Isolation of photoreceptors in the cultured full-thickness fetal rat retina

Investigative Ophthalmology and Visual Science

Volumn 50, Issue 2, 2009, Pages 826-835

  Ghosh, Fredrik ^a   Arnér, Karin ^a   Engelsberg, Karl ^a  

^a LUND UNIVERSITY HOSPITAL   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

CALBINDIN; PARVALBUMIN; RHODOPSIN; TRANSDUCIN; TUBULIN; VIMENTIN; BIOLOGICAL MARKER;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL CULTURE; CELL ISOLATION; CELL LABELING; CELL TYPE; CONTROLLED STUDY; EMBRYO DEVELOPMENT; EXPLANT; MICROSCOPY; MUELLER CELL; NERVE CELL; NONHUMAN; PHOTORECEPTOR CELL; PRESYNAPTIC MEMBRANE; PRIORITY JOURNAL; RAT; RETINA AMACRINE CELL; RETINA CELL; RETINA SURGERY; ANIMAL; CELL DIFFERENTIATION; CELL SEPARATION; CELL SURVIVAL; CYTOLOGY; FEMALE; FLUORESCENT ANTIBODY TECHNIQUE; GLIA; MALE; METABOLISM; ORGAN CULTURE TECHNIQUE; PHYSIOLOGY; PREGNANCY; PRENATAL DEVELOPMENT; RETINA; RETINA BIPOLAR GANGLION CELL; RETINA GANGLION CELL; SPRAGUE DAWLEY RAT; SYNAPSE VESICLE;

ANIMALS; BIOLOGICAL MARKERS; CELL DIFFERENTIATION; CELL SEPARATION; CELL SURVIVAL; FEMALE; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; MALE; NEUROGLIA; ORGAN CULTURE TECHNIQUES; PHOTORECEPTOR CELLS, VERTEBRATE; PREGNANCY; RATS; RATS, SPRAGUE-DAWLEY; RETINA; RETINAL BIPOLAR CELLS; RETINAL GANGLION CELLS; SYNAPTIC VESICLES;

EID: 59449089956     PISSN: 01460404     EISSN: 15525783     Source Type: Journal    
DOI: 10.1167/iovs.08-2389     Document Type: Article

References (33)

