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Volumn 451, Issue C, 2009, Pages 63-83

Chapter 5 Sequestration Assays for Mammalian Autophagy

Author keywords

[No Author keywords available]

Indexed keywords

LACTATE DEHYDROGENASE; LACTOSE; RAFFINOSE; SUCROSE; BETA FRUCTOFURANOSIDASE; BETA GALACTOSIDASE; CARBOHYDRATE; DIGITONIN; GALACTOSE; HOMOCYSTEINE METHYLTRANSFERASE; LEUPEPTIN;

EID: 59349105750     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(08)03605-7     Document Type: Review
Times cited : (18)

References (62)
  • 1
    • 0032555641 scopus 로고    scopus 로고
    • Isolation and characterization of rat liver amphisomes: Evidence for fusion of autophagosomes with both early and late endosomes
    • Berg T.O., Fengsrud M., Strømhaug P.E., Berg T., and Seglen P.O. Isolation and characterization of rat liver amphisomes: Evidence for fusion of autophagosomes with both early and late endosomes. J. Biol. Chem. 273 (1998) 21883-21892
    • (1998) J. Biol. Chem. , vol.273 , pp. 21883-21892
    • Berg, T.O.1    Fengsrud, M.2    Strømhaug, P.E.3    Berg, T.4    Seglen, P.O.5
  • 3
    • 0027716683 scopus 로고
    • Tracing intracellular proteolytic pathways: Proteolysis of fatty acid synthase and other cytoplasmic proteins in the yeast Saccharomyces cerevisiae
    • Egner R., Thumm M., Straub M., Simeon A., Schuller H.J., and Wolf D.H. Tracing intracellular proteolytic pathways: Proteolysis of fatty acid synthase and other cytoplasmic proteins in the yeast Saccharomyces cerevisiae. J. Biol. Chem. 268 (1993) 27269-27276
    • (1993) J. Biol. Chem. , vol.268 , pp. 27269-27276
    • Egner, R.1    Thumm, M.2    Straub, M.3    Simeon, A.4    Schuller, H.J.5    Wolf, D.H.6
  • 7
    • 0035117521 scopus 로고    scopus 로고
    • Leupeptin-induced appearance of partial fragment of betaine homocysteine methyltransferase during autophagic maturation in rat hepatocytes
    • Furuya N., Kanazawa T., Fujimura S., Ueno T., Kominami E., and Kadowaki M. Leupeptin-induced appearance of partial fragment of betaine homocysteine methyltransferase during autophagic maturation in rat hepatocytes. J. Biochem. (Tokyo) 129 (2001) 313-320
    • (2001) J. Biochem. (Tokyo) , vol.129 , pp. 313-320
    • Furuya, N.1    Kanazawa, T.2    Fujimura, S.3    Ueno, T.4    Kominami, E.5    Kadowaki, M.6
  • 8
    • 0026559695 scopus 로고
    • Prelysosomal and lysosomal connections between autophagy and endocytosis
    • Gordon P.B., Høyvik H., and Seglen P.O. Prelysosomal and lysosomal connections between autophagy and endocytosis. Biochem. J. 283 (1992) 361-369
    • (1992) Biochem. J. , vol.283 , pp. 361-369
    • Gordon, P.B.1    Høyvik, H.2    Seglen, P.O.3
  • 9
    • 0024299286 scopus 로고
    • Prelysosomal convergence of autophagic and endocytic pathways
    • Gordon P.B., and Seglen P.O. Prelysosomal convergence of autophagic and endocytic pathways. Biochem. Biophys. Res. Commun. 151 (1988) 40-47
    • (1988) Biochem. Biophys. Res. Commun. , vol.151 , pp. 40-47
    • Gordon, P.B.1    Seglen, P.O.2
  • 10
    • 0022896893 scopus 로고
    • Use of electrical methods in the study of hepatocytic autophagy
