Mouse Bsep ATPase assay: A nonradioactive tool for assessment of the cholestatic potential of drugs

Journal of Biomolecular Screening

Volumn 14, Issue 1, 2009, Pages 10-15

  Kis, Emese ^a   Rajnai, Zsuzsanna ^a   Ioja, Eniko ^a   Herédi Szabó, Krisztina ^a   Nagy, Tünde ^a   Méhn, Dóra ^a   Krajcsi, Péter ^a,b  

^a SOLVO BIOTECHNOLOGY   (Hungary)

^b NONE   (Hungary)

Author keywords

ATPase activity; Bsep ABCB11; Cholestasis; Mouse; Nonradioactive; Taurochenodeoxycholate

Indexed keywords

ADENOSINE TRIPHOSPHATE; BACULOVIRUS VECTOR; BILE SALT; CYCLOSPORIN A; GLIBENCLAMIDE; KETOCONAZOLE; MBSEP PROTEIN; MEMBRANE PROTEIN; RIFAMPICIN; RIFAMYCIN; TAUROCHENODEOXYCHOLIC ACID; TAUROCHOLIC ACID; TROGLITAZONE; UNCLASSIFIED DRUG; VESICULAR TRANSPORT PROTEIN;

ARTICLE; BACULOVIRUS; CHOLESTASIS; CHOLESTEROL TRANSPORT; DATA ANALYSIS; DRUG SCREENING; ENZYME ACTIVATION; ENZYME ASSAY; ENZYME INHIBITION; ENZYME SUBSTRATE; HIGH THROUGHPUT SCREENING; IC 50; INSECT CELL; MEMBRANE POTENTIAL; MEMBRANE VESICLE; NONHUMAN; ORTHOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN TRANSPORT; WESTERN BLOTTING;

ADENOSINE TRIPHOSPHATASES; ANIMALS; ATP-BINDING CASSETTE TRANSPORTERS; BIOLOGICAL TRANSPORT; CELL LINE; CHOLESTASIS; CHOLESTEROL; MICE; RADIOLIGAND ASSAY; SPODOPTERA;

HEXAPODA;

EID: 58849126967     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057108326145     Document Type: Article

References (18)

1. Meier PJ, St. Meier-Abt A, Barrett C, Boyer JL: Mechanisms of taurocholate transport in canalicular and basolateral rat liver plasma membrane vesicles. Evidence for an electrogenic canalicular organic anion carrier. J Biol Chem 1984 ; 259: 10614-10622.
2. St-Pierre MV, Kullak-Ublick GA, Hagenbuch B., Meier PJ: Transport of bile acids in hepatic and non-hepatic tissues. J Exp Biol 2001 ; 204 (Pt 10). 1673-1686.
3. Schuetz EG, Strom S., Yasuda K., Lecureur V., Assem M., Brimer C.,, et al. Disrupted bile acid homeostasis reveals an unexpected interaction among nuclear hormone receptors, transporters, and cytochrome P450. J Biol Chem 2001 ; 276: 39411-39418.
4. Strautnieks SS, Bull LN, Knisely AS, Kocoshis SA, Dahl N., Arnell H., et al. A gene encoding a liver-specific ABC transporter is mutated in progressive familial intrahepatic cholestasis. Nat Genet 1998 ; 20: 233-238.
5. Noe J., Stieger B., Meier PJ: Functional expression of the canalicular bile salt export pump of human liver. Gastroenterology 2002 ; 123: 1659-1666.
6. Kostrubsky SE, Strom SC, Kalgutkar AS, Kulkarni S., Atherton J., Mireles R.,, et al. Inhibition of hepatobiliary transport as a predictive method for clinical hepatotoxicity of nefazodone. Toxicol Sci 2006 ; 90: 451-459.
7. Sarkadi B., Price EM, Boucher RC, Germann UA, Scarborough GA: Expression of the human multidrug resistance cDNA in insect cells generates a high activity drug-stimulated membrane ATPase. J Biol Chem 1992 ; 267: 4854-4858.
8. Noe J., Hagenbuch B., Meier PJ, St-Pierre MV,, et al. Characterization of the mouse bile salt export pump overexpressed in the baculovirus system. Hepatology 2001 ; 33: 1223-1231.
9. Bacso Z., Nagy H., Goda K., Bene L., Fenyvesi F., Matkó J.,, et al. Raft and cytoskeleton associations of an ABC transporter: P-glycoprotein. Cytometry A 2004 ; 61: 105-116.
10. Tietz P., Jefferson J., Pagano R., LaRusso NF: Membrane microdomains in hepatocytes: potential target areas for proteins involved in canalicular bile secretion. J Lipid Res 2005 ; 46: 1426-1432.
11. Pal A., Mehn D., Molnar ED, Gedey S., Meszaros P., Nagy T.,, et al. Cholesterol potentiates ABCG2 activity in a heterologous expression system: improved in vitro model to study function of human ABCG2. J Pharmacol Exp Ther 2007 ; 321: 1085-1094.
12. Telbisz A., Müller M., Ozvegy-Laczka C., Homolya L., Szente L., Váradi A.,, et al. Membrane cholesterol selectively modulates the activity of the human ABCG2 multidrug transporter. Biochim Biophys Acta 2007 ; 1768: 2698-2713.
13. Storch CH, Ehehalt R., Haefeli WE, Weiss J.,, et al. Localization of the human breast cancer resistance protein (BCRP/ABCG2) in lipid rafts/caveolae and modulation of its activity by cholesterol in vitro. J Pharmacol Exp Ther 2007 ; 323: 257-264.
14. Gimpl G., Klein U., Reilaender H., Fahrenholz F.: Expression of the human oxytocin receptor in baculovirus-infected insect cells: high-affinity binding is induced by a cholesterol-cyclodextrin complex. Biochemistry 1995 ; 34: 13794-13801.
15. Green RM, Hoda F., Ward KL: Molecular cloning and characterization of the murine bile salt export pump. Gene 2000 ; 241: 117-123.
16. Gerloff T., Stieger B., Hagenbuch B., Madon J., Landmann L., Rothi J.,, et al. The sister of P-glycoprotein represents the canalicular bile salt export pump of mammalian liver. J Biol Chem 1998 ; 273: 10046-10050.
17. Altmann F., Staudacher E., Wilson IBH, März L.: Insect cells as hosts for the expression of recombinant glycoproteins. Glycoconj J 1999 ; 16: 109-123.
18. Hayashi H., Takada T., Suzuki H., Onuki R., Hofmann AF, Sugiyama Y.: Transport by vesicles of glycine- and taurine-conjugated bile salts and taurolithocholate 3-sulfate: a comparison of human BSEP with rat Bsep. Biochim Biophys Acta 2005 ; 1738: 54-62.