1. Turner JE, Blair JR. Newborn rat retinal cells transplanted into a retinal lesion site in adult host eyes. Brain Res. 1986;391:91-104.
2. Seiler MJ, Aramant RB. Intact sheets of fetal retina transplanted to restore damaged rat retinas. Invest Ophthalmol Vis Sci. 1998;39: 2121-2131.
3. Ghosh F, Johansson K, Ehinger B. Long-term full-thickness embryonic rabbit retinal transplants. Invest Ophthalmol Vis Sci. 1999; 40:133-140.
4. Ghosh F, Engelsberg K, English RV, Petters RM. Long-term neuroretinal full-thickness transplants in severely degenerated rhodopsin transgenic pigs. Graefe's Arch Clin Exp Ophthalmol. 2007;245: 835-846.
5. Ghosh F, Bruun A, Ehinger B. Graft-host connections in long-term full thickness embryonic rabbit retinal transplants. Invest Ophthalmol Vis Sci. 1999;40:126-132.
6. del Cerro M, Yeh HH, Marrero-Rodriguez A, Lazar E, del Cerro C. Intraocular transplantation of cell layers derived from neonatal rat retina. Brain Res. 1990;535:25-32.
7. Silverman MS, Hughes SE. Transplantation of photoreceptors to light-damaged retina. Invest Ophthalmol Vis Sci. 1989;30:1684-1690.
8. Tezel TH, Kaplan HJ. Harvest and storage of adult human photoreceptor cells: the vibratome compared to the excimer laser. Curr Eye Res. 1998;17:748-756.
9. Ghosh F, Juliusson B, Arnér K, Ehinger B. Partial and full-thickness neuroretinal transplants. Exp Eye Res. 1999;68:67-74.
10. Gouras P, Du J, Gelanze M, Kwun R, Kjeldbye H, Lopez R. Transplantation of photoreceptors labeled with tritiated thymidine into RCS rats. Invest Ophthalmol Vis Sci. 1991;32:1704-1707.
11. Schuschereba ST, Silverman MS. Retinal cell and photoreceptor transplantation between adult New Zealand red rabbit retinas. Exp Neurol. 1992;115:95-99.
12. Emgård M, Blomgren K, Brundin P. Characterisation of cell damage and death in embryonic mesencephalic tissue: a study on ultrastructure, vital stains and protease activity. Neuroscience. 2002; 115:1177-1187.
13. Cepko CL, Austin CP, Yang X, Alexiades M, Ezzeddine D. Cell fate determination in the vertebrate retina. Proc Natl Acad Sci U S A. 1996;93:589-595.
14. Rapaport DH, Wong LL, Wood ED, Yasumura D, LaVail MM. Timing and topography of cell genesis in the rat retina. J Comp Neurol. 2004;474:304-324.
15. Rickman DW. Parvalbumin immunoreactivity is enhanced by brain-derived neurotrophic factor in organotypic cultures of rat retina. Neurobiology. 1999;41:376-384.
16. Cusato K, Stagg SB, Reese BE. Two phases of increased cell death in the inner retina following early elimination of the ganglion cell population. J Comp Neurol. 2001;439:440-449.
17. Osakada F, Ikeda H, Mandai M, et al. Toward the generation of rod and cone photoreceptors from mouse, monkey and human embryonic stem cells. Nat Biotechnol. 2008;26:187-188.
18. Rothermel A, Layer PG. GDNF regulates chicken rod photoreceptor development and survival in reaggregated histotypic retinal spheres. Invest Ophthalmol Vis Sci. 2003;44:2221-2228.
19. LaVail MM, Hild W. Histotypic organization of the rat retina in vitro. Z Zellforsch Mikrosk Anat. 1971;114:557-579.
20. Johansson K, Bruun A, Grasbon T, Ehinger B. Growth of postnatal rat retina in vitro: development of neurotransmitter systems. J Chem Neuroanat. 2000;19:117-128.
21. Sparrow JR, Hicks D, Barnstable CJ. Cell commitment and differentiation in explants of embryonic rat neural retina: comparison with the developmental potential of dissociated retina. Brain Res Dev Brain Res. 1990;51:69-84.
22. Dodge J, Fulton AB, Parker C, Hansen RM, Williams TP. Rhodopsin in immature rod outer segments. Invest Ophthalmol Vis Sci. 1996; 37:1951-1956.
23. Kuwabara T. Development of the optic nerve of the rat. Invest Ophthalmol Vis Sci. 1975;14:732-745.
24. Shaw G, Weber K. The structure and development of the rat retina: an immunofluorescence microscopical study using antibodies specific for intermediate filament proteins. Eur J Cell Biol. 1983;30:219-232.
25. Liljekvist-Larsson I, Törngren M, Abrahamson M, Johansson K. Growth of the postnatal rat retina in vitro: quantitative RT-PCR analyses of mRNA expression for photoreceptor proteins. Mol Vis. 2003;9:657-664.
26. Engelsberg K, Ehinger B, Wasselius J, Johansson K. Apoptotic cell death and microglial cell responses in cultured rat retina. Graefe's Arch Clin Exp Ophtahalmol. 2004;242:229-239.
27. Yamasaki EN, Andrade da Costa BL, Barbosa VD, Hokoç JN. Retinal ganglion cell depletion alters the phenotypic expression of GABA and GAD in the rat retina. Eur J Neurosci. 1997;9:1885-1890.
28. Belliveau MJ, Cepko CL. Extrinsic and intrinsic factors control the genesis of amacrine and cone cells in the rat retina. Development. 1999;126:555-566.
29. Pinzón-Duarte G, Kohler K, Arango-González B, Guenther E. Cell differentiation, synaptogenesis, and influence of the retinal pigment epithelium in a rat neonatal organotypic retina culture. Vision Res. 2000;40:3455-3465.
30. Johnson PT, Williams RR, Cusato K, Reese BE. Rods and cones project to the inner plexiform layer during development. J Comp Neurol. 1999;414:1-12.
31. Kapfhammer JP, Christ F, Schwab ME. The expression of GAP-43 and synaptophysin in the developing rat retina. Brain Res Dev Brain Res. 1994;48:251-260.
32. Germer A, Kuhnel K, Grosche J, et al. Development of the neonatal rabbit retina in organ culture, 1: comparison with histogenesis in vivo, and the effect of a gliotoxin (alpha-aminoadipic acid). Anat Embryol. 1997;196:67-79.
33. Kljavin IJ, Reh TA. Muller cells are a preferred substrate for in vitro neurite extension by rod photoreceptor cells. J Neurosci. 1991; 11:2985-2994.