    • Gordon P.B., and Seglen P.O. Use of electrical methods in the study of hepatocytic autophagy. Biomed. Biochim. Acta 45 (1986) 1635-1645
    • (1986) Biomed. Biochim. Acta , vol.45 , pp. 1635-1645
    • Gordon, P.B.1    Seglen, P.O.2
  • 11
    • 0020334810 scopus 로고
    • 14C]sucrose, introduced into isolated rat hepatocytes by electropermeabilization
    • 14C]sucrose, introduced into isolated rat hepatocytes by electropermeabilization. Exp. Cell Res. 142 (1982) 1-14
    • (1982) Exp. Cell Res. , vol.142 , pp. 1-14
    • Gordon, P.B.1    Seglen, P.O.2
  • 13
    • 0022268071 scopus 로고
    • 14C]sucrose introduced into isolated rat hepatocytes by electrical and non-electrical methods
    • 14C]sucrose introduced into isolated rat hepatocytes by electrical and non-electrical methods. Exp. Cell Res. 160 (1985) 449-458
    • (1985) Exp. Cell Res. , vol.160 , pp. 449-458
    • Gordon, P.B.1    Tolleshaug, H.2    Seglen, P.O.3
  • 14
    • 0025858399 scopus 로고
    • Inhibition of autophagic-lysosomal delivery and autophagic lactolysis by asparagine
    • Høyvik H., Gordon P.B., Berg T.O., Strømhaug P.E., and Seglen P.O. Inhibition of autophagic-lysosomal delivery and autophagic lactolysis by asparagine. J. Cell Biol. 113 (1991) 1305-1312
    • (1991) J. Cell Biol. , vol.113 , pp. 1305-1312
    • Høyvik, H.1    Gordon, P.B.2    Berg, T.O.3    Strømhaug, P.E.4    Seglen, P.O.5
  • 15
    • 0022447864 scopus 로고
    • 14C]lactose, to distinguish between pre-lysosomal and lysosomal steps in the autophagic pathway
    • 14C]lactose, to distinguish between pre-lysosomal and lysosomal steps in the autophagic pathway. Exp. Cell Res. 166 (1986) 1-14
    • (1986) Exp. Cell Res. , vol.166 , pp. 1-14
    • Høyvik, H.1    Gordon, P.B.2    Seglen, P.O.3
  • 16
    • 0008853116 scopus 로고
    • Convergence of autophagic and endocytic pathways at the level of the lysosome
    • Høyvik H., Gordon P.B., and Seglen P.O. Convergence of autophagic and endocytic pathways at the level of the lysosome. Biochem. Soc. Transact. 15 (1987) 964-965
    • (1987) Biochem. Soc. Transact. , vol.15 , pp. 964-965
    • Høyvik, H.1    Gordon, P.B.2    Seglen, P.O.3
  • 20
    • 0025051251 scopus 로고
    • Non-selective autophagy of cytosolic enzymes in isolated rat hepatocytes
    • Kopitz J., Kisen G.Ø., Gordon P.B., Bohley P., and Seglen P.O. Non-selective autophagy of cytosolic enzymes in isolated rat hepatocytes. J. Cell Biol. 111 (1990) 941-953
    • (1990) J. Cell Biol. , vol.111 , pp. 941-953
    • Kopitz, J.1    Kisen, G.Ø.2    Gordon, P.B.3    Bohley, P.4    Seglen, P.O.5
  • 21
    • 0019417043 scopus 로고
    • Inhibition of autophagic vacuole formation and protein degradation by amino acids in isolated hepatocytes
    • Kovács A.L., Grinde B., and Seglen P.O. Inhibition of autophagic vacuole formation and protein degradation by amino acids in isolated hepatocytes. Exp. Cell Res. 133 (1981) 431-436
    • (1981) Exp. Cell Res. , vol.133 , pp. 431-436
    • Kovács, A.L.1    Grinde, B.2    Seglen, P.O.3
  • 22
    • 0020040519 scopus 로고
    • Accumulation of autophagosomes after inhibition of hepatocytic protein degradation by vinblastine, leupeptin or a lysosomotropic amine
