Induction of cholinergic differentiation by 5-azacytidine in NG108-15 neuronal cells

NeuroReport

Volumn 20, Issue 2, 2009, Pages 157-160

  Aizawa, Shu ^a   Sensui, Naoto ^a   Yamamuro, Yutaka ^a,b  

^a Nihon University   (Japan)

^b NIHON UNIVERSITY   (Japan)

Author keywords

5 azacytidine; Choline acetyltransferase; DNA methylation; Neuronal differentiation; NG108 15

Indexed keywords

5 METHYLCYTOSINE; AZACITIDINE; CHOLINE ACETYLTRANSFERASE; DNA; DNA METHYLTRANSFERASE; MESSENGER RNA; NEUROFILAMENT PROTEIN;

ANIMAL CELL; ARTICLE; CELL LINE; CELL PROLIFERATION; CHOLINERGIC NERVE CELL; CONTROLLED STUDY; DEMETHYLATION; DOSE RESPONSE; DRUG MECHANISM; ENZYME ACTIVITY; GENE EXPRESSION; NERVE CELL DIFFERENTIATION; NERVE FIBER GROWTH; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN FUNCTION; ANALYSIS OF VARIANCE; ANIMAL; CELL DIFFERENTIATION; CYTOLOGY; DENSITOMETRY; DNA METHYLATION; DRUG EFFECT; GENETICS; METABOLISM; MOUSE; NERVE CELL; NEURITE; PHYSIOLOGY; POLYMERASE CHAIN REACTION; RAT; TUMOR CELL LINE; WESTERN BLOTTING;

ANALYSIS OF VARIANCE; ANIMALS; AZACITIDINE; BLOTTING, WESTERN; CELL DIFFERENTIATION; CELL LINE, TUMOR; CHOLINE O-ACETYLTRANSFERASE; DENSITOMETRY; DNA METHYLATION; GENE EXPRESSION; MICE; NEURITES; NEUROFILAMENT PROTEINS; NEURONS; POLYMERASE CHAIN REACTION; RATS; RNA, MESSENGER;

EID: 59149094466     PISSN: 09594965     EISSN: None     Source Type: Journal    
DOI: 10.1097/WNR.0b013e32831d28ee     Document Type: Article

References (22)

1. Nan X, Campoy FJ, Bird A. MeCP2 is a transcriptional repressor with abundant binding sites in genomic chromatin. Cell 1997; 88:471-481.
2. Nan X, Ng HH, Johnson CA, Laherty CD, Turner BM, Eisenman RN, et al. Transcriptional repression by the methyl-CpG-binding protein MeCP2 involves a histone deacetylase complex. Nature 1998; 393: 386-389.
3. Ng HH, Zhang Y, Hendrich B, Johnson CA, Tumer BM, Erdjument-Bromage H, et al. MBD2 is a transcriptional repressor belonging to the MeCP2 histone deacetylase complex. Nat Genet 1999; 23:58-61.
4. Tate PH, Bird AP. Effects of DNA methylation on DNA-binding proteins and gene expression. Curr Opin Genet Dev 1993; 3:226-231.
5. Wade PA, Gegonne A, Jones PL, Ballestar E, Aubry F, Wolffe AP. Mi-2 complex couples DNA methylation to chromatin remodeling and histone deacetylation. Nat Genet 1999; 23:62-66.
6. Bird AP, Wolffe AP. Methylation-induced repression - belts, braces, and chromatin. Cell 1999; 99:451-454.
7. Jones PA, Takai D. The role of DNA methylation in mammalian epigenetics. Science 2001; 293:1068-1070.
8. Tucker KL. Methylated cytosine and the brain: a new base for neuroscience. Neuron 2001; 30:649-652.
9. Feng J, Fouse S, Fan G. Epigenetic regulation of neuronal gene expression and neuronal function. Pediatr Res 2007; 61:58-63.
10. Christman JK. 5-a2acytidine and 5-aza-2'-deoxycytidine as inhibitors of DNA methylation: mechanistic studies and their implications for cancer therapy. Oncogene 2002; 21:5483-5495.
11. Lyko F, Brown R. DNA methyltransferase inhibitors and the development of epigenetic cancer therapies. J Natl Cancer Inst 2005; 97:1498-1506.
12. Krystosec A. Neurite formation by neuroblastoma-glioma hybrid cells (NG108-15) in defined medium: stochastic inhibition with persistence of differentiated functions. J Cell Physiol 1985; 125:319-329.
13. Nirenberg M, Wilson S, Higashida H, Rotter A, Krueger K, Busis N, et al. Modulation of synapse formation by cyclic adenosine monophosphate. Science 1983; 222:794-799.
14. Dolezal V, Castell X, Tomasi M, Diebler MR Stimuli that induce a cholinergic neuronal phenotype of NG108-15 cells upregulate ChAT and VAChT mRNAs but fail to increase VAChT protein. Brain Res Bull 2001; 54:363-373.
15. Hamprecht B. Structural, electrophysiological, biochemical, and pharmacological properties of neuroblastoma cell hybrids in cell culture. Int Rev Cytol 1977; 49:99-170.
16. Higashida H. Acetylcholine release by bradykinin, inositol 1,4,5-triphosphate and phorbol dibutyrate in rodent neuroblastoma cells. J Physiol 1988; 397:209-222.
17. McGee R, Simpson P, Christian C, Mata M, Nelson P, Niremberg M. Regulation of acetylcholine release from neuroblastoma x glioma hybrid cell lines. Proc Natl Acad Sci U S A 1978; 75:1314-1318.
18. Aizawa S, Yamamuro Y. Estradiol regulates alternative splicing of estrogen receptor-alpha mRNA in differentiated NG 108-15 cells. Life Sci 2008; 82:692-698.
19. Misawa H, Takahashi R, Deguchi T. Gene expression of mouse choline acetyltransferase. J Biol Chem 1992; 267:20392-20399.
20. Pu H, Zhai P, Gurney M. Enhancer, silencer, and growth factor responsive regulatory sequences in the promoter for the mouse choline acetyltransferase gene. Mol Cell Neurosci 1993; 4:131-142.
21. Bignami F, Bevilacqua P, Biagioni S, De Jaco A, Casamenti F, Felsani A, et al. Cellular acetylcholine content and neuronal differentiation. J Neurochem 1997; 69:1374-1381.
22. Anelli T, Mannello F, Salani M, Tonti GA, Poiana G, Biagioni S. Acetylcholine induces neurite outgrowth and modulates matrix metalloproteinase 2 and 9. Biochem Biophys Res Commun 2007; 362:269-274.