Development of an enzyme-linked immunosorbent assay-based test with a cocktail of nucleocapsid and spike proteins for detection of severe acute respiratory syndrome-associated coronavirus-specific antibody

Clinical and Vaccine Immunology

Volumn 16, Issue 2, 2009, Pages 241-245

  Giménez, Luis G ^a   Rojas, Jose ^a   Rojas, Almudena ^a   Mendoza, Joaquín ^a   Camacho, Ana G ^a  

^a VIRCELL S L   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

IMMUNOGLOBULIN G ANTIBODY; IMMUNOGLOBULIN M ANTIBODY; NUCLEOCAPSID PROTEIN; POLYCLONAL ANTIBODY; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; VIRUS ANTIBODY; VIRUS SPIKE PROTEIN; VIRUS SPIKE PROTEIN[251-682];

ANTIBODY DETECTION; ANTIGEN RECOGNITION; ARTICLE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; IMMUNIZATION; IMMUNOREACTIVITY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SARS CORONAVIRUS; SENSITIVITY AND SPECIFICITY; SERUM;

ANTIBODIES, VIRAL; ANTIGENS, VIRAL; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M; MEMBRANE GLYCOPROTEINS; NUCLEOCAPSID PROTEINS; RECOMBINANT PROTEINS; SARS VIRUS; SENSITIVITY AND SPECIFICITY; SEVERE ACUTE RESPIRATORY SYNDROME; VIRAL ENVELOPE PROTEINS;

EID: 59649103401     PISSN: 15566811     EISSN: 1556679X     Source Type: Journal    
DOI: 10.1128/CVI.00252-08     Document Type: Article

References (27)