    • Kovács A.L., Reith A., and Seglen P.O. Accumulation of autophagosomes after inhibition of hepatocytic protein degradation by vinblastine, leupeptin or a lysosomotropic amine. Exp. Cell Res. 137 (1982) 191-201
    • (1982) Exp. Cell Res. , vol.137 , pp. 191-201
    • Kovács, A.L.1    Reith, A.2    Seglen, P.O.3
  • 23
    • 0023723057 scopus 로고
    • Protein microinjection by protease permeabilization of fibroblasts
    • Lemons R., Forster S., and Thoene J. Protein microinjection by protease permeabilization of fibroblasts. Anal. Biochem. 172 (1988) 219-227
    • (1988) Anal. Biochem. , vol.172 , pp. 219-227
    • Lemons, R.1    Forster, S.2    Thoene, J.3
  • 24
    • 33749052075 scopus 로고    scopus 로고
    • Signalling and autophagy regulation in health, aging and disease
    • Meijer A.J., and Codogno P. Signalling and autophagy regulation in health, aging and disease. Mol. Aspects Med. 27 (2006) 411-425
    • (2006) Mol. Aspects Med. , vol.27 , pp. 411-425
    • Meijer, A.J.1    Codogno, P.2
  • 25
    • 38949146398 scopus 로고    scopus 로고
    • Macroautophagy-dependent, intralysosomal cleavage of a betaine homocysteine methyltransferase fusion protein requires stable multimerization
    • Mercer C.A., Kaliappan A., and Dennis P.B. Macroautophagy-dependent, intralysosomal cleavage of a betaine homocysteine methyltransferase fusion protein requires stable multimerization. Autophagy 4 (2008) 185-194
    • (2008) Autophagy , vol.4 , pp. 185-194
    • Mercer, C.A.1    Kaliappan, A.2    Dennis, P.B.3
  • 26
    • 0035830631 scopus 로고    scopus 로고
    • Effects of inhibitors of the vacuolar proton pump on hepatic heterophagy and autophagy
    • Mousavi S.A., Kjeken R., Berg T.O., Seglen P.O., Berg T., and Brech A. Effects of inhibitors of the vacuolar proton pump on hepatic heterophagy and autophagy. Biochim. Biophys. Acta 1510 (2001) 243-257
    • (2001) Biochim. Biophys. Acta , vol.1510 , pp. 243-257
    • Mousavi, S.A.1    Kjeken, R.2    Berg, T.O.3    Seglen, P.O.4    Berg, T.5    Brech, A.6
  • 28
    • 0014874755 scopus 로고
    • Endocytosis of sucrose and other sugars by cells in culture
    • Nyberg E., and Dingle J.T. Endocytosis of sucrose and other sugars by cells in culture. Exp. Cell Res. 63 (1970) 43-52
    • (1970) Exp. Cell Res. , vol.63 , pp. 43-52
    • Nyberg, E.1    Dingle, J.T.2
  • 29
    • 0020321716 scopus 로고
    • Introduction of macromolecules into cultured mammalian cells by osmotic lysis of pinoytic vesicles
    • Okada C.Y., and Rechsteiner M. Introduction of macromolecules into cultured mammalian cells by osmotic lysis of pinoytic vesicles. Cell 29 (1982) 33-41
    • (1982) Cell , vol.29 , pp. 33-41
    • Okada, C.Y.1    Rechsteiner, M.2
  • 31
    • 14844366592 scopus 로고    scopus 로고
    • Stimulation of hepatocytic AMP-activated protein kinase by okadaic acid and other autophagy-suppressive toxins
    • Samari H.R., Møller M.T.N., Holden L., Asmyhr T., and Seglen P.O. Stimulation of hepatocytic AMP-activated protein kinase by okadaic acid and other autophagy-suppressive toxins. Biochem. J. 386 (2005) 237-244
    • (2005) Biochem. J. , vol.386 , pp. 237-244
    • Samari, H.R.1    Møller, M.T.N.2    Holden, L.3    Asmyhr, T.4    Seglen, P.O.5
