Assessment of biochemical methods to detect enzymatic depolymerization of polysaccharides

Carbohydrate Polymers

Volumn 76, Issue 2, 2009, Pages 279-284

  Rigouin, C ^a,b   Ladrat, C Delbarre ^a   Sinquin, C ^a   Colliec Jouault, S ^a   Dion, M ^b  

^a IFREMER   (France)

^b UNIVERSITÉ DE NANTES   (France)

Author keywords

Carbohydrate; Depolymerization; Detection; Enzyme

Indexed keywords

ASSAYS; BIOPOLYMERS; CARBOHYDRATES; CHROMATOGRAPHIC ANALYSIS; ELECTROPHORESIS; ENZYMES; GELATION; ORGANIC COMPOUNDS; POLYSACCHARIDES; SUGAR (SUCROSE); SUGARS;

AGAROSE GEL ELECTROPHORESIS; BIOCHEMICAL METHODS; CARBOHYDRATE; DEPOLYMERIZATION; DETECTION; DOUBLE BONDS; ENZYMATIC DEPOLYMERIZATION; ENZYME; HIGH PERFORMANCE SIZE EXCLUSION CHROMATOGRAPHIES; HIGH THROUGHPUTS; MOLECULAR WEIGHT DETERMINATIONS; POLYSACCHARIDE DEGRADATIONS; QUALITATIVE ASSAYS; REDUCING SUGARS;

SIZE EXCLUSION CHROMATOGRAPHY;

EID: 59449102139     PISSN: 01448617     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.carbpol.2008.10.022     Document Type: Article

References (29)