1. Brinkmann, U., R. E. Mattes, and P. Buckel. 1989. High-level expression of recombinant genes in Escherichia coli is dependent on the availability of the dna Y gene product. Gene 85:109-114.
2. Callebaut, P., L. Enjuanes, and M. Pensaert. 1996. An adenovirus recombinant expressing the spike glycoprotein of porcine respiratory coronavirus is immunogenic in swine. J. Gen. Virol. 77:309-313.
3. Drosten, C., S. Gunther, W. Preiser, S. van der Werf, H. R. Brodt, S. Becker, H. Rabenau, M. Panning, L. Kolesnikova, R. A. Fouchier, A. Berger, A. M. Burguiere, J. Cinatl, M. Eickmann, N. Escriou, K. Grywna, S. Kramme, J. C. Manuguerra, S. Muller, V. Rickerts, M. Sturmer, S. Vieth, H. D. Klenk, A. D. Osterhaus, H. Schmitz, and H. W. Doerr. 2003. Identification of a novel coronavirus in patients with severe acute respiratory syndrome. N. Engl. J. Med. 348:1967-1976.
4. Freifelder, D. 1983. Physical biochemistry: applications to biochemistry and molecular biology, p.494-536. W. H. Freeman and Company, San Francisco, CA.
5. Gill, S. C., and P. H. von Hippel. 1989. Calculation of protein extinction coefficients from amino acid sequence data. Anal. Biochem. 182:319-326.
6. Gomez, N., C. Carrillo, J. Salinas, F. Parra, M. V. Borca, and J. M. Escribano. 1998. Expression of immunogenic glycoprotein S polypeptides from transmissible gastroenteritis coronavirus in transgenic plants. Virology 249: 352-358.
7. Han, D. P., H. G. Kim, Y. B. Kim, L. L. Poon, and M. W. Cho. 2004. Development of a safe neutralization assay for SARS-CoV and characterization of S-glycoprotein. Virology 326:140-149.
8. Haynes, L. M., C. Miao, J. L. Harcourt, J. M. Montgomery, M. Q. Le, S. A. Dryga, K. I. Kamrud, B. Rivers, G. J. Babcock, J. B. Oliver, J. A. Comer, M. Reynolds, T. M. Uyeki, D. Bausch, T. Ksiazek, W. Thomas, H. Alterson, J. Smith, D. M. Ambrosino, and L. J. Anderson. 2007. Recombinant protein-based assays for detection of antibodies to severe acute respiratory syndrome coronavirus spike and nucleocapsid proteins. Clin. Vaccine Immunol. 14: 331-333.
9. Holmes, K. V. 2001. Coronaviruses, p. 1187-1203. In B. N. Fields, D. M. Knipe, and P. M. Howley (ed.), Fields virology, 4th ed. Lippincott Williams & Wilkins, Philadelphia, PA.
10. Homberger, F. R. 1994. Nucleotide sequence comparison of the membrane protein genes of three enterotopic strains of mouse hepatitis virus. Virus Res. 31:49-56.
11. Jackwood, M. W., and D. A. Hilt. 1995. Production and immunogenicity of multiple antigenic peptide (MAP) constructs derived from the S1 glycoprotein of infectious bronchitis virus (IBV). Adv. Exp. Med. Biol. 380:213-219.
12. Krokhin, O., Y. Li, A. Andonov, H. Feldmann, R. Flick, S. Jones, U. Stroeher, N. Bastien, K. V. Dasuri, K. Cheng, J. N. Simonsen, H. Perreault, J. Wilkins, W. Ens, F. Plummer, and K. G. Standing. 2003. Mass spectrometric characterization of proteins from SARS virus: a preliminary report. Mol. Cell. Proteomics 2:346-356.
13. Li, S., L. Lin, H. Wang, J. Yin, Y. Ren, Z. Zhao, J. Wen, C. Zhou, X. Zhang, X. Li, J. Wang, Z. Zhou, J. Liu, J. Shao, T. Lei, J. Fang, N. Xu, and S. Liu. 2003. The epitope study on the SARS-CoV nucleocapsid protein. Genomics Proteomics Bioinformatics 1:198-206.
14. Lu, L., I. Manopo, B. P. Leung, H. H. Chng, A. E. Ling, L. L. Chee, E. E. Ooi, S. W. Chan, and J. Kwang. 2004. Immunological characterization of the spike protein of the severe acute respiratory syndrome coronavirus. J. Clin. Microbiol. 42:1570-1576.
15. Mahony, J. B., and S. Richardson. 2005. Molecular diagnosis of severe acute respiratory syndrome: the state of the art. J. Mol. Diagn. 7:551-559.
16. McIntosh, K. 1996. Coronaviruses, p. 1095-1103. In B. N. Fields, D. M. Knipe, and P. M. Howley (ed.), Fields virology, 3rd ed., vol. 1. Lippincott-Raven, Philadelphia, PA.
17. Méchin, M. C., M. Der Vartanian, and C. Martín. 1996. The major subunit CIpG of Escherichia coli CS31A fibrillae as an expression vector for different combinations of two TGEV coronavirus epitopes. Gene 179:211-218.
18. Ndifuna, A., A. K. Waters, M. Zhou, and E. W. Collison. 1998. Recombinant nucleocapsid protein is potentially an inexpensive, effective serodiagnostic reagent for infectious bronchitis virus. J. Virol. Methods 70:37-44.
19. Rice, P., I. Longden, and A. Bleasby. 2000. EMBOSS: the European Molecular Biology Open Software Suite. Trends Genet. 16:276-277.
20. Rota, P. A., M. S. Oberste, S. S. Monroe, W. A. Nix, R. Campagnoli, J. P. Icenogle, S. Penaranda, B. Bankamp, K. Maher, M. H. Chen, S. Tong, A. Tamin, L. Lowe, M. Frace, J. L. DeRisi, Q. Chen, D. Wang, D. D. Erdman, T. C. Peret, C. Burns, T. G. Ksiazek, P. E. Rollin, A. Sanchez, S. Liffick, B. Holloway, J. Limor, K. McCaustland, M. Olsen-Rasmussen, R. Fouchier, S. Gunther, A. D. Osterhaus, C. Drosten, M. A. Pallansch, L. J. Anderson, and W. J. Bellini. 2003. Characterization of a novel coronavirus associated with severe acute respiratory syndrome. Science 300:1394-1399.
21. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
22. Studier, F. W. 1990. Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 185:60-89.
23. Vlasova, A. N., X. Zhang, M. Hasoksuz, H. S. Nagesha, L. M. Haynes, Y. Fang, S. Lu, and L. J. Saif. 2007. Two-way antigenic cross-reactivity between severe acute respiratory syndrome coronavirus (SARS-CoV) and group 1 animal CoV is mediated through an antigenic site in the N-terminal region of the SARS-CoV nucleoprotein. J. Virol. 81:13365-13377.
24. Woo, P. C., S. K. Lau, B. H. Wong, H. W. Tsoi, A. M. Fung, R. Y. Kao, K. H. Chan, J. S. Peiris, and K. Y. Yuen. 2005. Differential sensitivities of severe acute respiratory syndrome (SARS) coronavirus spike polypeptide enzyme-linked immunosorbent assay (ELISA) and SARS coronavirus nucleocapsid protein ELISA for serodiagnosis of SARS coronavirus pneumonia. J. Clin. Microbiol. 43:3054-3058.
25. Yu, F., M. Q. Le, S. Inoue, F. Hasebe, C. Parquet Mdel, S. Morikawa, and K Morita. 2007. Recombinant truncated nucleocapsid protein as antigen in a novel immunoglobulin M capture enzyme-linked immunosorbent assay for diagnosis of severe acute respiratory syndrome coronavirus infection. Clin. Vaccine Immunol. 14:146-149.
26. Zhao, J., Q. Huang, W. Wang, Y. Zhang, P. Lv, and X.-M. Gao. 2007. Identification and characterization of dominant helper T-cell epitopes in the nucleocapsid protein of severe acute respiratory syndrome coronavirus. J. Virol. 81:6079-6088.
27. Zhou, T., H. Wang, D. Luo, T. Rowe, Z. Wang, R. J. Hogan, S. Qiu, R. J. Bunzel, G. Huang, V. Mishra, T. G. Voss, R. Kimberly, and M. Luo. 2004. An exposed domain in the severe acute respiratory syndrome coronavirus spike protein induces neutralizing antibodies. J. Virol. 78:7217-7226.