  • 33
    • 0032996570 scopus 로고    scopus 로고
    • Parasitophorous vacuoles of Leishmania mexicana acquire macromolecules from the host cell cytosol via two independent routes
    • Schaible U.E., Schlesinger P.H., Steinberg T.H., Mangel W.F., Kobayashi T., and Russell D.G. Parasitophorous vacuoles of Leishmania mexicana acquire macromolecules from the host cell cytosol via two independent routes. J. Cell Sci. 112 (1999) 681-693
    • (1999) J. Cell Sci. , vol.112 , pp. 681-693
    • Schaible, U.E.1    Schlesinger, P.H.2    Steinberg, T.H.3    Mangel, W.F.4    Kobayashi, T.5    Russell, D.G.6
  • 34
    • 0344894871 scopus 로고
    • Isolation of hepatocytes
    • Celis J.E. (Ed), Academic Press, San Diego
    • Seglen P.O. Isolation of hepatocytes. In: Celis J.E. (Ed). Handbook of cell biology (1994), Academic Press, San Diego 96-102
    • (1994) Handbook of cell biology , pp. 96-102
    • Seglen, P.O.1
  • 35
    • 0020973226 scopus 로고
    • Inhibitors of lysosomal function
    • Seglen P.O. Inhibitors of lysosomal function. Methods Enzymol. 96 (1983) 737-764
    • (1983) Methods Enzymol. , vol.96 , pp. 737-764
    • Seglen, P.O.1
  • 36
    • 4243759951 scopus 로고    scopus 로고
    • Isolation of hepatocytes
    • Celis J.E. (Ed), Academic Press, San Diego
    • Seglen P.O. Isolation of hepatocytes. In: Celis J.E. (Ed). Cell biology: a laboratory handbook (1998), Academic Press, San Diego 119-124
    • (1998) Cell biology: a laboratory handbook , pp. 119-124
    • Seglen, P.O.1
  • 37
    • 0001471696 scopus 로고
    • Isolation of hepatocytes by collagenase perfusion
    • Seglen P.O. Isolation of hepatocytes by collagenase perfusion. Methods Toxicol. 1A (1993) 231-243
    • (1993) Methods Toxicol. , vol.1 A , pp. 231-243
    • Seglen, P.O.1
  • 38
    • 0016778074 scopus 로고
    • Protein degradation in isolated rat hepatocytes is inhibited by ammonia
    • Seglen P.O. Protein degradation in isolated rat hepatocytes is inhibited by ammonia. Biochem. Biophys. Res. Commun. 66 (1975) 44-52
    • (1975) Biochem. Biophys. Res. Commun. , vol.66 , pp. 44-52
    • Seglen, P.O.1
  • 39
    • 0017101039 scopus 로고
    • Preparation of isolated rat liver cells
    • Seglen P.O. Preparation of isolated rat liver cells. Methods Cell Biol. 13 (1976) 29-83
    • (1976) Methods Cell Biol. , vol.13 , pp. 29-83
    • Seglen, P.O.1
  • 40
    • 0021271399 scopus 로고
    • Amino acid control of autophagic sequestration and protein degradation in isolated rat hepatocytes
    • Seglen P.O., and Gordon P.B. Amino acid control of autophagic sequestration and protein degradation in isolated rat hepatocytes. J. Cell Biol. 99 (1984) 435-444
    • (1984) J. Cell Biol. , vol.99 , pp. 435-444
    • Seglen, P.O.1    Gordon, P.B.2
  • 41
    • 0019209783 scopus 로고
    • Effects of lysosomotropic monoamines, diamines, amino alcohols, and other amino compounds on protein degradation and protein synthesis in isolated rat hepatocytes
    • Seglen P.O., and Gordon P.B. Effects of lysosomotropic monoamines, diamines, amino alcohols, and other amino compounds on protein degradation and protein synthesis in isolated rat hepatocytes. Mol. Pharmacol. 18 (1980) 468-475
