Ser649 and Ser650 are the major determinants of protein kinase A-mediated activation of human hormone-sensitive lipase against lipid substrates

PLoS ONE

Volumn 3, Issue 11, 2008, Pages

  Krintel, Christian ^a,b   Osmark, Peter ^a   Larsen, Martin R ^c   Resjö, Svante ^a   Logan, Derek T ^b   Holm, Cecilia ^a  

^a LUND UNIVERSITY   (Sweden)

^b LUND UNIVERSITY   (Sweden)

^c UNIVERSITY OF SOUTHERN DENMARK   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

4 NITROPHENOL; 4 NITROPHENOL BUTYRIC ACID; ALANINE; CYCLIC AMP DEPENDENT PROTEIN KINASE; GLUTAMINE; HISTIDINE; LIPID; SERINE; TRIACYLGLYCEROL LIPASE; TRIOLEIN; UNCLASSIFIED DRUG; CHOLESTEROL ESTERASE; FATTY ACID; TRIACYLGLYCEROL;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME PHOSPHORYLATION; ENZYME PURIFICATION; HUMAN; IN VITRO STUDY; INSECT CELL; LIPOLYSIS; MASS SPECTROMETRY; MUTANT; NONHUMAN; PROTEIN EXPRESSION; SITE DIRECTED MUTAGENESIS; WILD TYPE; ANIMAL; CHEMISTRY; GENE VECTOR; INSECT; LIQUID CHROMATOGRAPHY; METABOLISM; METHODOLOGY; PHOSPHORYLATION; RAT;

HEXAPODA; RATTUS;

ANIMALS; CHROMATOGRAPHY, LIQUID; CYCLIC AMP-DEPENDENT PROTEIN KINASES; ENZYME ACTIVATION; FATTY ACIDS; GENETIC VECTORS; HUMANS; INSECTS; LIPIDS; PHOSPHORYLATION; RATS; SERINE; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; STEROL ESTERASE; TRIGLYCERIDES;

EID: 59149100574     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0003756     Document Type: Article

References (33)