1. 2+-binding proteins, calsequestrin, calmodulin, troponin C, and S-100, with the cationic carbocyanine dye "Stains-all". Journal of Biological Chemistry 258 18 (1983) 11267-11273
2. Colliec Jouault S., Chevolot L., Helley D., Ratiskol J., Bros A., Sinquin C., et al. Characterization, chemical modifications and in vitro anticoagulant properties of an exopolysaccharide produced by Alteromonas infernus. Biochimica et Biophysica Acta 1528 2-3 (2001) 141-151
3. Colliec-Jouault S., Zanchetta P., Helley D., Ratiskol J., Sinquin C., Fischer A.M., et al. Les polysaccharides microbiens d'origine marine et leur potentiel en therapeutique humaine. Pathologie Biologie 52 3 (2004) 127-130
4. ®, a useful substrate to select chitinolytic microorganisms and to evaluate chitinases. Journal of Microbiological Methods 56 2 (2004) 213-219
5. Guezennec J., Pignet P., Lijour Y., Gentric E., Ratiskol J., and Colliec-Jouault S. Sulfation and depolymerization of a bacterial exopolysaccharide of hydrothermal origin. Carbohydrate Polymers 37 1 (1998) 19-24
6. Jing W., Michael Haller F., Almond A., and DeAngelis P.L. Defined megadalton hyaluronan polymer standards. Analytical Biochemistry 355 2 (2006) 183-188
7. Kühn A.V., Ozegowski J.-H., Peschel G., and Neubert R.H.H. Complementary exploration of the action pattern of hyaluronate lyase from Streptococcus agalactiae using capillary electrophoresis, gel-permeation chromatography and viscosimetric measurements. Carbohydrate Research 339 15 (2004) 2541-2547
8. Lee H.G., and Cowman M.K. An agarose-gel electrophoretic method for analysis of hyaluronan molecular-weight distribution. Analytical Biochemistry 219 2 (1994) 278-287
9. Meeuwsen P.J.A., Vincken J.-P., Beldman G., and Voragen A.G.J. A universal assay for screening expression libraries for carbohydrases. Journal of Bioscience and Bioengineering 89 1 (2000) 107-109
10. Miller G.L. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry 31 3 (1959) 426-428
11. Myklestad S.M., Skånøy E., and Hestmann S. A sensitive and rapid method for analysis of dissolved mono- and polysaccharides in seawater. Marine Chemistry 56 3-4 (1997) 279-286
12. Nardella A., Chaubet F., Boisson-Vidal C., Blondin C., Durand P., and Jozefonvicz J. Anticoagulant low molecular weight fucans produced by radical process and ion exchange chromatography of high molecular weight fucans extracted from the brown seaweed Ascophyllum nodosum. Carbohydrate Research 289 (1996) 201-208
13. Nedjma M., Hoffmann N., and Belarbi A. Selective and sensitive detection of pectin lyase activity using a colorimetric test: Application to the screening of microorganisms possessing pectin lyase activity. Analytical Biochemistry 291 2 (2001) 290-296
14. Nelson, N. (1977). Citation classics - Photometric adaptation of Somogyi method for determination of glucose. Current Contents (3), 13.
15. Ofman D., Slim G.C., Watt D.K., and Yorke S.C. Free radical induced oxidative depolymerisation of chondroitin sulphate and dermatan sulphate. Carbohydrate Polymers 33 1 (1997) 47-56
16. Parisot J., Ghochikyan A., Langlois V., Sakanyan V., and Rabiller C. Exopolygalacturonate lyase from Thermotoga maritima: Cloning, characterization and organic synthesis application. Carbohydrate Research 337 16 (2002) 1427-1433
17. Park J.T., and Johnson M.J. A submicrodetermination of glucose. Journal of Biological Chemistry 181 1 (1949) 149-151
18. Pettersson B., and Eriksson K.-E.L. A standardized spectrophotometric assay of endoglycanase activities using dyed, amorphous polysaccharides. Analytical Biochemistry 285 2 (2000) 220-224
19. Reissig J.L., Storminger J.L., and Leloir L.F. A modified colorimetric method for the estimation of N-acetylamino sugars. Journal of Biological Chemistry 217 2 (1955) 959-966
20. Roubroeks J.P., Skjak-Braek G., Ryan L., and Christensen B.E. Molecular weight dependency on the production of the TNF stimulated by fractions of rye (1→3),(1→4)-β-d-glucan. Scandinavian Journal of Immunology 52 6 (2000) 584-587
21. Ruijssenaars H.J., and Hartmans S. Plate screening methods for the detection of polysaccharase-producing microorganisms. Applied Microbiology and Biotechnology 55 (2001) 143-149
22. Samama M.M., and Desnoyers P.C. Low-molecular-weight heparins: Biochemistry and pharmacology. In: Sasahara A.A., and Loscalzo J. (Eds). New therapeutic agents in thrombosis and thrombolysis (1997), Marcel Dekker, Inc., New York 87-101
23. Shively J.E., and Conrad H.E. Formation of anhydrosugars in the chemical depolymerization of heparin. Biochemistry 15 18 (1976) 3932-3942
24. Sutherland I.W. Polysaccharide lyases. FEMS Microbiology Reviews 16 4 (1995) 323-347
25. Ten L.N., Im W.-T., Kim M.-K., Kang M.S., and Lee S.-T. Development of a plate technique for screening of polysaccharide-degrading microorganisms by using a mixture of insoluble chromogenic substrates. Journal of Microbiological Methods 56 3 (2004) 375-382
26. Volpi N., and Maccari F. Electrophoretic approaches to the analysis of complex polysaccharides. Journal of Chromatography B 834 1-2 (2006) 1-13
27. Weitz J.I., and Hirsh J. Overview of new developments in heparins, thrombin inhibitors, and other new and novel agents. In: Sasahara A.A., and Loscalzo J. (Eds). New therapeutic agents in thrombosis and thrombolysis (1997), Marcel Dekker, Inc., New York (pp. 77-86)
28. Wood P.J., Erfle J.D., and Teather R.M. Use of complex formation between Congo Red and polysaccharides in detection and assay of polysaccharides hydrolases. Methods in Enzymology 160 (1988) 59-74
29. Zanchetta P., Lagarde N., and Guezennec J. A new bone-healing material: A hyaluronic acid-like bacterial exopolysaccharide. Calcified Tissue International 72 1 (2003) 74-79