Systematic determination of the peptide acceptor preferences for the human UDP-Gal:glycoprotein-α-GalNAc β3 galactosyltranferase (T-synthase)

Glycobiology

Volumn 19, Issue 3, 2009, Pages 321-328

  Perrine, Cynthia ^a   Ju, Tongzhong ^b   Cummings, Richard D ^b   Gerken, Thomas A ^a  

^a CASE WESTERN RESERVE UNIVERSITY   (United States)

^b EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Core 1 transferase; O glycosylation; Sequence motifs; T synthase; UDP Gal:glycoprotein GalNAc 3 galactosyltransferase

Indexed keywords

ALPHA N ACETYLGLUCOSAMINIDASE; ARGININE; GLYCINE; GLYCOPEPTIDE; GLYCOSYLATED PROTEIN; HISTIDINE; LYSINE; PHENYLALANINE; TYROSINE; URIDINE DIPHOSPHATE GALACTOSE;

ARTICLE; BIOCHEMICAL COMPOSITION; BIOSYNTHESIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME GLYCOSYLATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE; HUMAN; MOLECULAR INTERACTION; NUCLEOTIDE SEQUENCE; PEANUT; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PROCESSING; PROTEIN STRUCTURE; PROTEIN TARGETING; SEQUENCE ANALYSIS;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; GALACTOSYLTRANSFERASES; GLYCOPEPTIDES; HUMANS; N-ACETYLGALACTOSAMINYLTRANSFERASES; PEPTIDES; URIDINE DIPHOSPHATE;

ARACHIS HYPOGAEA;

EID: 58949086661     PISSN: 09596658     EISSN: 14602423     Source Type: Journal    
DOI: 10.1093/glycob/cwn143     Document Type: Article

References (22)

1. Berger EG. 1999. Tn-syndrome. Biochimica et Biophysica Acta. 1455:255-268.
2. Brockhausen I. 1997. Biosynthesis and functions of O-glycans and regulation of mucin antigen expression in cancer. Biochem Soc Trans 25:871-874.
3. Brockhausen I. 1999. Pathways of O-glycan biosynthesis in cancer cells. Biochim Biophys Acta. 1473:67-95.
4. Brockhausen I, Moller G, Merz G, Adermann K, Paulsen H. 1990. Control of mucin synthesis: The peptide portion of synthetic O-glycopeptide substrates influences the activity of O-glycan core 1 UDP-galactose: N-acetyl-α-galactosaminyl-R α 3-galactosyltransferase. Biochemistry. 29:10206-10212.
5. Freire T, Bay S, Mensdorff-Pouilly S, Osinaga E. 2005. Molecular basis of incomplete O-glycan synthesis in MCF-7 breast cancer cells: Putative role of MUC6 in Tn antigen expression. Cancer Res. 65:7880-7887.
6. Gerken TA. 2004. Kinetic modeling confirms the biosynthesis of mucin Core 1 (α-Gal(1-3) a-GalNAc-O-Ser/Thr) O-glycan structures are modulated by neighboring glycosylation effects. Biochemistry. 43:4137-4142.
7. Gerken TA, Gilmore M, Zhang J. 2002. Determination of the site-specific oligosaccharide distribution of the O-glycans attached to the porcine sub-maxillary mucin tandem repeat: Further evidence for the modulation of O-glycan side chain structures by peptide sequence. J Biol Chem. 277:7736-7751.
8. Gerken TA, Ten Hagen KG, Jamison O. 2008. Conservation of peptide acceptor preferences between Drosophila and mammalian polypeptide-GalNAc transferase orthologue pairs. Glycobiology. 18:861-870.
9. Gerken TA, Raman J, Fritz TA, Jamison O. 2006. Identification of common and unique peptide substrate preferences for the UDP-GalNAc:pOlypeptide α-N-acetylgalactosaminyltransferases T1 & T2 (ppGalNAc T1 & T2) derived from oriented random peptide substrates. J Biol Chem. 281:32403-32416.
10. Gerken TA, Tep C, Rarick J. 2004. Role of peptide sequence and neighboring residue glycosylation on the substrate specificity of the uridine 5′-diphosphate-α-N-acetylgalactosamine: Polypeptide N-acetylgalactosaminyl transferases T1 and T2: Kinetic modeling of the porcine and canine submaxillary gland mucin tandem repeats. Biochemistry. 43:9888-9900.
11. Granovsky M, Bielfeldt T, Peters S, Paulsen H, Meldal M, Brockhausen J, Brockhausen I. 1994. UDPgalactose:gLycoprotein-N acetyl-D-galactosamine 3-β-D-galactosyltransferase activity synthesizing O-glycan core 1 is controlled by the amino acid sequence and glycosylation of glycopeptide substrates. Eur J Biochem 221:1039-1046.
12. Ju T, Aryal RP, Stowell CJ, Cummings RD. 2008. Regulation of protein O-glycosylation by the endoplasmic reticulum-localized molecular chaperone Cosmc. J Cell Biol. 182:531-542.
13. Ju T, Brewer K, D'Souza A, Cummings RD, Canfield WM. 2002. Cloning and expression of human Core 1 β 1,3-galactosyltransferase. J Biol Chem 277:178-186.
14. Ju T, Cummings RD. 2005. Protein glycosylation: Chaperone mutation in Tn syndrome. Nature. 437:1252.
15. Kato K, Jeanneau C, Tarp MA, Benet-Pages A, Lorenz-Depiereux B, Bennett EP, Mandel U, Strom TM, Clausen H. 2006. Polypeptide GaINAc-transferase T3 and familial tumoral calcinosis: Secretion of FGF23 requires O glycosylation. J Biol Chem. 281:18370-18377.
16. Li GS, Zhang H, Lv JC, Shen Y, Wang HY. 2007. Variants of C1GALT1 gene are associated with the genetic susceptibility to IgA nephropathy. Kidney Int. 71:448-453.
17. Liu W, Ramachandran V, Kang J, Kishimoto TK, Cummings RD, McEver RP. 1998. Identitication of N-terminal residues on P-selectin glycoprotein ligand-1 required for binding to P-selectin. J Biol Chem 273:7078-7087.
18. Lotan R, Skutelsky E, Danon D, Sharon N. 1975. The purification, composition, and specificity of the anti-T lectin from peanut ( Arachis hypogaea). J Biol Chem. 250:8518-8523.
19. Pinho S, Marcos NT, Ferreira B, Carvalho AS, Oliveira MJ, Santos-Silva F, Harduin-Lepers A, Reis CA. 2007. Biological significance of cancer-associated sialyl-Tn antigen: Modulation of malignant phenotype in gastric carcinoma cells. Cancer Lett. 249:157-170.
20. Smith AC, de Wolff JF, Molyneux K, Feehally J, Barratt J. 2006. O Glycosylation of serum IgD in IgA nephropathy. J Am Soc Nephrol 17:1192-1199.
21. Springer GF. 1997. Immunoreactive T and Tn epitopes in cancer diagnosis, prognosis, and immunotherapy. J Mol Med. 75:594-602.
22. Xia L, Ju T,Westmuckett A, An G, Ivanciu L, McDaniel JM, Lupu F, Cummings RD, McEver RP. 2004. Defective angiogenesis and fatal embryonic hemorrhage in mice lacking core 1-derived O-glycans. J Cell Biol. 164:451-459.