1. Schweiger M, Schreiber R, Haemmerle G, Lass A, Fledelius C, et al. (2006) Adipose triglyceride lipase and hormone-sensitive lipase are the major enzymes in adipose tissue triacylglycerol catabolism. J Biol Chem 281: 40236-40241.
2. Stralfors P, Belfrage P (1983) Phosphorylation of hormone-sensitive lipase by cyclic AMP-dependent protein kinase. J Biol Chem 258: 15146-15152.
3. Garton AJ, Yeaman SJ (1990) Identification and role of the basal phosphorylation site on hormone-sensitive lipase. Eur J Biochem 191: 245-250.
4. Garton AJ, Campbell DG, Carling D, Hardie DG, Colbran RJ, et al. (1989) Phosphorylation of bovine hormone-sensitive lipase by the AMP-activated protein kinase. A possible antilipolytic mechanism. Eur J Biochem 179: 249-254.
5. Feller G, Thiry M, Gerday C (1991) Nucleotide sequence of the lipase gene lip2 from the antarctic psychrotroph Moraxella TA144 and site-specific mutagenesis of the conserved serine and histidine residues. DNA Cell Biol 10: 381-388.
6. Belfrage P, Fredrikson G, Olsson H, Stralfors P (1984) Regulation of adipose tissue lipolysis through reversible phosphorylation of hormone-sensitive lipase. Adv Cyclic Nucleotide Protein Phosphorylation Res 17: 351-359.
7. Holm C, Belfrage P, Osterlund T, Davis RC, Schotz MC, et al. (1994) Hormone-sensitive lipase: structure, function, evolution and overproduction in insect cells using the baculovirus expression system. Protein Eng 7: 537-541.
8. Contreras JA, Danielsson B, Johansson C, Osterlund T, Langin D, et al. (1998) Human hormone-sensitive lipase: expression and large-scale purification from a baculovirus/insect cell system. Protein Expr Purif 12: 93-99.
9. Anthonsen MW, Ronnstrand L, Wernstedt C, Degerman E, Holm C (1998) Identification of novel phosphorylation sites in hormone-sensitive lipase that are phosphorylated in response to isoproterenol and govern activation properties in vitro. J Biol Chem 273: 215-221.
10. Su CL, Sztalryd C, Contreras JA, Holm C, Kimmel AR, et al. (2003) Mutational analysis of the hormone-sensitive lipase translocation reaction in adipocytes. J Biol Chem 278: 43615-43619.
11. Shen WJ, Patel S, Natu V, Kraemer FB (1998) Mutational analysis of structural features of rat hormone-sensitive lipase. Biochemistry 37: 8973-8979.
12. Watt MJ, Heigenhauser GJ, Spriet LL (2003) Effects of dynamic exercise intensity on the activation of hormone-sensitive lipase in human skeletal muscle. J Physiol 547: 301-308.
13. Talanian JL, Tunstall RJ, Watt MJ, Duong M, Perry CG, et al. (2006) Adrenergic regulation of HSL serine phosphorylation and activity in human skeletal muscle during the onset of exercise. Am J Physiol Regul Integr Comp Physiol 291: R1094-1099.
14. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.
15. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
16. Larsen MR, Thingholm TE, Jensen ON, Roepstorff P, Jorgensen TJ (2005) Highly selective enrichment of phosphorylated peptides from peptide mixtures using titanium dioxide microcolumns. Mol Cell Proteomics 4: 873-886.
17. Jensen SS, Larsen MR (2007) Evaluation of the impact of some experimental procedures on different phosphopeptide enrichment techniques. Rapid Commun Mass Spectrom 21: 3635-3645.
18. Thingholm TE, Jorgensen TJ, Jensen ON, Larsen MR (2006) Highly selective enrichment of phosphorylated peptides using titanium dioxide. Nat Protoc 1: 1929-1935.
19. Kjellstrom S, Jensen ON (2004) Phosphoric acid as a matrix additive for MALDI MS analysis of phosphopeptides and phosphoproteins. Anal Chem 76: 5109-5117.
20. Schroeder MJ, Shabanowitz J, Schwartz JC, Hunt DF, Coon JJ (2004) A neutral loss activation method for improved phosphopeptide sequence analysis by quadrupole ion trap mass spectrometry. Anal Chem 76: 3590-3598.
21. Tornqvist H, Krabisch L, Belfrage P (1972) Rapid assay for hormone-sensitive lipase activity of adipose tissue. J Lipid Res 13: 424-426.
22. Tornqvist H, Bjorgell P, Krabisch L, Belfrage P (1978) Monoacylmonoalkylglycerol as a substrate for diacylglycerol hydrolase activity in adipose tissue. J Lipid Res 19: 654-656.
23. Osterlund T, Danielsson B, Degerman E, Contreras JA, Edgren G, et al. (1996) Domain-structure analysis of recombinant rat hormone-sensitive lipase. Biochem J 319 (Pt 2): 411-420.
24. Fredrikson G, Stralfors P, Nilsson NO, Belfrage P (1981) Hormone-sensitive lipase of rat adipose tissue. Purification and some properties. J Biol Chem 256: 6311-6320.
25. Ben Ali Y, Chahinian H, Petry S, Muller G, Carriere F, et al. (2004) Might the kinetic behavior of hormone-sensitive lipase reflect the absence of the lid domain? Biochemistry 43: 9298-9306.
26. Chahinian H, Ali YB, Abousalham A, Petry S, Mandrich L, et al. (2005) Substrate specificity and kinetic properties of enzymes belonging to the hormone-sensitive lipase family: comparison with non-lipolytic and lipolytic carboxylesterases. Biochim Biophys Acta 1738: 29-36.
27. Wang J, Shen WJ, Patel S, Harada K, Kraemer FB (2005) Mutational analysis of the "regulatory module" of hormone-sensitive lipase. Biochemistry 44: 1953-1959.
28. Osterlund T, Beussman DJ, Julenius K, Poon PH, Linse S, et al. (1999) Domain identification of hormone-sensitive lipase by circular dichroism and fluorescence spectroscopy, limited proteolysis, and mass spectrometry. J Biol Chem 274: 15382-15388.
29. Watt MJ, Holmes AG, Pinnamaneni SK, Garnham AP, Steinberg GR, et al. (2006) Regulation of HSL serine phosphorylation in skeletal muscle and adipose tissue. Am J Physiol Endocrinol Metab 290: E500-508.
30. Nilsson NO, Stralfors P, Fredrikson G, Belfrage P (1980) Regulation of adipose tissue lipolysis: effects of noradrenaline and insulin on phosphorylation of hormone-sensitive lipase and on lipolysis in intact rat adipocytes. FEBS Lett 111: 125-130.
31. Holm C, Contreras JA, Verger R, Schotz MC (1997) Large-scale purification and kinetic properties of recombinant hormone-sensitive lipase from baculovirus-insect cell systems. Methods Enzymol 284: 272-284.
32. Cook KG, Yeaman SJ, Stralfors P, Fredrikson G, Belfrage P (1982) Direct evidence that cholesterol ester hydrolase from adrenal cortex is the same enzyme as hormone-sensitive lipase from adipose tissue. Eur J Biochem 125: 245-249.
33. Cook KG, Colbran RJ, Snee J, Yeaman SJ (1983) Cytosolic cholesterol ester hydrolase from bovine corpus luteum. Its purification, identification, and relationship to hormone-sensitive lipase. Biochim Biophys Acta 752: 46-53.