Chapter 16 Assessing Mammalian Autophagy by WIPI-1/Atg18 Puncta Formation

Methods in Enzymology

Volumn 451, Issue C, 2009, Pages 247-260

  Proikas Cezanne, Tassula ^a   Pfisterer, Simon G ^a  

^a Interfaculty Institute for Cell Biology   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CARRIER PROTEIN; WIPI 1 PROTEIN, HUMAN; WIPI-1 PROTEIN, HUMAN; GREEN FLUORESCENT PROTEIN; PHOSPHATIDYLINOSITOL 3 PHOSPHATE; PHOSPHOLIPID; PROTEIN DERIVATIVE; UNCLASSIFIED DRUG; WIPI 1 PROTEIN;

ARTICLE; AUTOPHAGY; BIOASSAY; CELL LINE; CONFOCAL MICROSCOPY; GENETICS; HELA CELL; HUMAN; METABOLISM; PHYSIOLOGY; TUMOR CELL LINE; CARCINOGENESIS; CELL ACTIVITY; CELL ASSAY; CELL CULTURE; CELL MEMBRANE; CELL VACUOLE; EUKARYOTIC CELL; FLUORESCENCE MICROSCOPY; GENETIC TRANSFECTION; HEART DISEASE; HOMEOSTASIS; HUMAN CELL; IMMUNOFLUORESCENCE; IMMUNOHISTOCHEMISTRY; LIPID TRANSPORT; MEMBRANE COMPONENT; MYOPATHY; NERVE DEGENERATION; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN FAMILY; PROTEIN FUNCTION; REVIEW;

AUTOPHAGY; BIOLOGICAL ASSAY; CARRIER PROTEINS; CELL LINE; CELL LINE, TUMOR; HELA CELLS; HUMANS; MICROSCOPY, CONFOCAL;

EUKARYOTA; MAMMALIA;

EID: 59249106796     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(08)03616-1     Document Type: Review

References (16)

1. Barth H., Meiling-Wesse K., Epple U.D., and Thumm M. Autophagy and the cytoplasm to vacuole targeting pathway both require Aut10p. FEBS Lett. 508 1 (2001) 23-28
2. Dove S.K., Piper R.C., McEwen R.K., Yu J.W., King M.C., Hughes D.C., Thuring J., Holmes A.B., Cooke R.H., Michell R.H., Parker P.J., and Lemmon M.A. Svp1p defines a family of phosphatidylinositol 3,5-bisphosphate effectors. EMBO J. 23 9 (2004) 1922-1933
3. Efe J.A., Botelho R.J., and Emr S.D. Atg18 regulates organelle morphology and Fab1 kinase activity independent of its membrane recruitment by phosphatidylinositol 3,5-bisphosphate. Mol. Biol. Cell 18 11 (2007) 4232-4244
4. Gillooly D.J., Morrow I.C., Lindsay M., Gould R., Bryant N.J., Gaullier J.M., Parton R.G., and Stenmark H. Localization of phosphatidylinositol 3-phosphate in yeast and mammalian cells. EMBO J. 19 17 (2000) 4577-4588
5. Guan J., Stromhaug P.E., George M.D., Habibzadegah-Tari P., Bevan A., Dunn Jr. W.A., and Klionsky D.J. Cvt18/Gsa12 is required for cytoplasm-to-vacuole transport, pexophagy, and autophagy in Saccharomyces cerevisiae and Pichia pastoris. Mol. Biol. Cell. 12 12 (2001) 3821-3838
6. Jeffries, T. R., Dove, S. K., Michell, R. H., and Parker, P. J. PtdIns-specific MPR pathway association of a novel WD40 repeat protein, WIPI49. Mol. Biol. Cell 15 (6), 2652-2663.
7. Klionsky D.J., Abeliovich H., Agostinis P., Agrawal D.K., Aliev G., Askew D.S., Baba M., Baehrecke E.H., Bahr B.A., Ballabio A., Bamber B.A., Bassham D.C., et al. Guidelines for the use and Interpretation of assays for monitoring autophagy in higher eukaryotes. Autophagy 4 2 (2008) 151-175
8. Krick R., Tolstrup J., Appelles A., Henke S., and Thumm M. The relevance of the phosphatidylinositolphosphat-binding motif FRRGT of Atg18 and Atg21 for the Cvt pathway and autophagy. FEBS Lett. 580 19 (2006) 4632-4638
9. Kuma A., Matsui M., and Mizushima N. LC3, an autophagosome marker, can be incorporated into protein aggregates independent of autophagy: Caution in the interpretation of LC3 localization. Autophagy 3 4 (2007) 323-328
10. Mizushima N., and Yoshimori T. How to interpret LC3 immunoblotting. Autophagy 3 6 (2007) 542-545
11. Proikas-Cezanne T., Waddell S., Gaugel A., Frickey T., Lupas A., and Nordheim A. WIPI-1alpha (WIPI49), a member of the novel 7-bladed WIPI protein family, is aberrantly expressed in human cancer and is linked to starvation-induced autophagy. Oncogene 23 58 (2004) 9314-9325
12. Proikas-Cezanne T., Gaugel A., Frickey T., and Nordheim A. Rab14 is part of the early endosomal clathrin-coated TGN microdomain. FEBS Lett. 580 22 (2006) 5241-5246
13. Proikas-Cezanne T., Ruckerbauer S., Stierhof Y.D., Berg C., Nordheim A., and Human A. WIPI-1 puncta-formation: A novel assay to assess mammalian autophagy. FEBS Lett. 581 18 (2007) 3396-3404
14. Strømhaug P.E., Reggiori F., Guan J., Wang C.-W., and Klionsky D.J. Atg21 is a phosphoinositide binding protein required for efficient lipidation and localization of Atg8 during uptake of aminopeptidase I by selective autophagy. Mol. Biol. Cell. 15 8 (2004) 3553-3566
15. Waddell S., Jenkins J.R., and Proikas-Cezanne T.A. "No-hybrids" screen for functional antagonizers of human p53 transactivator function: Dominant negativity in fission yeast. Oncogene 20 42 (2001) 6001-6008
16. Xie Z., and Klionsky D.J. Autophagosome formation: Core machinery and adaptations. Nat. Cell Biol. 9 10 (2007) 1102-1109