    • (1980) Mol. Pharmacol. , vol.18 , pp. 468-475
    • Seglen, P.O.1    Gordon, P.B.2
  • 42
    • 0005677775 scopus 로고
    • 3-Methyladenine: A specific inhibitor of autophagic/lysosomal protein degradation in isolated rat hepatocytes
    • Seglen P.O., and Gordon P.B. 3-Methyladenine: A specific inhibitor of autophagic/lysosomal protein degradation in isolated rat hepatocytes. Proc. Natl. Acad. Sci. USA 79 (1982) 1889-1892
    • (1982) Proc. Natl. Acad. Sci. USA , vol.79 , pp. 1889-1892
    • Seglen, P.O.1    Gordon, P.B.2
  • 43
    • 0022904943 scopus 로고
    • Radiolabelled sugars as probes of hepatocytic autophagy
    • Seglen P.O., Gordon P.B., and Høyvik H. Radiolabelled sugars as probes of hepatocytic autophagy. Biomed. Biochim. Acta 45 (1986) 1647-1656
    • (1986) Biomed. Biochim. Acta , vol.45 , pp. 1647-1656
    • Seglen, P.O.1    Gordon, P.B.2    Høyvik, H.3
  • 45
    • 0017082272 scopus 로고
    • Ammonia inhibition of protein degradation in isolated rat hepatocytes: Quantitative ultrastructural alterations in the lysosomal system
    • Seglen P.O., and Reith A. Ammonia inhibition of protein degradation in isolated rat hepatocytes: Quantitative ultrastructural alterations in the lysosomal system. Exp. Cell Res. 100 (1976) 276-280
    • (1976) Exp. Cell Res. , vol.100 , pp. 276-280
    • Seglen, P.O.1    Reith, A.2
  • 46
    • 0017667822 scopus 로고
    • Evidence for the autophagy of microinjected proteins in HeLa cells
    • Stacey D.W., and Allfrey V.G. Evidence for the autophagy of microinjected proteins in HeLa cells. J. Cell Biol. 75 (1977) 807-817
    • (1977) J. Cell Biol. , vol.75 , pp. 807-817
    • Stacey, D.W.1    Allfrey, V.G.2
  • 47
    • 0032532323 scopus 로고    scopus 로고
    • Purification and characterization of autophagosomes from rat hepatocytes
    • Strømhaug P.E., Berg T.O., Fengsrud M., and Seglen P.O. Purification and characterization of autophagosomes from rat hepatocytes. Biochem. J. 335 (1998) 217-224
    • (1998) Biochem. J. , vol.335 , pp. 217-224
    • Strømhaug, P.E.1    Berg, T.O.2    Fengsrud, M.3    Seglen, P.O.4
  • 48
    • 0027513693 scopus 로고
    • Evidence for acidity of prelysosomal autophagic/endocytic vacuoles (amphisomes)
    • Strømhaug P.E., and Seglen P.O. Evidence for acidity of prelysosomal autophagic/endocytic vacuoles (amphisomes). Biochem. J. 291 (1993) 115-121
    • (1993) Biochem. J. , vol.291 , pp. 115-121
    • Strømhaug, P.E.1    Seglen, P.O.2
  • 49
    • 34248139628 scopus 로고    scopus 로고
    • Molecular machinery of autophagosome formation in yeast, Saccharomyces cerevisiae
    • Suzuki K., and Ohsumi Y. Molecular machinery of autophagosome formation in yeast, Saccharomyces cerevisiae. FEBS Lett. 581 (2007) 2156-2161
    • (2007) FEBS Lett. , vol.581 , pp. 2156-2161
    • Suzuki, K.1    Ohsumi, Y.2
  • 50
    • 33746108329 scopus 로고    scopus 로고
    • Lysosomal turnover, but not a cellular level, of endogenous LC3 is a marker for autophagy
    • Tanida I., Minematsu-Ikeguchi N., and Kominami E. Lysosomal turnover, but not a cellular level, of endogenous LC3 is a marker for autophagy. Autophagy 1 (2005) 84-91
