Structures and reaction pathways of the molybdenum centres of sulfite-oxidizing enzymes by pulsed EPR spectroscopy

Biochemical Society Transactions

Volumn 36, Issue 6, 2008, Pages 1129-1133

  Enemark, John H ^a   Astashkin, Andrei V ^a   Raitsimring, Arnold M ^a  

^a The University of Arizona   (United States)

Author keywords

Molybdenum centre; Pulsed EPR spectroscopy; Sulfite oxidizing enzyme (SOE)

Indexed keywords

MOLYBDENUM; OXIDOREDUCTASE; SULFITE OXIDIZING ENZYME; UNCLASSIFIED DRUG; CHLORIDE; LIGAND; OXYGEN; SULFITE OXIDASE; SULFUR;

ARTICLE; CATALYSIS; CHEMICAL REACTION; ELECTRON SPIN RESONANCE; ENZYME ACTIVITY; ENZYME STRUCTURE; GEOMETRY; ISOTOPE LABELING; PRIORITY JOURNAL; SPECTROSCOPY; STRUCTURE ANALYSIS; CHEMISTRY; HUMAN; METABOLISM; PH;

CATALYSIS; CHLORIDES; ELECTRON SPIN RESONANCE SPECTROSCOPY; HUMANS; HYDROGEN-ION CONCENTRATION; ISOTOPE LABELING; LIGANDS; MOLYBDENUM; OXYGEN ISOTOPES; SULFITE OXIDASE; SULFUR ISOTOPES;

EID: 59149103868     PISSN: 03005127     EISSN: 14708752     Source Type: Journal    
DOI: 10.1042/BST0361129     Document Type: Article

References (21)

1. Kisker, C., Schindelin, H., Pacheco, A., Wehbi, W., Garrett, R.M., Rajagopalan, K.V., Enemark, J.H. and Rees, D.C. (1997) Molecular basis of sulfite oxidase defficiency from the structure of sulfite oxidase. Cell 91, 973-983
2. Schrader, N., Fischer, K., Theis, K., Mendel, R.R., Schwarz, G. and Kisker, C. (2003) The crystal structure of plant sulfite oxidase provides insights into sulfite oxidation in plants and animals. Structure 11, 1251-1263
3. Kappler, U. and Bailey, S. (2005) Molecular basis of intramolecular electron transfer in sulfite-oxidizing enzymes is revealed by high resolution structure of a heterodimeric complex of the catalytic molybdopterin subunit and a c-type cytochrome subunit. J. Biol. Chem. 280, 24999-25007
4. Hille, R. (1996) The mononuclear molybdenum enzymes. Chem. Rev. 96, 2757-2816
5. Lamy, M.T., Gutteridge, S. and Bray, R.C. (1980) Electron paramagnetic resonance parameters of molybdenum(V) in sulphite oxidase from chicken liver. Biochem. J. 185, 397-403
6. Gutteridge, S., Lamy, M.T. and Bray, R.C. (1980) The nature of the phosphate inhibitor complex of sulphite oxidase from electron paramagnetic resonance studies using oxygen-17. Biochem. J. 191, 285-288
7. Bray, R.C., Gutteridge, S., Lamy, M.T. and Wilkinson, T. (1983) Equilibria amongst different molybdenum(V)-containing species from sulphite oxidase: evidence for a halide ligand of molybdenum in the low pH species. Biochem. J. 211, 227-236
8. Enemark, J.H., Astashkin, A.V. and Raitsimring, A.M. (2006) Investigation of the coordination structures of the molybdenum(V) sites of sulfite oxidizing enzymes by pulsed EPR spectroscopy. Dalton Trans., 3501-3514
9. 33S labeling and ESEEM spectroscopy. J. Am. Chem. Soc. 129, 14800-14810
10. 33S labeling. J. Am. Chem. Soc. 130, 8471-8480
11. Doonan, C.J., Wilson H.L., Rajagopalan, K.V., Garrett, R.M., Bennett, B., Prince, R.C. and George, G.N. (2008) Modified active site coordination in a clinical mutant of sul.te oxidase. J. Am. Chem. Soc. 130, 6298-6305
12. -. Biochem. Biophys. Res. Commun. 91, 434-439
13. 17O ESEEM evidence for exchange of the axial oxo ligand in the molybdenum center of the high pH form of sulfite oxidase. J. Am. Chem. Soc. 127, 502-503
14. -. J. Am. Chem. Soc. 127, 16713-16722
15. 17O pulsed electron nuclear double resonance study of gadolinium complexes with water. J. Phys. Chem. A 108, 7318-7323
16. V electron paramagnetic resonance of sulfite oxidase revisited: the low-pH chloride signal. Inorg. Chem. 47, 2033-2038
17. 4-band ESEEM of equatorial chloro ligands in oxomolybdenum(V) complexes. J. Inorg. Biochem. 101, 1623-1629
18. 17O-NMR studies of molybdoenzyme model complexes. J. Biol. Inorg. Chem. 12 (Suppl. 1), S80
19. Tanford, C. and Roxby, R. (1972) Interpretation of protein titration curves: application to lysozyme. Biochemistry 1972, 2192-2198
20. Bashford, D. and Karplus, M. (1991) Multiple-site titration curves of proteins: an analysis of exact and approximate methods for their calculation J. Phys. Chem. 95, 9556-9561
21. Astashkin, A.V., Hood, B.L., Feng, C., Hille, R., Mendel, R.R., Raitsimring, A.M. and Enemark, J.H. (2005) Structures of the Mo(V) forms of sulfite oxidase from Arabidopsis thaliana by pulsed EPR spectroscopy. Biochemistry 44, 13274-13281