An in vitro FRET-based assay for the analysis of SUMO conjugation and isopeptidase cleavage

Methods in Molecular Biology

Volumn 497, Issue , 2009, Pages 241-251

  Stankovic Valentin, Nicolas ^a   Kozaczkiewicz, Lukasz ^a   Curth, Katja ^a   Melchior, Frauke ^a  

^a UNIVERSITY OF GÖTTINGEN   (Germany)

Author keywords

Aos1 Uba2; E1 activating enzyme; E2 conjugating enzyme; Fluorescence resonance energy transfer; FRET; Isopeptidase; SUMO; Ubc9; Yellow fluorescent protein

Indexed keywords

HYBRID PROTEIN; ISOPEPTIDASE; LYASE; SUMO 1 PROTEIN; UNCLASSIFIED DRUG;

ANIMAL; BIOLOGICAL MODEL; CHEMISTRY; DIAGNOSIS, MEASUREMENT AND ANALYSIS; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMAN; METABOLISM; METHODOLOGY; PROTEIN PROCESSING; REVIEW;

ANIMALS; CARBON-NITROGEN LYASES; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMANS; LABORATORY TECHNIQUES AND PROCEDURES; MODELS, BIOLOGICAL; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT FUSION PROTEINS; SUMO-1 PROTEIN;

EID: 59249088923     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-566-4_16     Document Type: Article

References (8)

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