Expression and purification the antimicrobial peptide CM4 in Escherichia coli

Biotechnology Letters

Volumn 31, Issue 3, 2009, Pages 437-441

  Zhou, Liangfan ^a   Lin, Qingping ^a   Li, Baocun ^a   Li, Nannan ^a   Zhang, Shuangquan ^a  

^a NANJING NORMAL UNIVERSITY   (China)

Author keywords

Antimicrobial peptide CM4; Escherichia coli; Fusion expression hydroxylamine hydrochloride

Indexed keywords

CHROMATOGRAPHIC ANALYSIS; CHROMATOGRAPHY; ESCHERICHIA COLI; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; PURIFICATION;

ANTI-MICROBIAL ACTIVITIES; ANTIMICROBIAL PEPTIDE CM4; CATIONIC PEPTIDES; EXPRESSION VECTORS; FUSION EXPRESSION HYDROXYLAMINE HYDROCHLORIDE; FUSION PROTEINS; NEW APPROACHES;

AMINES;

ANTIINFECTIVE AGENT; ANTIMICROBIAL CATIONIC PEPTIDE; CM4 PEPTIDE, BOMBYX MORI; HYBRID PROTEIN; HYDROXYLAMINE;

AFFINITY CHROMATOGRAPHY; ARTICLE; BIOSYNTHESIS; DRUG EFFECT; ESCHERICHIA COLI; FUNGUS; GENE VECTOR; GENETICS; GRAM NEGATIVE BACTERIUM; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; METABOLISM; MICROBIOLOGICAL EXAMINATION;

ANTI-INFECTIVE AGENTS; ANTIMICROBIAL CATIONIC PEPTIDES; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ESCHERICHIA COLI; FUNGI; GENETIC VECTORS; GRAM-NEGATIVE BACTERIA; HYDROXYLAMINE; MICROBIAL SENSITIVITY TESTS; RECOMBINANT FUSION PROTEINS;

ESCHERICHIA COLI;

EID: 59149097550     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-008-9893-0     Document Type: Article

References (11)

1. W Cao Y Zhou Y Ma Q Luo D Wei 2005 Expression and purification of antimicrobial peptide adenoregulin with C-amidated terminus in Escherichia coli Protein Expr Purif 40 404 410
2. YQ Chen SQ Zhang BC Li W Qiu B Jiao J Zhang ZY Diao 2008 Expression of a cytotoxic cationic antibacterial peptide in Escherichia coli using two fusion partners Protein Expr Purif 57 303 311
3. BL Kagan ME Selsted T Ganz RI Lehrer 1990 Antimicrobial defensin peptides form voltage-dependent ion-permeable channels in planar lipid bilayer membranes Proc Natl Acad Sci USA 87 210 214
4. DG Lee Y Park HN Kim HK Kim PI Kim BH Choi KS Hahm 2002 Antifungal mechanism of an antimicrobial peptide, HP (2-20), derived from N-terminus of Helicobacter pylori ribosomal protein L1 against Candida albicans Biochem Biophys Res Commun 291 1006 1013
5. Y Liang JX Wang XF Zhao XJ Du JF Xue 2006 Molecular cloning and characterization of cecropin from the housefly (Musca domestica), and its expression in Escherichia coli Dev Comp Immunol 30 249 257
6. A Makovitzki D Avrahami Y Shai 2006 Ultrashort antibacterial and antifungal lipopeptides Proc Natl Acad Sci USA 103 15997 16002
7. T Moks L Abrahmsen E Holmgren M Bilich A Olsson M Uhlen G Pohl C Sterky H Hultberg S Josephson 1987 Expression of human insulin-like growth factor I in bacteria: use of optimized gene fusion vectors to facilitate protein purification Biochemistry 26 5239 5244
8. C Prodromou LH Pearl 1992 Recursive PCR: a novel technique for total gene synthesis Protein Eng 5 827 829
9. H Sugiarto PL Yu 2004 Avian antimicrobial peptides: the defense role of beta-defensins Biochem Biophys Res Commun 323 721 727
10. T Yizeng SQ Zhang XM Qu 1989 Separation, purification of antibacterial CM4 and the research of the structure and character Sci China 32 473 488
11. SQ Zhang HW Jia ZY Dai 1997 Ultrastructure observation of K562 leukemia cells treated with antibacterial peptide CM4 component Prog Biochem Biophys 24 159 163