    • (2005) Autophagy , vol.1 , pp. 84-91
    • Tanida, I.1    Minematsu-Ikeguchi, N.2    Kominami, E.3
  • 52
    • 0022373068 scopus 로고
    • 14C]sucrose: Density gradient distribution of sequestered radioactivity
    • 14C]sucrose: Density gradient distribution of sequestered radioactivity. Eur. J. Biochem. 153 (1985) 223-229
    • (1985) Eur. J. Biochem. , vol.153 , pp. 223-229
    • Tolleshaug, H.1    Seglen, P.O.2
  • 53
    • 0033591236 scopus 로고    scopus 로고
    • Autolysosomal membrane-associated betaine homocysteine methyltransferase: Limited degradation fragment of a sequestered cytosolic enzyme monitoring autophagy
    • Ueno T., Ishidoh K., Mineki R., Tanida I., Murayama K., Kadowaki M., and Kominami E. Autolysosomal membrane-associated betaine homocysteine methyltransferase: Limited degradation fragment of a sequestered cytosolic enzyme monitoring autophagy. J. Biol. Chem. 274 (1999) 15222-15229
    • (1999) J. Biol. Chem. , vol.274 , pp. 15222-15229
    • Ueno, T.1    Ishidoh, K.2    Mineki, R.3    Tanida, I.4    Murayama, K.5    Kadowaki, M.6    Kominami, E.7
  • 55
    • 34249978518 scopus 로고    scopus 로고
    • Autophagy and NF-κB: Fight for fate
    • Xiao G. Autophagy and NF-κB: Fight for fate. Cytokine Growth Factor Rev. 18 (2007) 233-243
    • (2007) Cytokine Growth Factor Rev. , vol.18 , pp. 233-243
    • Xiao, G.1
  • 56
    • 27644484061 scopus 로고    scopus 로고
    • Autophagy: Molecular machinery for self-eating
    • Yorimitsu T., and Klionsky D.J. Autophagy: Molecular machinery for self-eating. Cell Death. Differ. 12 (2005) 1542-1552
    • (2005) Cell Death. Differ. , vol.12 , pp. 1542-1552
    • Yorimitsu, T.1    Klionsky, D.J.2
  • 58
    • 0017071055 scopus 로고
    • Enzyme loading of electrically homogeneous human red blood cell ghosts prepared by dielectric breakdown
    • Zimmermann U., Riemann F., and Pilwat G. Enzyme loading of electrically homogeneous human red blood cell ghosts prepared by dielectric breakdown. Biochim. Biophys. Acta 436 (1976) 460-474
    • (1976) Biochim. Biophys. Acta , vol.436 , pp. 460-474
    • Zimmermann, U.1    Riemann, F.2    Pilwat, G.3
  • 59
    • 0018400880 scopus 로고
    • Rapid separation of particulate and soluble fractions from isolated cell preparations (digitonin and cell cavitation procedures)
    • Zuurendonk P.F., Tischler M.E., Akerboom T.P., Van Der M.R., Williamson J.R., and Tager J.M. Rapid separation of particulate and soluble fractions from isolated cell preparations (digitonin and cell cavitation procedures). Methods Enzymol 56 (1979) 207-223
    • (1979) Methods Enzymol , vol.56 , pp. 207-223
    • Zuurendonk, P.F.1    Tischler, M.E.2    Akerboom, T.P.3    Van Der, M.R.4    Williamson, J.R.5    Tager, J.M.6
  • 61
    • 34250828455 scopus 로고    scopus 로고
    • Proteomic analysis of membrane-associated proteins from rat liver autophagosomes
    • Øverbye A., Fengsrud M., and Seglen P.O. Proteomic analysis of membrane-associated proteins from rat liver autophagosomes. Autophagy 3 (2007) 300-322
    • (2007) Autophagy , vol.3 , pp. 300-322
    • Øverbye, A.1    Fengsrud, M.2    Seglen, P.O.3


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