODY FLUIDS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; FEMALE; GHRELIN; HUMANS; MALE; MILK, HUMAN; RADIOIMMUNOASSAY; REPRODUCIBILITY OF RESULTS; SALIVA;

EID: 59649118194     PISSN: 02693879     EISSN: 10990801     Source Type: Journal    
DOI: 10.1002/bmc.1065     Document Type: Article

References (23)

1. Ariyasu H, Takaya K, Hosoda H, Iwakura H, Ebihara K, Mori K, Ogawa Y, Hosoda K, Akamizu T, Kojima M, Kangawa K and Nakao K. Delayed short-term secretory regulation of ghrelin in obese animals: evidenced by a specific RIA for the active form of ghrelin. Endocrinology 2002; 143: 3341.
2. Aydin S. Discovery of ghrelin hormone, its researches and its clinical applications. Turkish Journal of Biochemistry 2007a; 32: 76.
3. Aydin S. A comparison of ghrelin, glucose, alpha-amylase and protein levels in saliva from diabetics. Journal of Biochemistry and Molecular Biology 2007b; 40(1): 29.
4. Aydin S, Halifeoglu I, Ozercan IH, Erman F, Kilic N, Aydin S, Ilhan N, Ilhan N, Ozkan Y, Akpolat N, Sert L and Caylak E. A comparison of leptin and ghrelin levels in plasma and saliva of young healthy subjects. Peptides 2005; 26: 647.
5. Aydin S, Aydin S, Ozkan Y and Kumru S. Ghrelin is present in human colostrum, transitional and mature milk. Peptides 2006a; 27: 878.
6. Aydin S, Geckil H, Zengin F, Ozercan HI, Karatas F, Aydin S, Turgut-Balik D, Ozkan Y, Dagli F and Celik V. Ghrelin in plants: what is the function of an appetite hormone in plants? Peptides 2006b; 27: 1597.
7. Aydin S, Geckil H, Karatas F, Donder E, Kumru S, Kavak E, Colak R, Ozkan Y and Sahin I. Milk and blood ghrelin level in diabetics. Nutrition 2007; 23: 807.
8. Balasubramaniam A, Wood S, Joshi R, Su C, Friend LA, Sheriff S and James JH. Ghrelin stimulates food intake and growth hormone release in rats with thermal injury: synthesis of ghrelin. Peptides 2006; 27: 1624.
9. Bedendi I, Alloatti G, Marcantoni A, Malan D, Catapano F, Ghé C, Deghenghi R, Ghigo E and Muccioli G. Cardiac effects of ghrelin and its endogenous derivatives des-octanoyl ghrelin and des-Gln14-ghrelin. European Journal of Pharmacology 2003; 476: 87.
10. Dawes P and Dawes E. SGE Choromatography Products Catalog, 2000; 182.
11. Groschl M, Uhr M and Kraus T. Evaluation of the comparability of commercial ghrelin assays. Clinical Chemistry 2004; 50: 457.
12. Groschl M, Topf HG, Bohlender J, Zenk J, Klussmann S, Dötsch J, Rascher W and Rauh M. Identification of ghrelin in human saliva: production by the salivary glands and potential role in proliferation of oral keratinocytes. Clinical Chemistry 2005; 51: 997.
13. Hosoda H, Doi K, Nagaya N, Okumura H, Nakagawa E, Enomoto M, Ono F and Kangawa K. Optimum collection and storage conditions for ghrelin measurements: octanoyl modification of ghrelin is rapidly hydrolyzed to desacyl ghrelin in blood samples. Clinical Chemistry 2004; 50: 1077.
14. Ilcol YO and Hizli B. Active and total ghrelin concentrations increase in breast milk during lactation. Acta Paediatrica 2007; 96(11): 1632.
15. Ishimaru M, Yoshizawa-Kumagaye K, Kubo S, Kitani T, Chino N, Kangawa K and Kimura T. Stability of the O-octanoyl group of rat ghrelin during chemical synthesis: counter-ion-dependent alteration of an ester bond breakage. Letters in Peptide Science 2003; 10: 41.
16. Kierson JA, Dimatteo DM, Locke RG, Mackley AB and Spear ML. Ghrelin and cholecystokinin in term and preterm human breast milk. Acta Paediatrica 2006; 95(8): 991.
17. Kitamura S, Yokota I, Hosoda H, Kotani Y, Matsuda J, Naito E, Ito M, Kangawa K and Kuroda Y. Ghrelin concentration in cord and neonatal blood: relation to fetal growth and energy balance. Journal of Clinical Endocrinology & Metabolism 2003; 88: 5473.
18. Kojima M and Kangawa K. Ghrelin: structure and function. Physiological Reviews 2005; 85: 495.
19. Kojima M, Hosoda H, Date Y, Nakazato M, Matsuo H and Kangawa K. Ghrelin is a growth hormone-releasing acylated peptide from stomach. Nature 1999; 402: 656.
20. Ozkaya O, Buyan N, Bideci A, Gonen S, Ortac E, Fidan K, Cinaz P and Soylemezoglu O. Osteoprotegerin and RANKL serum levels and their relationship with serum ghrelin in children with chronic renal failure and on dialysis. Nephron Clinical Practice 2007; 105(4): c153.
21. Poykko SM, Kellokoski E, Horkko S, Kauma H, Kesaniemi YA and Ukkola O. Low plasma ghrelin is associated with insulin resistance, hypertension, and the prevalence of type 2 diabetes. Diabetes 2003; 52: 2546.
22. Rauh M, Groschl M and Rascher W. Simultaneous quantification of ghrelin and desacyl-ghrelin by liquid chromatography-tandem mass spectrometry in plasma, serum, and cell supernatants. Clinical Chemistry 2007; 53: 902.
23. Yoshimoto A, Mori K, Sugawara A, Mukoyama M, Yahata K, Suganami T, Takaya K, Hosoda H, Kojima M, Kangawa K and Nakao K. Plasma ghrelin and desacyl ghrelin concentrations in renal failure. Journal of the American Society of Nephrology 2002; 13: 2748.